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Comment on "The effect of different nerve block strategies on the quality of post-operative recovery in breast cancer patients: A randomized controlled study" by Wang and colleagues.

Li, Qi-Pei; Li, Xin-Tao; Xue, Fu-Shan.

Eur J Pain ; 28(1): 176-177, 2024 01.

Artículo en Inglés | MEDLINE | ID: mdl-37750806

Asunto(s)

Neoplasias de la Mama , Bloqueo Nervioso , Humanos , Femenino , Neoplasias de la Mama/cirugía , Anestésicos Locales , Proyectos de Investigación

[The effect of short hairpin RNA on hTERT expression].

Guo, Ying; Song, Tian-bao; Wu, Jing; Zhao, Yan; Li, Qi-pei.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 21(3): 382-3, 2005 May.

Artículo en Chino | MEDLINE | ID: mdl-15862164

RESUMEN

AIM: To study the effect of short hairpin RNA (shRNA) on hTERT expression. METHODS: Oligonucleotides encoding shRNA against hTERT was cloned into a mammalian shRNA expression vector pUC18U6 to form pUC18U6ht which was transfected into HepG2 cells by using liposome. HepG2 cells transfected by pUC18U6 and pUC18U6GFPsir which expressed shRNA against green fluorescent protein were used as controls. hTERT mRNA in the transfected cells was quantified by using real-time fluorescent RT-PCR. RESULTS: Compared with the controls, the short hairpin RNA against hTERT decreased the hTERT mRNA level by 49% (P<0.05). CONCLUSION: hTERT expression was reduced by the shRNA.

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Regulación de la Expresión Génica/genética , Secuencias Invertidas Repetidas , ARN/química , ARN/genética , Telomerasa/genética , Animales , Secuencia de Bases , Línea Celular Tumoral , Humanos , Conformación de Ácido Nucleico , ARN Mensajero/genética , ARN Mensajero/metabolismo

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