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Silty soil has the characteristics of low natural moisture content and poor viscosity, and the strength and deformation required for foundation engineering can be satisfied by reinforcing and improving the silt. In order to study the reinforcement and improvement effects of polypropylene (PP) fiber and fly ash (FA) on cement-silty soil, an unconfined compressive strength (UCS) test, scanning electron microscope (SEM) test, and X-ray diffraction (XRD) analysis test were carried out. Cement (mixed amounts are 4%, 8%, 12%, and 16% of dry soil mass) was used as the basic modifier, and PP fiber (mixed amounts are 0%, 0.15%, 0.3%, and 0.45% of dry soil mass) compounded with FA (adding amounts of 0%, 5%, 10%, and 15% of dry soil mass) were used as an external admixture of cement-silty soil to study the mechanical properties, curing mechanism, and microstructure of the modified soil in different ages of 7 d, 14 d, 28 d, and 60 d. The test results show that with the increase in cement and curing age, the UCS of the modified soil increases, and with the increase in the PP fiber and FA, the UCS of the modified soil first increases and then decreases; there is an optimal content of FA and PP fiber, which are 10 and 0.15%, respectively. A large amount of C-S-H and AFt substances are produced inside the modified soil to cover the surface of soil particles or fill in the pores between soil particles, forming a tight spatial network structure and improving the mechanical properties of the cement-soil. The intensity of the diffraction peaks of the mineral components within the modified soils is more influenced by the cement and age, and the effect of FA is weaker. The stress-strain curve of the modified soil is divided into elastic stage, plastic deformation stage, and strain-softening stage, and the specimens in each stage have corresponding deformation characteristics. By analyzing the behavioral characteristics and curing improvement mechanism of modified soil from the duo perspective of macro-mechanical properties and microstructural composition, it can provide some basis for the engineering application of silty soil.
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Oral squamous cell carcinoma (OSCC) poses a threat to public health worldwide. LncRNA APCDD1L-AS1 has been reported to participate in tumorigenesis and development of acquired chemoresistance. However, the role of APCDD1L-AS1 in 5-fluorouracil (5-FU) resistance regulation within OSCC is still obscure. In this study, 5-FU-resistant cell models were established with OSCC cell lines (HSC-3 and HN-4). Gene expressions and protein levels were detected by RT-qPCR and Western blotting, respectively. CCK-8, colony forming, and flow cytometry were utilized to measure IC50 value, cell viability, and cell apoptosis of 5-FU-resistant OSCC cells. Dual-luciferase reporter assay and RIP assay were applied to identify the associations between miR-1224-5p and APCDD1L-AS1 or NSD2. Herein, high APCDD1L-AS1 expression was shown in OSCC tissues and cells resistant to 5-FU and related to the worse prognosis of OSCC patients. APCDD1L-AS1 knockdown impaired 5-FU resistance in 5-FU-resistant OSCC cells by reducing IC50 value, suppressing cell viability, and accelerating cell apoptosis. Besides, extracellular APCDD1L-AS1 could be transferred to sensitive cells via exosome incorporation, thereby transmitting 5-FU resistance in OSCC cells. Besides, miR-1224-5p was a molecular target of APCDD1L-AS1 and directly targeted NSD2 in 5-FU-resistant cells. MiR-1224-5p exhibited a much lower level in 5-FU-resistant tissues and increased 5-FU sensitivity in 5-FU-resistant OSCC cells. Moreover, NSD2 upregulation neutralized the influence of blocking APCDD1L-AS1 in HSC-3/5-FU and HN-4/5-FU cells on 5-FU resistance. To sum up, our study demonstrated that exosomal APCDD1L-AS1 conferred resistance to 5-FU in HSC-3/5-FU and HN-4/5-FU cells via the miR-1224-5p/NSD2 axis, thus providing a novel target for OSCC chemoresistance.
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Fluorouracilo/farmacología , MicroARNs , Neoplasias de la Boca , ARN Largo no Codificante , Carcinoma de Células Escamosas de Cabeza y Cuello , Línea Celular Tumoral , Resistencia a Antineoplásicos/efectos de los fármacos , Resistencia a Antineoplásicos/genética , Exosomas/metabolismo , Femenino , N-Metiltransferasa de Histona-Lisina/genética , N-Metiltransferasa de Histona-Lisina/metabolismo , Humanos , Masculino , MicroARNs/genética , MicroARNs/metabolismo , Persona de Mediana Edad , Neoplasias de la Boca/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/metabolismoRESUMEN
In recent years, as a result of the large-scale use of stainless steel bars in production and life, people's demand for stainless steel bars has increased. However, existing research information on stainless steel bars is scant, especially the lack of research on the mechanical properties of duplex stainless steel bars and the bonding properties of duplex stainless steel bars to concrete. Therefore, this paper selects 177 duplex stainless steel bars with different diameters for room temperature tensile test, and then uses mathematical methods to provide suggestions for the values of their mechanical properties. The test results show that the duplex stainless steel bar has a relatively high tensile strength of 739 MPa, no significant yield phase, and a relatively low modulus of elasticity of 1.43 × 105 MPa. In addition, 33 specimens were designed to study the bonding properties of duplex stainless steel bars to concrete. In this paper, the effects of concrete strength, duplex stainless steel reinforcement diameter, the ratio of concrete cover to reinforcing steel diameter, and relative anchorage length on the bond stress were investigated, and a regression model was established based on the experimental results. The results show that, with the concrete strength concrete strength from C25 to C40, the compressive strength of concrete increased by 56.1%, the bond stress increased by 27%; the relative anchorage length has been increased from 3 to 6, the relative anchorage length has doubled, and the bond stress has increased by 13%; and, the ratio of concrete cover to reinforcing steel diameter increased to a certain range on the bond stress has no significant effect and duplex stainless steel reinforcement diameter has little effect on the bond stress. The ratio of concrete cover to reinforcing steel diameter from 3.3 to 4.5 and the bond stress increased by 24.7%. A ratio of concrete cover to reinforcing steel diameter greater than 4.5 has no significant effect on the bond stress, with the average bond stress value of 20.1 MPa. The duplex stainless steel bar diameter has little effect on the bond stress for the diameters of 12 mm, 16 mm, 25 mm duplex stainless steel bar, and their average bond stress is 19.9 MPa.
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With the acceleration of infrastructure construction in various countries, more and more highway tunnels have been built. As a permanent structure to maintain the long-term stability and durability of the tunnel, the tunnel lining structure (TLS) is prone to durability damage in the later operation process, which affects the safety of traffic and the whole loading capacity of the tunnel, so it is very important to evaluate the durability of the TLS. Nowadays, the TLS durability evaluation methods ignore the ambiguity and randomness of the lining structure (LS) durability evaluation index, which have certain limitations. In order to evaluate the durability of highway TLS scientifically and rationally, this paper proposes a method for evaluating the durability of highway TLS based on the matter-element extension (MEE)- simple correlation function (SCF) method- cloud model (CM). A case study was carried out by combining the advantages of the above three methods, a LS durability evaluation model was established, and based on the relevant data from the actual inspection of a highway tunnel lining durability disease in China, the model was used to evaluate the durability of the lining of this highway tunnel, and the durability grades were equivalently divided into five grades: in good shape(I), slightly damaged(II), medium damaged(III), severely damaged(IV), extremely dangerous(V). The result show that this tunnel lining belongs to IV, and the result of this evaluation method is in accordance with the actual damage condition of the project, and the accuracy reaches 92.75%. At the same time, a comparative study was carried out in combination with the AHP-Extenics to verify the reasonableness and feasibility of this method. This study provides a new method for durability evaluation of LS, offers a theory basis for judging the durability of highway TLS, and lays a foundation for subsequent maintenance and prevention.
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Industria de la Construcción , Materiales de Construcción , ChinaRESUMEN
In this study, the effect of adding nano-silica (NS) particles on the properties of concrete containing coal fly ash were explored, including the mechanical properties, impact resistance, chloride penetration resistance, and freezing-thawing resistance. The NS particles were added into the concrete at 1%, 2%, 3%, 4%, and 5% of the binder weight. The behavior under an impact load was measured using a drop weight impact method, and the number of blows and impact energy difference was used to assess the impact resistance of the specimens. The durability of the concrete includes its chloride penetration and freezing-thawing resistance; these were calculated based on the chloride diffusion coefficient and relative dynamic elastic modulus (RDEM) of the samples after the freezing-thawing cycles, respectively. The experimental results showed that the addition of NS can considerably improve the mechanical properties of concrete, along with its freezing-thawing resistance and chloride penetration resistance. When NS particles were added at different replacement levels, the compressive, flexural, and splitting tensile strengths of the specimens were increased by 15.5%, 27.3%, and 19%, respectively, as compared with a control concrete. The addition of NS enhanced the impact resistance of the concrete, although the brittleness characteristics of the concrete did not change. When the content of the NS particles was 2%, the number of first crack impacts reached a maximum of 37, 23.3% higher compared with the control concrete. Simultaneously, the chloride penetration resistance and freezing-thawing resistance of the samples increased dramatically. The optimal level of cement replacement by NS in concrete for achieving the best impact resistance and durability was 2-3 wt%. It was found that when the percentage of the NS in the cement paste was excessively high, the improvement from adding NS to the properties of the concrete were reduced, and could even lead to negative impacts on the impact resistance and durability of the concrete.
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BACKGROUND: LncRNA NEAT1 promotes inflammatory responses, which contribute to recurrent aphthous stomatitis (RAS). This study focused on the involvement of NEAT1 in RAS. METHODS: RT-qPCR and ELISA were performed to determine the expression of NEAT1 and proinflammatory factors (IL-2, IL-1ß, and TNF-α) in plasma from patients with a history of RAS and showing symptom (n = 80, S-RAS group), people with a history of RAS but showing no symptom (n = 80, NS-RAS group), and controls without a history of RAS (n = 80, Control group). Correlation analysis was performed with Pearson's correlation coefficient. S-RAS group received treatmen,t and plasma levels of NEAT1 and proinflammatory factors were compared before and after treatment. S-RAS group was followed up for 12 months, and the recurrence was recorded. RESULTS: Plasma NEAT1, IL-2, IL-1ß, and TNF-α levels were the highest in the S-RAS group, followed in turn by NS-RAS and control groups. NEAT1 was positively and significantly correlated with IL-2, IL-1ß, and TNF-α across S-RAS and NS-RAS samples, but not control samples. After treatment, plasma levels of NEAT1, IL-2, IL-1ß, and TNF-α decreased significantly. Moreover, a higher recurrence rate was observed during the follow-up in patients with high plasma NEAT1 levels. CONCLUSION: NEAT1 is upregulated in RAS and correlated with multiple proinflammatory factors. Moreover, NEAT1 has predictive values for RAS.
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ARN Largo no Codificante , Estomatitis Aftosa , Humanos , Estomatitis Aftosa/genética , Factor de Necrosis Tumoral alfa/genética , Regulación hacia ArribaRESUMEN
An experimental study was conducted to investigate the effect ofnano-SiO2 and steel fiber content on the durability of concrete. Five different dosages of nano-SiO2 particles and five volume dosages of steel fiber were used. The durability of concretes includes permeability resistance, cracking resistance, carbonation resistance, and freezing-thawing resistance, and these were evaluated by the water permeation depth, number of cracks, total cracking area per unit area of the specimens, carbonation depth of the specimens, and the relative dynamic elastic modulus of the specimens after freezing-thawing cycles, respectively. The results indicate that the addition of nano-SiO2 particles significantly improves the durability of concrete when the content of nano-SiO2 is limited within a certain range. With the increase of nano-SiO2 content, the durability of concrete first increases and then decreases. An excessive number of nano-SiO2 particles could have an adverse effect on the durability of the concrete. The addition of the correct amount of steel fibers improves the carbonation resistance of concrete containing nano-particles, but excessive steel fiber reduces the carbonation resistance. Moreover, the addition of steel fibers reduces the permeability resistance of concrete containing nano-particles. The incorporation of steel fiber enhanced the freezing-thawing resistance and cracking resistance of concrete containing nano-particles. With increasing steel fiber content, the freezing-thawing resistance of the concrete containing nano-particles increases, and the cracking resistance of the concrete decreases gradually.
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Acute pancreatitis (AP) is the acute inflammation of the pancreas. The morbidity of AP has increased in recent years. Certain patients eventually develop severe AP (SAP), which rapidly progresses to multiple organ dysfunction; the incidence of this occurring in patients with AP is 20-30%. To date, no specific drugs or methods exist to treat this disease. Rutaecarpine relaxes vascular smooth muscle by stimulating calcitonin gene-related peptide (CGRP) release via activation of vanilloid receptor subtype 1 (VR1). It has been demonstrated that rutaecarpine induces a therapeutic effect on SAP. The present study was conducted to characterize the molecular mechanisms underlying the protective effects of rutaecarpine against AP using a rat model of AP. Gross pathological changes of the pancreas, as well as the pancreatic tissue histopathological score, were assessed following treatment with rutaecarpine, capsazepine or a combination of the two. Serum amylase activity was detected using an automatic biochemistry analyzer. Changes in the serum concentrations of interleukin (IL)-6, tumor necrosis factor (TNF-α), IL-10 and CGRP were assessed by ELISA and radioimmunoassay. The results demonstrated that pre-treatment with rutaecarpine markedly decreased pancreatic inflammation and necrosis, reduced the volume of ascites, and significantly increased the plasma concentration of CGRP and the serum concentration of IL-10, an anti-inflammatory cytokine. However, serum concentrations of the inflammatory cytokines IL-6 and TNF-α were decreased. The effect of rutaecarpine treatment markedly improved with increases in the drug dose. Capsazepine, as a competitive vanilloid receptor antagonist, abolished these protective effects of rutaecarpine against AP. Therefore, the results of the present study indicate that rutaecarpine protects against AP in rats by upregulating endogenous CGRP release via activation VR1 of, to improving the microcirculation of the pancreatic tissue and regulate the expression of inflammatory factors.
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PURPOSE: miRNAs are stable and can be extracted from tissues, blood and other body fluid without degradation. miRNAs are abnormally expressed in the presence of a pathological status, including cancer. Therefore, miRNAs are ideal biomarkers for cancer diagnosis and prognosis. Patients with triple negative breast cancer (TNBC) suffer the worst prognosis, although great efforts have been made. Many studies have investigated the role of miRNAs in predicting the outcomes of TNBC patients for better adjustment of treatment. However, results were inconsistent. Thus, we performed a meta-analysis to summarize the published studies for conclusive results. METHODS: Eligible studies from different database were retrieved from the online databases, and we used STSTA 12.0 to analysis the prognostic role of miRNAs in triple negative breast cancer. RESULTS: Overall high miRNA expression indicated a worse survival with HR value of 1.78 (95% CI: 0.97-3.25). However, subtotal HRs of oncogenic miRNAs and tumor suppressive miRNAs were 2.73 (95% CI: 2.08-3.57; P<0.001) and 0.44 (95% CI: 0.21-0.90; P = 0.024), respectively, and no heterogeneity was observed within the subgroups. CONCLUSIONS: The miRNAs showed a slightly stronger prognostic value for disease-free survival, relapse-free survival and distant metastasis-free survival compared to the overall survival of TNBC patients. Circulating miRNAs could serve as potential biomarkers for the prognosis of TNBC patients and need further investigation.
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Biomarcadores de Tumor/genética , Regulación Neoplásica de la Expresión Génica , MicroARNs/genética , Neoplasias de la Mama Triple Negativas/genética , Femenino , Humanos , Pronóstico , Neoplasias de la Mama Triple Negativas/patología , Neoplasias de la Mama Triple Negativas/terapiaRESUMEN
Sensory nerves are a kind of nerve that conduct afferent impulses from the periphery receptors to the central nervous system (CNS) and are able to release neuromediators from the activated peripheral endings. Sensory nerves are particularly important for microcirculatory response, and stimulation of pancreatic sensory nerves releases a variety of neuropeptides such as substance P (SP), calcitonin gene-related peptide (CGRP), etc., leading to neurogenic inflammation characterized as the local vasodilatation and plasma extravasation. Deactivation of sensory nerves often leads to the disturbances of pancreatic microcirculation. Pancreatitis is a common digestive disease that can lead to severe complications and even death if it goes untreated. Experimental studies in animals and tissue analysis in patients with pancreatitis have shown significant changes in sensory nerves supplying the pancreatic gland. Thus making clear the whole mechanism of pancreatitis is essential to treat and cure it. Sensory nerves may have a close correlation with the development of pancreatitis, and knowing more about the role of sensory nerve in pancreatitis is important for the treatment for pancreatitis. This review is aimed to summarize the relationship between sensory nerves and pancreatitis.
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This paper aims to clarify the effect of steel fiber on the flexural toughness of the high performance concrete containing fly ash and nano-SiO2. The flexural toughness was evaluated by two methods, which are based on ASTM C1018 and DBV-1998, respectively. By means of three-point bending method, the flexural toughness indices, variation coefficients of bearing capacity, deformation energy, and equivalent flexural strength of the specimen were measured, respectively, and the relational curves between the vertical load and the midspan deflection (P(V)-δ) were obtained. The results indicate that steel fiber has great effect on the flexural toughness parameters and relational curves (P(V)-δ) of the three-point bending beam specimen. When the content of steel fiber increases from 0.5% to 2%, the flexural toughness parameters increase gradually and the curves are becoming plumper and plumper with the increase of steel fiber content, respectively. However these flexural toughness parameters begin to decrease and the curves become thinner and thinner after the steel fiber content exceeds 2%. It seems that the contribution of steel fiber to the improvement of flexural toughness of the high performance concrete containing fly ash and nano-SiO2 is well performed only when the steel fiber content is less than 2%.
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Ceniza del Carbón/normas , Materiales de Construcción/normas , Dióxido de Silicio/normas , Acero/normas , Ensayo de Materiales/métodos , Nanoestructuras/normas , Resistencia a la TracciónRESUMEN
This study aimed at evaluating the short-term efficacy and safety of probiotics as an aid in the treatment of Candida-associated stomatitis in a randomised controlled trial. A total of 65 patients were randomly assigned to receive oral local antifungal agents alone (gargle 2% sodium bicarbonate solution for 30 s, wait 10 min and then apply 2% nystatin paste) or these agents plus local probiotics (the mixture of Bifidobacterium longum, Lactobacillus bulgaricus and Streptococcus thermophilus) three times per day for 4 weeks. Parameters related to hyperaemia, visual analogue scale scores, culture of resting saliva and a lingual dorsum swab and adverse reactions were assessed or recorded in the beginning, middle and end of treatment. Although the baseline characteristics of the participants were similar, both groups showed a significant reduction in pain level and hyperaemia on the tongue mucosa (P = 0.000) after 4-week application. However, despite the reduction in hyperaemia in the probiotic group, these improvements did not display statistically significant differences. The detection rate of Candida spp. was 100% before treatment and 8.21% in the experimental group and 34.6% in the control group after treatment. The detection rate of Candida spp. decreased (P = 0.000) in both groups and was significantly lower in the probiotic group than the control group (P = 0.038). Other analysed micro-organisms, including the decreased detection rate for Lactobacillus spp. (P = 0.049) and the increased detection rate for Staphylococcus epidermidis (P = 0.019), did not display consistent change trends in the probiotics group. Compared with conventional antifungal therapies for oral candidiasis, the inclusion of locally administered probiotics helped improve certain clinical conditions and reduced the prevalence of Candida spp., although the impact of probiotics on oral bacterial species remains to be further studied.
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Candidiasis Bucal/terapia , Probióticos/administración & dosificación , Anciano , Antifúngicos/uso terapéutico , Bifidobacterium , Candida/aislamiento & purificación , Femenino , Humanos , Lactobacillus , Masculino , Persona de Mediana Edad , Nistatina/uso terapéutico , Bicarbonato de Sodio/uso terapéutico , Streptococcus thermophilusRESUMEN
AIM: To investigate the antifibrotic effects of bone morphogenetic protein-7 (BMP-7) on Schistosoma japonicum (S. japonicum)-induced hepatic fibrosis in BALB/C mice. METHODS: Sixty BALB/C mice were randomly divided into three groups, including a control group (group A, n = 20), model group (group B, n = 20) and BMP-7 treated group (group C, n = 20). The mice in group B and group C were abdominally infected with S. japonicum cercariae to induce a schistosomal hepatic fibrosis model. The mice in group C were administered human recombinant BMP-7. Liver samples were extracted from mice sacrificed at 9 and 15 wk after modeling. Hepatic histopathological changes were assessed using Masson's staining. Transforming growth factor-beta 1 (TGF-ß1), alpha-smooth muscle actin (α-SMA), phosphorylated Smad2/3 (pSmad2/3) and Smad7 protein levels and localization were measured by Western blotting and immunohistochemistry, respectively, and their mRNA expressions were detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The schistosomal hepatic fibrosis mouse model was successfully established, as the livers of mice in group B and group C showed varying degrees of typical schistosomal hepatopathologic changes such as egg granuloma and collagen deposition. The degree of collagen deposition in group C was higher than that in group A (week 9: 22.95 ± 6.66 vs 2.02 ± 0.76; week 15: 12.84 ± 4.36 vs 1.74 ± 0.80; P < 0.05), but significantly lower than that in group B (week 9: 22.95 ± 6.66 vs 34.43 ± 6.96; week 15: 12.84 ± 4.36 vs 18.90 ± 5.07; P < 0.05) at both time points. According to immunohistochemistry data, the expressions of α-SMA, TGF-ß1 and pSmad2/3 protein in group C were higher than those in group A (α-SMA: week 9: 21.24 ± 5.73 vs 0.33 ± 0.20; week 15: 12.42 ± 4.88 vs 0.34 ± 0.27; TGF-ß1: week 9: 37.00 ± 13.74 vs 3.73 ± 2.14; week 15: 16.71 ± 9.80 vs 3.08 ± 2.35; pSmad2/3: week 9: 12.92 ± 4.81 vs 0.83 ± 0.48; week 15: 7.87 ± 4.09 vs 0.90 ± 0.45; P < 0.05), but significantly lower than those in group B (α-SMA: week 9: 21.24 ± 5.73 vs 34.39 ± 5.74; week 15: 12.42 ± 4.88 vs 25.90 ± 7.01; TGF-ß1: week 9: 37.00 ± 13.74 vs 55.66 ± 14.88; week 15: 16.71 ± 9.80 vs 37.10 ± 12.51; pSmad2/3: week 9: 12.92 ± 4.81 vs 19.41 ± 6.87; week 15: 7.87 ± 4.09 vs 13.00 ± 4.98; P < 0.05) at both time points; the expression of Smad7 protein in group B was higher than that in group A and group C at week 9 (8.46 ± 3.95 vs 1.00 ± 0.40 and 8.46 ± 3.95 vs 0.77 ± 0.42; P < 0.05), while there were no differences in Smad7 expression between the three groups at week 15 (1.09 ± 0.38 vs 0.97 ± 0.42 vs 0.89 ± 0.39; P > 0.05). Although minor discrepancies were observed, the results of RT-PCR and Western blotting were mainly consistent with the immunohistochemical results. CONCLUSION: Exogenous BMP-7 significantly decreased the degree of hepatic fibrosis in both the acute and chronic stages of hepato-schistosomiasis, and the regulatory mechanism may involve the TGF-ß/Smad signaling pathway.
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Proteína Morfogenética Ósea 7/administración & dosificación , Cirrosis Hepática/prevención & control , Hígado/efectos de los fármacos , Schistosoma japonicum/patogenicidad , Esquistosomiasis Japónica/tratamiento farmacológico , Proteínas Smad/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Animales , Modelos Animales de Enfermedad , Femenino , Regulación de la Expresión Génica , Humanos , Hígado/metabolismo , Hígado/patología , Cirrosis Hepática/genética , Cirrosis Hepática/metabolismo , Cirrosis Hepática/parasitología , Cirrosis Hepática/patología , Ratones , Ratones Endogámicos BALB C , ARN Mensajero/metabolismo , Proteínas Recombinantes/administración & dosificación , Esquistosomiasis Japónica/genética , Esquistosomiasis Japónica/metabolismo , Esquistosomiasis Japónica/parasitología , Esquistosomiasis Japónica/patología , Transducción de Señal/efectos de los fármacos , Proteínas Smad/genética , Proteína Smad2/metabolismo , Proteína smad3/metabolismo , Proteína smad7/metabolismo , Factores de Tiempo , Factor de Crecimiento Transformador beta1/genéticaRESUMEN
RNA binding motif 5 (RBM5) is a tumor suppressor gene that regulates cell proliferation, differentiation and apoptosis through pre-mRNA splicing of related genes. This study aimed to detect RBM5 and KRAS expression in pancreatic ductal adenocarcinoma and their association with clinicopathological features. Detection of RBM5 and KRAS expression by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting was performed at mRNA and protein levels, respectively, in pancreatic cancer and non-tumor tissues. In addition, the association of RBM5 and KRAS expression with clinicopathological parameters and tumor recurrence was analyzed. The expression of RBM5 was significantly downregulated in pancreatic cancer tissues compared to peritumoral tissues at the mRNA and protein levels. Contrastingly, KRAS was significantly overexpressed in pancreatic cancerous tissues compared to peritumoral tissues. Analysis revealed that RBM5 expression was negatively correlated with KRAS expression in pancreatic cancer. Furthermore, reduced RBM5 expression has a close association with lymph node metastasis, distant metastasis, Union for International Cancer Control (UICC) stage and nerve and venous invasion, while overexpression of KRAS proteins was significantly correlated with tumor size, lymph node metastasis, UICC stage and nerve and venous invasion of pancreatic cancer. Significant RBM5 underexpression and KRAS overexpression were observed in pancreatic cancer compared to non-tumor tissues. There is a close association of differential RBM5 and KRAS with poor clinicopathological features, suggesting their potential roles in the progression and metastasis of pancreatic cancer.
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The incidence of pancreatic carcinoma, a gastrointestinal malignancy, is on the increase and effective therapeutic strategies are therefore required. This study aimed to construct a recombinant plasmid pcDNA3.1(-) shCEACAM6-yCDglyTK from CEACAM6 targeting shRNA and the fusion suicide gene yCDglyTK for inhibition of SW1990 human pancreatic carcinoma cell growth and invasion. A plasmid containing hU6 promoter and CEACAM6 targeting short hairpin RNA (CEACAM6-shRNA) frame was constructed. It was subcloned to a CEA promoter-driven fusion suicide gene pcDNA3.1(-)yCDglyTK. The recombinant plasmid pcDNA3.1(-) shCEACAM6-yCDglyTK was identified by restriction endonuclease analysis and DNA sequencing. The recombinant plasmid was delivered into SW1990 human pancreatic carcinoma cells, the mRNA and protein expression of yCDglyTK and CEACAM6 was examined by RT-PCR, western blot analysis and immunofluorescence. SW1990 cells were treated with the prodrug 5-fluorocytosine (5-FC), and the cell viability was evaluated using the 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The invasiveness and migration of SW1990 cells were evaluated by transwell migration assays. The restriction endonuclease analysis and DNA sequencing confirmed the construction of the recombinant plasmid pcDNA3.1(-) shCEACAM6-yCDglyTK. Reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis outcomes showed that yCDglyTK was expressed in SW1990 cells and expression of CEACAM6 in SW1990 cells was significantly knocked down. MTT assay showed that the mean viability of SW1990 cells was significantly reduced after administration of the prodrug 5-FC in vitro. Transwell migration assays showed that invasion and migration action of SW1990 cells was significantly inhibited. In conclusion, recombinant plasmid pcDNA3.1(-) shCEACAM6-yCDglyTK was successfully constructed. The recombinant plasmid may therefore serve as a novel gene therapy approach for pancreatic carcinoma.
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OBJECTIVE: The determination method of histatins 5 in human saliva with reversed-phase high performance liquid chromatography (HPLC) was developed. METHODS: Salivary samples were collected and diluted with phosphate buffer (pH 2.5). The upper solution was determined with HPLC. Phosphate buffer (pH 3.5) of the mobile phase and C18 column was used throughout the experiment. The detection wavelength was 276 nm. RESULTS: The linear ranges were 1.0-50.0 microg x mL(-1). The detection limit was 0.12 microg x mL(-1). The relative standard derivations (RSD) of standard solution for reserved time and peak area were 0.68% and 4.13% respectively. The proposed method was applied for analysis of salivary samples and the satisfactory results were obtained. RSD for sample determination was 4.41% and the average recoveries were 88.4%-109.0%. CONCLUSION: The method was quick, simple and accurate. Analytical time was less than 15 min. It was adapted for analysis of salivary histatins 5 in salivary samples.
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Cromatografía Líquida de Alta Presión , Histatinas , Humanos , SalivaRESUMEN
Herpes associated erythema multiforme (HAEM) is an acute exudative dermatic and mucosal disease caused by infecting herpes simplex virus. It has recurrence and idiorestriction, characterized by increasing of CD4+T leukomonocyte. This article reports a case of herpes associated erythema multiforme, and by way of reviewing relevant literature, discusses the possible mechanism, diagnosis, treatment and prognosis of HAEM.
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Eritema Multiforme , Herpes Simple , Humanos , Recurrencia , SimplexvirusRESUMEN
ZnO thin film is a promising material for short-wave laser and LED etc, due to its high excition binding energy, intense stimulated emission, low lasing threshold, and high working temperature. ZnO thin films were prepared by laser molecular beam epitaxy (L-MBE) in our work. At room-temperature we reported the measurements of absorption spectra and emission spectra of ZnO thin films excited by various optical pumping intensities. High structural perfection of our sample was shown in these figures. We studied the properties and mechanism of stimulated emission in ZnO thin films. The relation between emission intensity and pumping intensity was obtained. Time behaviors of the stimulated emission under relatively high pumping intensity, spontaneous emission, and laser pulses were compared, and hence the stimulated emission of ZnO thin films was proved.