RESUMEN

Plasmid pSP124S with ble gene was transformed into D. salina cell by electroporation. The retention and expression of foreign gene in D.salina was explored, proper parameters were established. A large amount of foreign plasmid was found to be introduced into cells. The plasmid was degraded gradually, but can be detected within 96 hours. The ble gene was efficiently transcribed from foreign promoter and transcription was maintained at least 72 hours. The ble gene can be translated correctly and so can be used as a selectable marker for the research of genetic tranformation in D. salina.

Asunto(s)

Proteínas Bacterianas/metabolismo , Chlorophyta/metabolismo , Proteínas Bacterianas/genética , Western Blotting , Chlorophyta/genética , Electroporación , Plásmidos/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa