RESUMEN

Brazil is renowned for its extensive plant biodiversity, with emphasis on Cymbopogon, C. citratus and C. nardus, with broad antimicrobial potential. Candidemias caused by Candida albicans are highly prevalent in immunosuppressed individuals and are associated with infections by biofilms on medical devices. The aim of this study was to evaluate the antimicrobial potential of essential oils C. citratus and C. nardus against C. albicans in planktonic and biofilm forms. Essential oils were obtained by hydrodistillation and chemical composition evaluated by GC-FID and GC-MS. The minimum inhibitory concentration was determined by the broth microdilution method and the synergy effect of essential oils and amphotericin B were evaluated by the checkerboard test. Biofilm activity was determined by the XTT assay. Cytotoxicity assays performed with VERO cells and molecular docking were performed to predict the effect of oil interaction on the SAP-5 enzyme site. The results showed activity of essential oils against planktonic cells and biofilm of C. albicans. Furthermore, the oils had a synergistic effect, and low cytotoxicity. Molecular docking showed interaction between Cadinene, Caryophyllen oxide, Germacrene D with SAP-5. The results indicate that Cymbopogon spp. studied are anti-Candida, with potential for further application in therapy against infections caused by C. albicans.

Asunto(s)

Antifúngicos , Biopelículas , Candida albicans , Cymbopogon , Pruebas de Sensibilidad Microbiana , Simulación del Acoplamiento Molecular , Aceites Volátiles , Cymbopogon/química , Aceites Volátiles/farmacología , Aceites Volátiles/química , Antifúngicos/farmacología , Antifúngicos/química , Candida albicans/efectos de los fármacos , Biopelículas/efectos de los fármacos , Animales , Células Vero , Chlorocebus aethiops , Cromatografía de Gases y Espectrometría de Masas

RESUMEN

OBJECTIVES: The aims of this study were to 1) examine individual professionals' perceptions of staffing risks and safe staffing in intensive care and 2) identify and examine the cognitive processes that underlie these perceptions. DESIGN: Qualitative case study methodology with nurses, doctors, and physiotherapists. SETTING: Three mixed medical and surgical adult ICUs, each on a separate hospital site within a 1,200-bed academic, tertiary London hospital group. SUBJECTS: Forty-four ICU team members of diverse professional backgrounds and seniority. INTERVENTIONS: None. MAIN RESULTS: Four themes (individual, team, unit, and organizational) were identified. Individual care provision was influenced by the pragmatist versus perfectionist stance of individuals and team dynamics by the concept of an "A" team and interdisciplinary tensions. Perceptions of safety hinged around the importance of achieving a "dynamic balance" influenced by the burden of prevailing circumstances and the clinical status of patients. Organizationally, professionals' risk perceptions affected their willingness to take personal responsibility for interactions beyond the unit. CONCLUSIONS: This study drew on cognitive research, specifically theories of cognitive dissonance, psychological safety, and situational awareness to explain how professionals' cognitive processes impacted on ICU behaviors. Our results may have implications for relationships, management, and leadership in ICU. First, patient care delivery may be affected by professionals' perfectionist or pragmatic approach. Perfectionists' team role may be compromised and they may experience cognitive dissonance and subsequent isolation/stress. Second, psychological safety in a team may be improved within the confines of a perceived "A" team but diminished by interdisciplinary tensions. Third, counter intuitively, higher "situational" awareness for some individuals increased their stress and anxiety. Finally, our results suggest that professionals have varying concepts of where their personal responsibility to minimize risk begins and ends, which we have termed "risk horizons" and that these horizons may affect their behavior both within and beyond the unit.

Asunto(s)

Actitud del Personal de Salud , Cultura , Unidades de Cuidados Intensivos , Seguridad del Paciente , Riesgo , Disonancia Cognitiva , Hospitales Universitarios , Humanos , Comunicación Interdisciplinaria , Colaboración Intersectorial , Londres , Grupo de Atención al Paciente , Teoría Psicológica , Investigación Cualitativa , Estudios Retrospectivos , Gestión de Riesgos , Recursos Humanos

Asunto(s)

Antígenos de Protozoos/sangre , Donantes de Sangre , ADN Protozoario/sangre , Leishmania infantum/genética , Leishmaniasis Visceral/epidemiología , Animales , Antígenos de Protozoos/inmunología , Enfermedades Asintomáticas , Brasil/epidemiología , ADN Protozoario/genética , Ensayo de Inmunoadsorción Enzimática , Humanos , Insectos Vectores/parasitología , Leishmania infantum/aislamiento & purificación , Leishmaniasis Visceral/sangre , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/transmisión , Reacción en Cadena de la Polimerasa , Prevalencia , Psychodidae/parasitología

RESUMEN

OBJECTIVES: Research suggests that better feedback from quality and safety indicators leads to enhanced capability of clinicians and departments to improve care and change behaviour. The aim of the current study was to investigate the characteristics of feedback perceived by clinicians to be of most value. METHODS: Data were collected using a survey designed as part of a wider evaluation of a data feedback initiative in anaesthesia. Eighty-nine consultant anaesthetists from two English NHS acute Trusts completed the survey. Multiple linear regression with hierarchical variable entry was used to investigate which characteristics of feedback predict its perceived usefulness for monitoring variation and improving care. RESULTS: The final model demonstrated that the relevance of the quality indicators to the specific service area (ß=0.64, p=0.01) and the credibility of the data as coming from a trustworthy, unbiased source (ß=0.55, p=0.01) were the significant predictors, having controlled for all other covariates. CONCLUSION: For clinicians to engage with effective quality monitoring and feedback, the perceived local relevance of indicators and trust in the credibility of the resulting data are paramount.

Asunto(s)

Anestesiología/organización & administración , Retroalimentación , Indicadores de Calidad de la Atención de Salud/organización & administración , Anestesiología/normas , Competencia Clínica , Estudios Transversales , Inglaterra , Investigación sobre Servicios de Salud , Humanos , Cultura Organizacional , Indicadores de Calidad de la Atención de Salud/normas , Medicina Estatal/organización & administración

RESUMEN

The effects promoted by environmental aqueous matrices on pesticide determinations have been assessed, and for the first time, a simple, low-cost and efficient strategy for the correction of analytical results has been determined. This method can be useful as a parameter of quality control in a quality assurance programs. Evaluation of the matrix effect showed that environmental aqueous matrices, e.g., estuarine water, promote a distinctive and significant effect on the determination of pesticides. The picloram, atrazine and methyl parathion pesticides suffered the smallest effects promoted by the estuarine matrix, whereas chlorpyrifos and cypermethrin suffer a significant effect. For picloram, the matrix effect was a function of its physiochemical properties. However, for atrazine, methyl parathion, chlorpyrifos and cypermethrin, the matrix effect was promoted by environmental matrix components. As strategy for analytical quality control, it has been determined that there are relation factors (RFs) between pesticides and the selected surrogates standards. These RFs are not altered by the complexities and compositions of simple and complex aqueous matrices. Predetermined RFs was applied to the picloram, atrazine and methyl parathion assessment in a real sample from the estuary of the Jaguaribe River, and the results showed that when no quality control was applied, the concentration levels would be underestimated, leading to incorrect results and inaccurate conclusions.

Asunto(s)

Técnicas de Química Analítica/normas , Ambiente , Contaminantes Ambientales/análisis , Plaguicidas/análisis , Agua/química , Control de Calidad

RESUMEN

The aim of this study was to investigate HIV-1 molecular diversity and the epidemiological profile of HIV-1-infected patients from Ribeirão Preto, Brazil. A nested PCR followed by sequencing of a 302-base pair fragment of the env gene (C2-V3 region) was performed in samples from HIV-1-positive patients. A total of 45 sequences were aligned with final manual adjustments. The phylogenetic analyses showed a higher prevalence of HIV-1 subtype B in the studied population (97.8%) with only one sample yielding an F1 subtype. The viral genotyping prediction showed that CCR5 tropism was the most prevalent in the studied cohort. Geno2pheno analysis showed that R5 and CXCR4 prediction were 69% and 31%, respectively. There was no statistical significance, either in viral load or in CD4(+) T cell count when R5 and X4 prediction groups were compared. Moreover, the GPGR tetramer was the most common V3 loop core motif identified in the HIV-1 strains studied (34.1%) followed by GWGR, identified in 18.1% of the samples. The high level of B subtype in this Brazilian population reinforces the nature of the HIV epidemic in Brazil, and corroborates previous data obtained in the Brazilian HIV-infected population.

Asunto(s)

Linfocitos T CD4-Positivos/virología , Regiones Determinantes de Complementariedad/genética , Genes env , Proteína gp120 de Envoltorio del VIH/genética , Infecciones por VIH/epidemiología , VIH-1/genética , Adolescente , Adulto , Secuencia de Bases , Brasil/epidemiología , Linfocitos T CD4-Positivos/inmunología , Estudios de Cohortes , Regiones Determinantes de Complementariedad/inmunología , Epidemias , Femenino , Genotipo , Proteína gp120 de Envoltorio del VIH/inmunología , Infecciones por VIH/inmunología , Infecciones por VIH/virología , VIH-1/clasificación , VIH-1/inmunología , VIH-1/aislamiento & purificación , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Receptores CCR5/genética , Receptores CCR5/inmunología , Receptores CXCR/genética , Receptores CXCR/inmunología , Carga Viral

RESUMEN

STUDY OBJECTIVES: Evaluation of the combined use of polymerase chain reaction (PCR) and adenosine deaminase (ADA) activity on the diagnosis of pleural tuberculosis (pTB) in a region of high prevalence of tuberculosis. PATIENTS: PCR and determination of ADA activity were performed on the pleural fluid of every patient presenting with pleural effusion suspected to be associated with tuberculosis. The case definition of pTB involved parameters including the combination of clinical and radiologic findings; biochemical, microbiologic, and cytologic examination of the pleural fluid; and the histopathologic findings of pleural fragments obtained by biopsy. The diagnosis of pTB was confirmed in any patient presenting with positive culture findings of Mycobacterium tuberculosis, either on the pleural fluid or other biological material, or the presence of histopathologic findings suggestive of pTB on pleural biopsy, and also, in the absence of negative laboratory results, those patients with clinical improvement after empirical treatment. RESULTS: We studied 45 patients with pleural effusion. Of these, 16 patients met the diagnosis of pTB by our broad case definition. PCR findings were positive in six patients. The reaction was also positive in a patient whose diagnosis of tuberculosis could not be confirmed. ADA activity was considered positive in 11 patients with pTB. The combined use of PCR and ADA activity confirmed pTB in 14 patients; however, when analyzed in combination with the conventional methods, diagnosis of pTB was achieved in all 16 patients. CONCLUSION: Our results show that, even in a highly endemic area, neither PCR nor ADA activity should be relied on as a single test that substitutes for the diagnostic methods already available, but rather they should be used as an extra tool in the diagnosis of pTB. The combined analysis of PCR and ADA activity, however, is a very useful diagnostic approach to achieve a more rapid and precise diagnosis in the cases of pTB.

Asunto(s)

Adenosina Desaminasa/metabolismo , Enfermedades Endémicas , Derrame Pleural/enzimología , Reacción en Cadena de la Polimerasa , Tuberculosis Pleural/diagnóstico , Antituberculosos/uso terapéutico , Brasil , Femenino , Humanos , Masculino , Pleura/patología , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Esputo/microbiología , Tuberculosis Pleural/tratamiento farmacológico , Tuberculosis Pleural/patología

RESUMEN

Objetivo: Relatar a ocorrência de pancitopenia transitória, decorrente de infecção pelo parvovírus B19, em um paciente portador de anemia hemolíitica hereditária e comentar a importância do diagnóstico desta infecção. Métodos: Relato de caso clínico acompanhado pelos autores, diagnosticado sorologicamente e pelo método da reação em cadeia da polimerase (PCR), e revisão da literatura. Resultados: Menino de 12 anos, portador de esferocitose hereditária, apresentando quadro infeccioso inespecífico seguido de pancitopenia grave, transitória, com diagnóstico de infecção por parvovírus B19. Conclusões: O diagnóstico da infecção por parvovírus B19 é de particular importância em hematologia, principalmente quando estão presentes algumas condições mórbitas, entre elas as anemias hemolíticas hereditárias, sendo o método de PCR útil por permitir rapidez e boa sensibilidade no diagnóstico específico desta patologia

Asunto(s)

Humanos , Masculino , Niño , Anemia Aplásica , Anemia Hemolítica , Pancitopenia , Parvovirus B19 Humano , Esferocitosis Hereditaria