RESUMEN
N6-methyladenosine (m6A) alteration is an epigenetic regulator widely involved in the tumorigenicity of hepatocellular carcinoma (HCC). The role of YTH N6-methyladenosine RNA binding protein F3 (YTHDF3), an m6A reader in HCC, requires further investigation. Here, we aim to explore the biological properties of YTHDF3 in HCC and its potential mechanisms. The predictive risk model for HCC was developed by analyzing the expression of genes associated with m6A in HCC using online datasets. WB and qPCR were employed to assess YTHDF3 expression in HCC and its correlation with the disease's clinicopathological characteristics. Both in vitro and in vivo methods were utilized to evaluate the biological effects of YTHDF3 in HCC. The potential targets of YTHDF3 were identified and confirmed using RNA-seq, meRIP-seq, and linear amplification and sequencing of cDNA ends (Lace-seq). We confirmed that YTHDF3 is overexpressed in HCC. Patients with higher YTHDF3 expression had a greater risk of cancer recurrence. In both in vitro and in vivo settings, YTHDF3 boosts the migration and invasion capabilities of HCC cells. Through multi-omics research, we identified YTHDF3's downstream target genes as NKD inhibitors of the WNT signaling pathway 1 (NKD1) and the WNT/ß-catenin signaling pathway. With m6A modification, YTHDF3 suppresses the transcription and translation of NKD1. Additionally, NKD1 inhibited tumor growth by blocking the WNT/ß-catenin signaling pathway. The investigation found that the oncogene YTHDF3 stimulates the WNT/ß-catenin signaling pathway by m6A-dependently suppressing NKD1 expression in HCC cells. Our findings suggest that YTHDF3 regulates hepatocarcinogenesis, providing fresh perspectives on potential biomarkers and therapeutic targets for HCC.
Asunto(s)
Adenosina , Carcinoma Hepatocelular , Regulación Neoplásica de la Expresión Génica , Neoplasias Hepáticas , Invasividad Neoplásica , Proteínas de Unión al ARN , Vía de Señalización Wnt , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patología , Carcinoma Hepatocelular/metabolismo , Neoplasias Hepáticas/patología , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Humanos , Proteínas de Unión al ARN/metabolismo , Proteínas de Unión al ARN/genética , Vía de Señalización Wnt/genética , Animales , Ratones , Adenosina/análogos & derivados , Adenosina/metabolismo , Regulación Neoplásica de la Expresión Génica/genética , Invasividad Neoplásica/genética , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Proteínas Adaptadoras Transductoras de Señales/genética , Proliferación Celular/genética , Movimiento Celular/genética , Línea Celular Tumoral , Ratones Desnudos , beta Catenina/metabolismo , beta Catenina/genética , Ratones Endogámicos BALB C , Masculino , Metástasis de la Neoplasia , Proteínas de Unión al CalcioRESUMEN
OBJECTIVE: To investigate the varieties, resoures and identification authentication of Guoshangye used in Tuja and Miao's folk. METHOD: Through field investigations and comparing the collected specimens and literatures, the classification and identification of the species were studied. RESULT AND CONCLUSION: Origin of Guoshangye plant was from Pholidota yunnanensis, Bulbophyllum andersonii, B. odoratissimum, B. kamgtimgemse, ect. their morphological characters were identified. Due to limited resource of Guoshangye, the development and protection should be paid more attention.
Asunto(s)
Orchidaceae/anatomía & histología , Plantas Medicinales/anatomía & histología , Analgésicos/farmacología , Animales , Antitusígenos/farmacología , Conservación de los Recursos Naturales , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Historia del Siglo XIX , Historia Medieval , Humanos , Orchidaceae/química , Orchidaceae/clasificación , Farmacognosia/historia , Raíces de Plantas/anatomía & histología , Raíces de Plantas/química , Plantas Medicinales/química , Plantas Medicinales/clasificaciónRESUMEN
OBJECTIVE: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR. METHOD: the electrophoresis of total DNA, UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods. RESULT: The DNA concentration and yields were different, which greatly influenced the result of RAPD-PCR. CONCLUSION: The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.