RESUMEN
Introduction: Intercropping practices play a crucial role in enhancing and maintaining the biodiversity and resiliency of agroecosystems, as well as promoting stable and high crop yields. Yet the relationships between soil nitrogen, microbes, and yield in maize cultivated under maize/soybean intercropping systems remain unclear. Methods: To fill that knowledge gap, here we collected maize rhizosphere soil at the staminate stage after 6 consecutive years of maize/soybean intercropping, to investigate how intercropping and nitrogen application rates affected nitrogen utilization by crops and soil microbial community composition and function. We also examined correlations of those responses with yields, to clarify the main ways that yield is enhanced via intercropping and by nitrogenous fertilizer gradient changes generated by different nitrogen application rates. Results: The amount of applied fertilizer was 240 kg N ha-1 was best for obtaining a high maize yield and also led to the greatest nitrogen-use efficiency and bacterial diversity. Under the same N application rate, intercropping increased the maize yield by 31.17% and soil nitrogen (total, ammonium and nitrate nitrogen) by 14.53%, on average, in comparison to monocropping. The enrichment of Gemmatimonas and Bradyrhizobium significantly increased the soil nitrogen content, and a greater relative abundance of Sphingomonas and Gemmatimonas increased the maize yield, whereas enrichment of Candidatus_Udaeobacter and Bradyrhizobium decreased it. The benefits of intercropping mainly arise from augmenting the abundance of beneficial microorganisms and enhancing the efficiency of N use by crop plants. Discussion: This study's findings are of key importance to bolster the stability of agro-ecosystems, to guide the scientific rational use of nitrogen fertilizers, and to provide a sound theoretical basis for achieving the optimal management of intensive crop-planting patterns and green sustainable development.
RESUMEN
Crop rotation increases crop yield, improves soil health, and reduces plant disease. However, few studies were conducted on the use of intensive cropping patterns to improve the microenvironment of saline soils. The present study thoroughly evaluated the impact of a three-year maize-peanut-millet crop rotation pattern on the crop yield. The rhizosphere soil of the crop was collected at maturity to assess the effects of crop rotation on the composition and function of microbial communities in different tillage layers (0-20 cm and 20-40 cm) of sandy saline-alkaline soils. After three years of crop rotation, the maize yield and economic benefits rose by an average of 32.07% and 22.25%, respectively, while output/input grew by 10.26%. The pH of the 0-40 cm tillage layer of saline-alkaline soils decreased by 2.36%, organic matter rose by 13.44%-15.84%, and soil-available nutrients of the 0-20 cm tillage layer increased by 11.94%-69.14%. As compared to continuous cropping, crop rotation boosted soil nitrogen and phosphorus metabolism capacity by 8.61%-88.65%. Enrichment of Actinobacteria and Basidiomycota increased crop yield. Crop rotation increases microbial community richness while decreasing diversity. The increase in abundance can diminish competitive relationships between species, boost synergistic capabilities, alter bacterial and fungal community structure, and enhance microbial community function, all of which elevate crop yields. The obtained insights can contribute to achieving optimal management of intensive cultivation patterns and green sustainable development.
RESUMEN
Soil-borne diseases are exacerbated by continuous cropping and negatively impact maize health and yields. We conducted a long-term (11-year) field experiment in the black soil region of Northeast China to analyze the effects of different cropping systems on maize yield and rhizosphere soil fungal community structure and function. The experiment included three cropping systems: continuous maize cropping (CMC), maize-soybean rotation (MSR), and maize-soybean intercropping (MSI). MSI and MSR resulted in a 3.30-16.26% lower ear height coefficient and a 7.43-12.37% higher maize yield compared to CMC. The richness and diversity of rhizosphere soil fungi were 7.75-20.26% lower in MSI and MSR than in CMC. The relative abundances of Tausonia and Mortierella were associated with increased maize yield, whereas the relative abundance of Solicoccozyma was associated with decreased maize yield. MSI and MSR had higher proportions of wood saprotrophs and lower proportions of plant pathogens than CMC. Furthermore, our findings indicate that crop rotation is more effective than intercropping for enhancing maize yield and mitigating soil-borne diseases in the black soil zone of Northeast China. This study offers valuable insights for the development of sustainable agroecosystems.
RESUMEN
Soil acidification is a global environmental problem with significant impacts on agricultural production, environmental protection, and ecosystem health. Soil acidification is widespread in China, affecting crop yields, agricultural product quality, and biodiversity. Since the 1980s, much work has been done on acidic soils in China, but it is controversial whether excessive nitrogen fertilizer application can lead to soil acidification mechanisms. To address the above issues, we conducted a meta-analysis of 115 published papers to integrate and analyze the effects of N fertilizer application on soil acidification and biological properties from 1980 to 2024. We also quantified the effect of nitrogen fertilization on soil acidification and biological changes under different climatic conditions. The results showed that under long-term application of nitrogen fertilizers in China from 1980 to 2024, soil pH decreased by an average of 15.27%, and the activities of soil urease, nitrate reductase, nitrite reductase, catalase, glutamate dehydrogenase, and glutamate synthetase decreased by an average of 9.82-22.37%. The soil microbial community richness (Chao1 index) increased by 6.53%, but the community diversity (Shannon index) decreased by 15.42%. Among the dominant soil microorganisms, the relative abundance of bacteria decreased by an average of 9.67-29.38% and the abundance of gene expression of nifH, amoA-AOA, amoA-AOB, and qnorB decreased by 9.92-19.83%. In addition, we found that the mean annual temperature and rainfall impacted soil acidification via their effect on soil microbial diversity and community composition. This study provides a scientific basis for an in-depth understanding of the spatial and temporal variation of soil acidification and biological properties in China.
RESUMEN
Currently, China's soybean self-sufficiency rate is only 15%, highlighting the soybean crisis and the supply chain risks that pose a major threat to China's food security. Thus, it has become imperative to step up efforts to boost soybean production capacity while promoting the green and sustainable development of regional farmland ecosystems. In this context, the present study comprehensively investigated the effects of intercropping and nitrogen application rate on soybean yield, as well as the changes in gradients generated by different levels of nitrogen application. Based on six consecutive years of maize-soybean intercropping planting patterns, the inter-root soils of soybeans were collected at the flowering stage and evaluated for soil nitrogen content, nitrogen-assimilating enzyme activities, and microbial community composition of soybean, which were correlated with yield, to clarify the main pathways and modes of intercropping effects. The N2 level (80 kg·ha-1) was favourable for higher yield. In comparison to monocropping, the intercropping reduced yield by 9.65-13.01%, photosynthetic characteristics by 1.33-7.31%, and plant nitrogen-assimilating enzyme activities by 8.08-32.01% at the same level of N application. Likewise, soil urease and catalase activities were reduced by 9.22 and 1.80%, while soil nitrogen content declined by an average of 6.38%. Gemmatimonas and Bradyrhizobium enrichment significantly increased soil nitrogen content, photosynthetic characteristics, and soybean yield, while it was reduced by Candidatus_Udaeobacter and Candidatus_Solibacte enrichment. The results of this study provide a theoretical basis for further optimising maize-soybean intercropping, which is crucial for enhancing the agricultural production structure and improving the overall soybean production capacity.
RESUMEN
Cellulose acetate (CA) fibrous membranes blended with poly-(γ-glutamic acid) (γ-PGA) and ZnO were produced via electrospinning. The performance of the obtained composite membrane was investigated by scanning electron microscopy (SEM), Fourier transform IR spectroscopy, tensile test, water contact angle, and thermogravimetric analysis. The corn plant height, leaf area and SPAD (soil and plant analyzer development) were compared with plants covered with CA/γ-PGA and CA/γ-PGA/ZnO fibrous membranes at room temperature. Simultaneously, the water absorption and degradation rate were also studied. The results obtained indicate the potential use of these fibrous membranes for mulching film applications. The fibrous membranes could also find potential use as a material for food packing, facial mask and as antimicrobial films for wound dressing.
RESUMEN
The wild sarsaparilla (Aralia nudicaulis) plant is richly distributed in North America, mainly in Canada. In the present study, 24 extracts were obtained from the rhizome, stem, leaf and fruit of wild sarsaparilla. In the presence of RH (hexane fraction from the rhizome), the survival rate of WiDr (human colon cancer cell) was 3.5 +/- 2.7% (IC50 = 30.1 +/- 3.5 microg/ml) and that of Molt (human leukemia cell) was 2.4 +/- 2.8% (IC50 = 7.0 +/- 0.6 microg/ml). The survival rate of HELA (human cervix cancer cell) was only 1.8 +/- 0.9% in the presence of FH (hexane fraction from the fruit of wild sarsaparilla) (IC50 = 33.3 +/- 2.7 microg/ml). The cytotoxicities of RH and FH against normal human umbilical vein endothelial cells were significantly lower than against the tested human cancer cells. RH appeared to be the best extract against WiDr and Molt, whereas FH was the most effective against HELA. Because of the rich natural supply, simple extraction procedure and high yield, RH and FH of wild sarsaparilla have the potential to be developed into selective anticancer nutraceutical and/or pharmaceutical products with few side-effects and low cost.