Asunto(s)
Quirópteros/virología , Infecciones por Coronavirus/virología , Coronavirus/aislamiento & purificación , Reservorios de Enfermedades/virología , Animales , Brasil , Coronavirus/clasificación , Coronavirus/genética , Infecciones por Coronavirus/transmisión , Evolución Molecular , Humanos , FilogeniaRESUMEN
The present study aimed to investigate the frequency of pathogenic Leptospira spp. in Brazilian bats and to determine possible risk factors associated to it. Ninety two bats of 12 species were evaluated. Whole genomic DNA from kidneys was extracted and real-time PCR specific to pathogenic Leptospira spp. was applied. Association between the frequency of specimens positive for Leptospira spp. and sex, age, bat species or family, season of collection, geographic localization and feeding habits was evaluated. The results showed that 39.13% of analyzed bats were found positive for Leptospira spp. Nine bat species had at least one positive result. There was no association among the evaluated variables and frequency of pathogenic Leptospira spp. Although the limitations due to lack of Leptospira spp. isolation, leptospiral carriage was demonstrated in bats of different species from southern Brazil, which reinforces the need for surveillance of infectious agents in wild animals.
Asunto(s)
Quirópteros/microbiología , Leptospira/aislamiento & purificación , Leptospirosis/veterinaria , Animales , Brasil/epidemiología , Enfermedades Transmisibles Emergentes/epidemiología , Enfermedades Transmisibles Emergentes/microbiología , ADN Bacteriano/genética , Femenino , Genoma Bacteriano , Riñón/microbiología , Leptospira/genética , Leptospira/patogenicidad , Leptospirosis/epidemiología , Leptospirosis/microbiología , Masculino , Epidemiología Molecular , Salud Pública , Reacción en Cadena en Tiempo Real de la Polimerasa , Zoonosis/epidemiología , Zoonosis/microbiologíaRESUMEN
In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil.
Asunto(s)
Adenoviridae/aislamiento & purificación , Circovirus/aislamiento & purificación , Heces/virología , Lobos Marinos/virología , Animales , Brasil , ADN Viral/aislamiento & purificación , ARN Viral/aislamiento & purificaciónRESUMEN
This report describes the identification and characterization of a novel circovirus using metagenomic approaches in respiratory fluid samples from Brazilian free-tailed bats (Tadarida brasiliensis). The genome and deduced protein sequences share low identity with another circovirus recovered in distantly related bats from China.