RESUMEN
Investigating the role of biomolecules and bioactive molecules associated with membranes is fundamental to comprehend at the molecular point-of-view biochemical and clinical processes that occur at biointerfaces. In this paper we exploit the interaction of an intraocular dye solution based on lutein and zeaxanthin in surrogate internal limiting membrane (ILM) models, consisting of dipalmitoyphosphatidylcholine (DPPC) Langmuir monolayers, pure or mixed with collagen, proteoglycan and laminin. The interactions between the film components occurring at the air-water interface were investigated with surface pressure-area isotherms and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). A natural dye solution based on lutein and zeaxanthin, employed to label ILM in ophthalmic surgery, was incorporated in the ILM model, and the data suggested non-rupture of the structure of the membrane, with predominance of interactions based on intermolecular forces.
Asunto(s)
Colorantes/farmacología , Ojo/efectos de los fármacos , Luteína/farmacología , Membranas Artificiales , Modelos Teóricos , Xantófilas/farmacología , 1,2-Dipalmitoilfosfatidilcolina/química , Humanos , Presión , Soluciones , Espectrofotometría Infrarroja , Temperatura , ZeaxantinasRESUMEN
The authors report on two cases of tetralogy of Fallot (TF) associated with total anomalous pulmonary venous return to the coronary sinus. The clinical manifestations in both children were the same as of classical TF and diagnosis was confirmed by cineangiography. The patients underwent total surgical correction and the first one died in the immediate post-operative period. The second patient had an uneventful recovery. The rarity of this association is the main reason of this report where clinical and surgical findings are discussed.