RESUMEN
Objective: To study the chemical constituents of the stems from Altingia chinensis. Methods: The stems from Altingia chinensis were extracted with 95% ethanol for reflux,and the extract were evaporated. The chemical constituents were isolated by silica gel chromatography and Sephadex LH-20 column chromatography from petrol ether part and ethyl acetate part of extract. Their structures were identified on the basis of physico-chemical characters and spectroscopic analysis. Results: Eleven compounds were obtained from the stems from Altingia chinensis,which identified as myrsinene( 1),oleanonic aldehyde( 2),3ß,23,28-trihydroxyolean-12-ene( 3),ursolic acid-3ß-octadecanoate( 4),arjunglucoside â ¡( 5),ß-sitosterol( 6),daucosterol( 7),trans-resveratrol-3-O-ß-D-glucopyranoside( 8),ellagic acid 3,3'-dimethylether( 9),lyoniside( 10) and 3,3'-O-dimethylellagic acid-4'-O-α-L-rhamnoside( 11). Conclusion: All the compounds are isolated from Altingia chinensis for the first time.
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Saxifragaceae , Ácido Elágico , Sitoesteroles , Triterpenos , Ácido UrsólicoRESUMEN
The aim of the present study was to determine the most meaningful preoperative prognostic factor of cancer-related death in ovarian cancer patients by comparing potentially prognostic systemic inflammatory response (SIR) markers. The levels of fibrinogen, albumin, C-reactive protein (CRP), and serum cancer antigen-125 (CA-125) and the neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) were evaluated in 190 ovarian cancer patients to identify predictors of overall survival (OS) and progression-free survival (PFS) using univariate and multivariate analyses. Patients with a PLR >203 had a shorter PFS and OS than the patients in PLR ≤203 group (11 vs. 24 months and 28 vs. 64 months). Univariate analyses revealed that tumor stage, postoperative residual tumor mass, ascites, and the levels of all SIR markers were associated with PFS and OS. Multivariate analysis revealed that PLR was independently associated with PFS (hazard ratio [HR] 1.852, 95% confidence interval [CI] 1.271-2.697, P = 0.001) and OS (HR 2.158, 95%CI 1.468-3.171, P < 0.001), as well as tumor stage and postoperative residual tumor mass. In contrast, fibrinogen remained significant only for PFS (HR 1.724, 95%CI 1.197-2.482, P = 0.003). Patients with a PLR >203 were more prone to have advanced tumor stage (P = 0.002), postoperative residual tumor mass >2 cm (P = 0.032), malignant ascites (P < 0.001), and all the other elevated SIR markers (P < 0.001). Preoperative PLR is superior to other SIR markers (CA-125, NLR, fibrinogen, CRP, and albumin) as a predictor of survival in ovarian cancer patients.
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Biomarcadores/sangre , Plaquetas , Inflamación/sangre , Linfocitos , Neoplasias Ováricas/sangre , Adulto , Anciano , Proteína C-Reactiva/metabolismo , Supervivencia sin Enfermedad , Femenino , Humanos , Inflamación/patología , Estimación de Kaplan-Meier , Persona de Mediana Edad , Neoplasias Ováricas/patología , PronósticoRESUMEN
OBJECTIVE: To study the chemical constituents from the roots of Semiliquidambar cathayensis. METHODS: The roots of Semiliquidambar cathayensis were extracted with 80% ethanol for reflux. Chemical constituents were isolated by silica gel chromatography and Sephadex LH-20 column chromatography from petrol ether part and ethyl acetate part of extracts. Their structures were identified on the basis of physico-chemical characters and spectroscopic analysis. RESULTS: Eleven compounds were obtained from the roots of Semiliquidambar cathayensis, and identified as 3-acetyl-12-ene-oleanolic acid methyl ester (1), ß-sitosterol (2), 3-acetyl-12-ene-oleanol-ic acid (3), 2α,3ß-dihydroxy-lup-20(29)-en-28-oic acid (4), (24R)-5α-stignast-3,6-dione (5), betulonic acid (6), stearic acid (7), hexadecanoic acid (8), 3-oxo-olean-12-en-28-oic acid (9), arjunolic acid (10) and daucosterol (11). CONCLUSION: Compounds 1,3 - 6 and 8 are isolated from this genus for the first time.
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Hamamelidaceae/química , Fitoquímicos/análisis , Raíces de Plantas/química , Plantas Medicinales/química , Extractos Vegetales/químicaRESUMEN
OBJECTIVE: To identify the differentially expressed proteins in the serum of patients with cervical cancer for use as the biomarkers for early diagnosis of cervical cancer. METHODS: Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) with weak cationic chips (CM10) was used to examine the serum samples of 24 patients with cervical squamous cell carcinoma and 25 age-matched healthy women. The protein fingerprints were obtained, and bioinformatic analysis was performed to identify the differentially expressed proteins in the serum of the patients. RESULTS: Fifty-two differentially expressed proteins were detected in the serum of cervical cancer patients (P<10(-5)), among which 6 proteins with mass/charge ratio of 4173.77, 5903.09, 6087.12, 10716.9, 6109.61 and 3397.41, respectively, showed lowered expression in the serum of cervical cancer patients. Two diagnostic models for cervical cancer were generated using software, including one consisting of the 4173.77(M/Z) protein with the diagnostic specificity of 96% and sensitivity of 75% for cervical cancer and the other consisting of 3 proteins at 5335.81(M/Z), 7562.99(M/Z), and 9287.89(M/Z) with specificity of 91.67% and sensitivity of 96.0%. CONCLUSION: Cervical cancer patients show different serum protein expression profile from healthy women. The 6 differential proteins identified may serve as specific serum biomarkers in close relation to the origin and progression of cervical cancer.
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Biomarcadores de Tumor/sangre , Proteínas Sanguíneas/análisis , Carcinoma de Células Escamosas/sangre , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Neoplasias del Cuello Uterino/sangre , Adulto , Anciano , Carcinoma de Células Escamosas/diagnóstico , Femenino , Humanos , Persona de Mediana Edad , Sensibilidad y Especificidad , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Neoplasias del Cuello Uterino/diagnósticoRESUMEN
BACKGROUND & OBJECTIVE: Upward (local growth and invasion of the base of skull), downward (distant metastasis) and mixed progressing types of nasopharygeal carcinoma (NPC) have been observed when the disease progress to middle-late stage. The upward and downward progressing types are evidently different in clinical symptom, therapy strategy and prognosis. Identification of the molecular differences between them is very important for molecular classification, prognostic prediction and research on neoplasia and development of NPC. This study was to discover the genes differentially expressed in upward and downward progressing types of NPC. METHODS: An oligo gene chip containing 21 300 genes was used to detect the genes differentially expressed between upward and downward progressing types of NPC. One of the differentially expressed genes detected was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Seventeen genes were differentially expressed between upward and downward progressing types of NPC. The expression of SELB, Clorf29, GLE1L and FLJ20989 genes were up-regulated and the expression of 1D12A, ASPN, DCN, PRO2219, LRDD, DIO2, ULBP2, PRO3073, IGVH3, IGVH4, IGLJ3, PRO0943 and AK057247 genes were down-regulated in the upward progressing type as compared with those in the downward progressing type. The difference of gene expression was ranged from 2.30 to 4.23 folds. The high expression rate of DIO2 gene was significantly higher in downward progressing type than in upward progressing type (90.0% vs. 33.3%, P = 0.020). CONCLUSIONS: The gene expression patterns are significantly different between upward and downward progressing types of NPC. The expression of DIO2 gene is higher in the downward progressing type than in upward progressing type, which may be closely related to the metastasis potential of NPC.
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Perfilación de la Expresión Génica , Yoduro Peroxidasa/metabolismo , Neoplasias Nasofaríngeas/genética , Neoplasias Nasofaríngeas/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Adulto , Anciano , Carcinoma de Células Escamosas/clasificación , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Masculino , Persona de Mediana Edad , Neoplasias Nasofaríngeas/clasificación , Neoplasias Nasofaríngeas/patología , Metástasis de la Neoplasia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Neoplasias de la Base del Cráneo/genética , Neoplasias de la Base del Cráneo/metabolismo , Neoplasias de la Base del Cráneo/secundario , Yodotironina Deyodinasa Tipo IIRESUMEN
OBJECTIVE: To evaluate the effects of topical DMSO and intralesional hyaluronidase administration, used alone or in combination, on skin injury due to vinorelbine extravasation in rats. METHODS: Skin injury due to vinorelbine extravasation was induced in the lower extremities of 30 SD rats, which were treated subsequently with topical DMSO, intralesional hyaluronidase, their combination, topical saline, and intralesional saline, with the rats without any treatment as the control. The wound area on 1, 4, 8, 12, 18, 24, 30 days and the time of healing were observed and compared. RESULTS: The wound area on 1, 4, 8, 12, 18, and 24 days were significantly smaller in topical DMSO group than in topical saline and control groups (P<0.05), and so in intralesional hyaluronidase group than in intralesional saline and control groups (P<0.05), but there was no significant difference between single agent (hyaluronidase and DMSO) treatment group and the combined treatment group. The healing time was significantly shorter in topical DMSO and intralesional hyaluronidase groups than in topical and intralesional saline groups and control group ( 24.9-/+3.2 and 21.9-/+3.0 days vs 29.8-/+2.6, 28.6-/+4.1 and 30.6-/+3.0 days, P<0.01), but comparable between the two single agent groups and combined treatment group (23.3-/+3.8 days). CONCLUSION: Intralesional hyaluronidase and topical DMSO application are effective therapies for skin damage due to vinorelbine extravasation, and their combination does not improve the therapeutic effect.
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Dimetilsulfóxido/administración & dosificación , Dimetilsulfóxido/farmacología , Hialuronoglucosaminidasa/administración & dosificación , Hialuronoglucosaminidasa/farmacología , Piel/efectos de los fármacos , Piel/lesiones , Vinblastina/análogos & derivados , Administración Tópica , Animales , Quimioterapia Combinada , Femenino , Inyecciones Intralesiones , Masculino , Ratas , Piel/patología , Factores de Tiempo , Vinblastina/farmacología , Vinorelbina , Cicatrización de Heridas/efectos de los fármacosRESUMEN
OBJECTIVE: To explore the inhibitory effect of garlic oil on cyclin E expression in gastric adenocarcinoma cells. METHODS: Human gastric adenocarcinoma SGC7901 cells were cultured routinely and the expressions of transforming growth factor alpha (TGFalpha) and epidermal growth factor receptor (EGFR) are detected by immunofluorescent staining and flow cytometry. The SGC7901 cells were also cultured with RPMI 1640 without calf serum for 48 h, followed by further culture with RPMI 1640 in the presence of 2.5% calf serum before treatment with TGFalpha, garlic oil, or their combination, and cyclin E expression of the cells was then detected by immunofluorescent staining and flow cytometry. RESULTS: The positivity rates of TGFalpha and EGFR expressions were 46.80% and 57.78 % respectively in SGC7901 cells cultured routinely for 48 h. The positivity rate of cyclin E expression was increased by 7.06% (P<0.001) in SGC7901 cells treated with 30 microg/L TGFalpha for 5 h, decreased by 11.75% (P<0.001) following a 5-hour treatment with 10% garlic oil, and decreased further by 17.11% (Plt;0.001) after treatment with both 30 microg/L TGFalpha and 10% garlic oil for 5 h. CONCLUSIONS: The gastric adenocarcinoma SGC7901 cells express TGFalpha and EGFR and possess TGFalpha autocrine and paracrine loops to promote cell proliferation. Garlic oil inhibits cyclin E expression in routinely cultured SGC7901 cells and also in TGFalpha-treated ones, suggesting that garlic oil can inhibit the TGFalpha autocrine and paracrine loops, which can be one of the pathways of garlic oil to inhibit cancer cell proliferation.
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Adenocarcinoma/patología , Compuestos Alílicos/farmacología , Antineoplásicos/farmacología , Ciclina E/metabolismo , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Neoplasias Gástricas/patología , Sulfuros/farmacología , Adenocarcinoma/genética , Animales , Línea Celular Tumoral , Regulación hacia Abajo/efectos de los fármacos , Receptores ErbB/metabolismo , Humanos , Neoplasias Gástricas/genética , Factor de Crecimiento Transformador alfa/metabolismoRESUMEN
BACKGROUND & OBJECTIVE: Docetaxel extravasating into the surrounding tissues may lead to severe skin damage. No guideline for handling docetaxel extravasation has been proposed till now. This study was to explore the efficacy of chitosan and hyaluronidase on skin damage caused by docetaxel extravasation in a rat model. METHODS: A docetaxel extravasation model was established in both lower extremities of 30 Sprague-Dawley rats. The rats were divided into 6 groups and received chitosan embrocation, hyaluronidase injection, hyaluronidase injection plus chitosan embrocation, saline embrocation, or saline injection, or received no treatment as control. The occurrence rate and extent of skin damage, and the healing time were observed and compared. RESULTS: The occurrence rate of skin damage was significantly lower in hyaluronidase group and hyaluronidase plus chitosan group than in chitosan group, saline embrocation group, saline injection group, and control group (30% and 20% vs. 90%, 100%, 90% and 100%, P<0.05). The healing time was significantly shorter in hyaluronidase group and hyaluronidase plus chitosan group than in chitosan group, saline embrocation group, saline injection group, and control group [(12.00+/-3.00) days and (9.50+/-2.12) days vs. (18.33+/-2.00) days, (23.70+/-2.41) days, (18.44+/-2.01) days and (25.70+/-2.26) days, P<0.01], and was significantly shorter in chitosan group than in saline embrocation group and control group (P<0.01). CONCLUSIONS: Hyaluronidase alone or hyaluronidase plus chitosan could decrease the occurrence rate of skin damage caused by docetaxel extravasation in rats, and shorten the healing time. Chitosan embrocation can improve the damage healing, but cannot decrease the occurrence rate of skin damage.
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Quitosano/uso terapéutico , Hialuronoglucosaminidasa/uso terapéutico , Úlcera Cutánea/tratamiento farmacológico , Taxoides/efectos adversos , Administración Cutánea , Animales , Antineoplásicos/efectos adversos , Docetaxel , Quimioterapia Combinada , Extravasación de Materiales Terapéuticos y Diagnósticos/tratamiento farmacológico , Extravasación de Materiales Terapéuticos y Diagnósticos/etiología , Femenino , Inyecciones , Masculino , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Úlcera Cutánea/inducido químicamenteRESUMEN
The endothelin A receptor (ET(A)R) autocrine pathway is overexpressed in many malignancies, including nasopharyngeal carcinoma (NPC). In this tumor, ET(A)R expression is an independent determinant of survival and a robust independent predictor of distant metastasis. To evaluate whether ET(A)R represents a new target in NPC treatment, we tested the therapeutic role of ET(A)R in NPC. Cell proliferation was inhibited by the ET(A)R-selective antagonist ABT-627 in two ET(A)R-positive NPC cells in a dose-dependent manner. Proliferation of ET(A)R-negative NPC cells was not decreased. ET(A)R blockade also resulted in sensitization to cisplatin and 5-fluorouracil-induced apoptosis. In nude mice, ABT-627 inhibited the growth of NPC cell xenografts. Combined treatment of ABT-627 with the cytotoxic drug cisplatin or 5-fluorouracil produced additive antitumor effects. The antitumor activity of ABT-627 was demonstrated finally on an experimental lung metastasis by a reduction in the number of tumors. These results support the rationale of combining ABT-627 with current standard chemotherapy to further improve the therapeutic ratio in the treatment of NPC.
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Antagonistas de los Receptores de la Endotelina A , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Nasofaríngeas/tratamiento farmacológico , Animales , Antimetabolitos Antineoplásicos/uso terapéutico , Antineoplásicos/uso terapéutico , Apoptosis/efectos de los fármacos , Atrasentán , Proliferación Celular/efectos de los fármacos , Cisplatino/uso terapéutico , Modelos Animales de Enfermedad , Quimioterapia Combinada , Antagonistas de los Receptores de la Endotelina B , Endotelina-1/metabolismo , Fluorouracilo/uso terapéutico , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/secundario , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patología , Pirrolidinas/uso terapéutico , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Neoplásico/genética , ARN Neoplásico/metabolismo , Receptor de Endotelina A/genética , Receptor de Endotelina A/metabolismo , Receptor de Endotelina B/genética , Receptor de Endotelina B/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Tumorales Cultivadas , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
OBJECTIVE: To explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells. METHODS: Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 microg/L TGFalpha for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM. RESULTS: The percentage of the cells at G(0)/G(1) phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFalpha treatment of the cells synchronized at G(0)/G(1) phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFalpha of 50 microg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001). CONCLUSION: TGFalpha can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.
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Ciclina D1/biosíntesis , Ciclina E/biosíntesis , Factor de Crecimiento Transformador alfa/farmacología , Adenocarcinoma/metabolismo , Adenocarcinoma/patología , Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Citometría de Flujo , Técnica del Anticuerpo Fluorescente , Humanos , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologíaRESUMEN
BACKGROUND & OBJECTIVE: Pre-symptomatic screening of early-stage breast cancer may greatly reduce tumor-related mortality. Some tumor markers, such as CA15-3 and CA27-29, are recommended only for monitoring therapy of advanced or relapsed breast cancer. This study was to find new biomarkers that could be used individually or in combination with an existing modality for cost-effective screening of breast cancer by proteome analysis. METHODS: Protein expression differences among 128 serum samples of 64 breast cancer patients (19 of stage I, 24 of stage II, and 21 of stage III), 52 patients with benign breast diseases, and 12 healthy women were analyzed with IMAC3 and WCX2 Ciphergen ProteinChip Arrays. RESULTS: On WCX2 chip, a panel of 5 proteins (9 116, 8 905, 8 749, 9 470, and 9 692 Da) was selected based on their collective contribution to the optimal separation between breast cancer patients and both non-cancer patients and healthy women, and expression of another 2 proteins (9 405 and 6 424 Da) was different between patients with breast cancer of stage I and stage III. On IMAC3 chip, a panel of 9 proteins (5 236, 7 823, 7 464, 5 213, 5 334, 5 064, 5 374, 7 756, and 7 623 Da) was selected based on their collective contribution to the optimal separation between breast cancer patients and both non-cancer patients and healthy women, and expression of another 3 proteins (7 922, 4 641, and 5 910 Da) was different between patients with breast cancer of stage I and stage III. CONCLUSION: Protein expression in breast cancer patients is different from that in both non-cancer patients and healthy women, and those proteins with different expression may be used as new biomarkers in breast cancer.
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Biomarcadores de Tumor/sangre , Neoplasias de la Mama/sangre , Proteoma/análisis , Adulto , Neoplasias de la Mama/patología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Análisis por Matrices de ProteínasRESUMEN
AIM: To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV). METHODS: Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied. RESULTS: Two biomarkers with M(r) 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients, a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained. CONCLUSION: These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression.
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Virus de la Hepatitis B/aislamiento & purificación , Hepatitis B/diagnóstico , Cirrosis Hepática/diagnóstico , Análisis por Matrices de Proteínas/métodos , Biomarcadores/sangre , Carcinoma Hepatocelular/sangre , Carcinoma Hepatocelular/diagnóstico , Portador Sano , Estudios de Cohortes , Diagnóstico Diferencial , Hepatitis B/sangre , Humanos , Cirrosis Hepática/sangre , Neoplasias Hepáticas/sangre , Neoplasias Hepáticas/diagnóstico , Masculino , Valor Predictivo de las Pruebas , Valores de Referencia , Sensibilidad y Especificidad , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodosRESUMEN
BACKGROUND & OBJECTIVE: Previous studies have indicated that increased expression of TGFalpha and cyclin E are correlated with the oncogenesis and progress of cancer; however, their expression patterns in gastric precancerous lesions have been not clear yet. In addition, the association between expression of TGFalpha and cyclin E has not been reported. This study was designed to investigate the expression of TGFalpha and cyclin E in chronic superficial gastritis (CSG), gastric precancerous lesions, and gastric carcinoma (GCA), and analyze the association of TGFalpha and cyclin E expression, and the relationship between their expression and different development stages of oncogenesis of GCA. METHODS: The expression of TGFalpha and cyclin E in CSG, intestinal metaphases (IM), atypical hyperplasia (AH), and GCA were examined using immunohistochemical staining. The association of TGFalpha and cyclin E expression was also statistically analyzed. RESULTS: The expression rates of TGFalpha and cyclin E were 15.1%, 53.6%, 51.7%, 61.7%, and 7.5%, 28.6%, 37.9%, 42.6% in tissue samples of CSG, IM, AH and GCA, respectively. The positive expression rates of TGFalpha and cyclin E were higher in IM, AH, GCA than in CSG; the difference was significant (each P< 0.05). In addition, the positive expression rates of TGFalpha and cyclin E were higher in low differential adenocarcinoma (TGFalpha 81.0%, cyclin E 57.1%) than in mediate to high differential one (TGFalpha 41.7%, cyclin E 16.7%) (P< 0.05,for both TGFalpha and cyclin E). It is also revealed that the expression of TGFalpha and cyclin E were closely associated (P< 0.001 for the tissue of gastric chronic inflammation, and P=0.005 for gastric carcinoma, respectively). CONCLUSION: In the tissues of CSG, IM, AH, and GCA, the expression rates of TGFalpha and cyclin E are increased gradually with the severity of the pathological lesions and the malignancy of GCA. In addition, the expression of TGFalpha and cyclin E were obviously associated.
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Ciclina E/análisis , Lesiones Precancerosas/química , Neoplasias Gástricas/química , Factor de Crecimiento Transformador alfa/análisis , Adolescente , Adulto , Anciano , Enfermedad Crónica , Femenino , Gastritis/metabolismo , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana EdadRESUMEN
BACKGROUND & OBJECTIVE: We cloned NP9 gene (GenBank, BF718797) in a previous study which was down-regulated in nasopharyngeal carcinoma (NPC). To clarify the function of NP9 gene, we cloned the coding sequence (CDS) of NP9 gene and investigated the influence of NP9 on cyclin D1 expression. METHODS: A full-length cDNA sequence was obtained by Blast NP9 EST, then the complete CDS was cloned into an eukaryotic expressing vector pRc/CMV2. The plasmid was transfected into CNE1 cells (NPC cells) with lipofectamine and positive cell clones which stably expressed NP9 CDS were established by G418 screening. The luciferase report plasmid with cyclin D1 promoter or NF-kappaB-Luc report plasmid was transfected into positive clones and the luciferase activity was detected. At last, the NP9 CDS was subcloned into pEGFP-C1 and cellular localization of NP9 protein was observed through transfecting pEGFP-C1-NP9 into CNE1 cells. RESULTS: The fusion GFP was located in cell nuclei. The positive cell clones screened by G418 expressed NP9 CDS at different levels. Compared with control, the luciferase activities in NP9 positive clones were decreased 46% and 63% after 48 hours of transfecting the luciferase report plasmid with cyclin D1 promoter or NF-kappaB-Luc plasmid. CONCLUSION: NP9 protein is a nuclear protein. NP9 gene can down-regulate the transcription activity of cyclin D1 and NF-kappaB.