RESUMEN
OBJECTIVE: To investigate the disparity in dental caries between native and migrant children in Shanghai, China. METHODS: Between 2013 and 2015, a random cluster sample of native and migrant children aged 5, 9, 12 and 15 years was collected from each district in Shanghai. Oral examination was performed following the World Health Organization (WHO) method, and findings were reported as decayed-missing-filled teeth of primary dentition (dmft) and permanent dentition (DMFT). RESULTS: A total of 10 150 children were examined, and 33.6% of them were migrants. Migrant children had a higher prevalence of deciduous caries than native children (the 5-year-old age group, 67.8% vs 63.0%, P = 0.024; the 9-year-old age group, 75.9% vs 66.1%, P < 0.001), and higher dmft values were found in migrant children. But with respect to permanent teeth, no statistical differences were found between the two groups in caries prevalence or DMFT. After controlling for potential confounders by logistic regression, migrant children showed a higher risk of deciduous caries (odds ratio 1.42, 95% confidence interval 1.25-1.61, P < 0.001) but not of permanent caries. Migrant children exhibited relatively lower deciduous Restorative Care Index (RCI). However, 9- and 15-year-old migrant children had a higher permanent RCI than their native counterparts. CONCLUSIONS: Dental caries prevalence in migrant children was higher in the deciduous teeth but not in the permanent teeth compared to that in their native counterparts. School-based dental public health services may contribute to reducing the disparity in dental health status between migrant and native children.
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Caries Dental , Migrantes , Adolescente , Niño , Preescolar , China , Estudios Transversales , Índice CPO , Humanos , PrevalenciaRESUMEN
PURPOSE: To obtain the clone of Streptococcus salivarius 57.I urease genes ureIABCEFGD and investigate the relationship between ureolytic activity expression of this clone in Escherichia coli and nickel ions. METHODS: The target gene was cloned by polymerase chain reaction in 2 parts separately. Then, 2 plasmids were digested by specific restriction enzymes and ligated together. The obtained plasmids were subjected to nucleotide sequence analysis and transformed into E.coli TG-1. The recombinant E.coli was added without or with different level of NiCl2. The amount of ammonia generated by ureolytic activity of each sample was measured by Nessler's assay. SPSS 17.0 software package was used for correlation analysis. RESULTS: The clone of urease genes ureIABCEFGD was proved by sequence analysis and BLAST search. The amount of ammonia generated by the recombinant strain had a positive correlation with the level of NiCl2(r=0.9714,P<0.01). When the level of NiCl2 was 50 µmol/L, the amount of ammonia reached maximum and would have little variance despite the increase of NiCl2 level. CONCLUSIONS: Ureolytic activity expression of ureIABCEFGD has a positive correlation with the level of added NiCl2 not exceeding 50 µmol/L. The present clone can be used to further investigate the regulation of the ureolytic activity expression of Streptococcus salivarius urease gene. Supported by Natural Science Foundation of Shanghai Municipality(08ZR1416800) and Research Fund of Science and Technology Committee of Shanghai Municipality(11411950900).
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Escherichia coli , Ureasa , Iones , Níquel , Plásmidos , StreptococcusRESUMEN
PURPOSE: To analyze the status of 5-year-old children's oral caries in Shanghai from 2008 to 2011, and to provide information to evaluate oral health status and improve service level. METHODS: By using equal-sized randomly sampling method, this survey was carried out on the caries status of randomly selected 950 children aged 5 years in Shanghai respectively in 2008 and 2009, and 900 children respectively in 2010 and 2011. The data of prevalence of dental caries, dmft, caries filling constituent ratio and SiC index were collected. Statistical analysis was performed using SPSS 13.0 software package by means of independent-samples t test, one way ANOVA and Chi-square test. RESULTS: From 2008 to 2011, the prevalence of deciduous tooth caries among 5-year-old children was 63.47%, 64.00%, 64.89% and 64.44%, respectively. The mean dmft score was 2.96, 2.99, 3.23 and 3.09, respectively. The caries filling ratio was 10.93%, 12.76%, 16.96% and 20.63%, respectively. The SiC index was 7.10, 7.20, 7.74 and 7.37, respectively. The prevalence of caries and mean dmft showed no significant difference in four years(P>0.05), and the caries filling constituent ratio was remarkably increased (P<0.01). The SiC index was two times higher than dmft. Each year the prevalence of deciduous tooth caries and mean dmft in urban areas were lower than in suburban areas (P<0.01), but the children came from urban areas showed significant higher filling rate than those from suburban areas (P<0.01). Deciduous tooth caries were concentrated in one-third of children. CONCLUSIONS: The prevalence of deciduous tooth caries and dmft of 5-year-old children in Shanghai changed little, and the caries filling constituent ratio has increased year by year. To promote children's oral health further, we still need to take more active preventive measures for high caries risk group.
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Índice CPO , Caries Dental , Preescolar , China , Humanos , Boca , Prevalencia , Diente PrimarioRESUMEN
PURPOSE: The prosthetic status and associated factors of the elders in Shanghai were evaluated to provide references for the government to issue related policies. METHODS: The multi-stage stratified and isometric random sampling was used.The information of tooth loss and prosthodontic restoration was collected by oral examination in 788 elder residents aged between 65 to 74 years in Shanghai, and 325 subjects was randomly drawn from the given group to collect the information concerning socioeconomic factors and life health situation by means of interviewer administered questionnaire. A Logistic regression model(Stata.7.0) was used to analyze the related factors that have effects on whether or not those elders would choose to do the restoration. RESULTS: The restoration rate of the patient was 61.73%. The restoration rate of the patients with the defect of dentition was significantly lower than that of the edentulous(P<0.01).The constituent ratio of the denture restoration with fixed denture, the removable denture, the fixed denture and removable denture, the full denture, the informal fixed bridge was 21.94%, 41.58%, 5.87%, 20.92%, 9.69%, respectively. The ratio of full denture, fixed denture and informal fixed bridge of the suburb elders was significantly higher than that of the urban ones(P<0.01). Logistic regression analysis revealed that the position of teeth defect, the gender and the number of missing teeth were significant predictors of denture or non-denture wearers. CONCLUSIONS: Ratio of denture wearers is low in the older individuals with missing teeth in Shanghai. It is suggested that the government should take effective efforts to strengthen the people's oral health consciousness so that the elder patients could restore their lost teeth in time and more effectively. Supported by Research Project of Shanghai Municipal Health Bureau (Grant No.2007009).
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Dentaduras , Pérdida de Diente , Anciano , China , Dentadura Parcial Fija , Humanos , Salud Bucal , DienteRESUMEN
PURPOSE: To investigate the condition of teeth defect and denture restoration among the middle aged and elder people in Shanghai and to analyse the variation tendency so as to provide relevant policy basis for the government. METHODS: Multistage stratification isasteric random sample was used to acquire the teeth defect and denture restoration information among 791 people from 35 to 44 and 788 people from 65 to 74 years old, respectively. SPSS13.0 software package was used for data analysis. RESULTS: The teeth defect rate of the middle aged and elder people in Shanghai was 21.0% and 81.0%, respectively. The mean permanent teeth number was 28.9 and 19.5. The edentulous rate of the elder people was 10.8%. The defect rate was highest in the molar area, and lowest in the canine agra. The denture restoration rate of the middle aged and elder people was 59.6% and 61.7%, respectively. The rate of the removable denture restoration was higher than that of the fixed denture. The rate of full denture, fixed denture and informal fixed bridge restoration in the suburb area was higher than that in the urban area(P<0.01). CONCLUSIONS: The condition of teeth defect and denture restoration among middle aged and elder people in Shanghai is still unsatisfactory even though it has been getting better in the last decade. It is suggested that the government take efforts to lessen the teeth defect by control of caries and periodontal disease, and to improve the restoration level of the middle aged and elder people.
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Dentadura Parcial Fija , Dentadura Parcial Removible , Anciano , China , Caries Dental , Humanos , Persona de Mediana Edad , Diente Molar , Enfermedades Periodontales , DienteRESUMEN
OBJECTIVE: The aim of this study was to observe the change of the prevalence of five-year-old children's dental caries for 10 years in Shanghai and to establish a related policy to provide a basis for the government. METHODS: The sampling method was multi-stage, stratified, adqulis capacity and whole flock in this study. The method of third national survey for oral health was used. The data of prevalence of dental caries, dmft and relative component were collected and were compared with the data of the second survey in 1995. RESULTS: The prevalence of dental caries, dmft, caries filling constitutent ratio of 5-year-old children in 2005 was 71.74%, 4.17, 7.70%, respectively, and denscend 6.54%, 0.59, 1.12% compare with the data in 1995. There was significant difference between them (P<0.01). CONCLUSION: The results showed the descend trend of prevalence of dental caries in 5-year-old children in Shanghai and the oral health condition had an obvious improvement. However, caries filling constitutent ratio was decreases. Therefore, it is very important to strengthen the public oral health care service for children.
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Índice CPO , Caries Dental , Niño , Preescolar , China , Femenino , Humanos , Masculino , Salud Bucal , Prevalencia , Diente PrimarioRESUMEN
PURPOSE: To observe the 3 years changes of deciduous teeth caries in 372 children aged 3 years old in Shanghai. METHODS: 372 samples of 3 year-old children from kindergartens in Shanghai were selected for clinical examination.The prevalence of dental caries,mean dmft score, caries severity index(CSI) and caries filling rate were calculated. The results were analyzed with SPSS13.0 software package for Chi(2) test, ANOVA and SNK. RESULTS: It was found that from 2005 to 2007,the prevalence of dental caries was 40.90%, 50.80% and 62.40%, respectively. The mean dmft was 2.17+/-3.38+/-2.56+/-3.61 and 3.15+/-3.87, respectively. The CSI was 6.38+/-11.16+/-7.67+/-12.04 and 8.73+/-13.03, respectively. The caries filling rate was 5.35%,13.46% and 18.15%,respectively. The prevalence of dental caries, mean dmft, CSI and caries filling rate increased gradually as the children grew up. There was significant difference among 3 years (Chi(2)=16.423,P<0.01;F=8.185,P<0.01;F=3.509,P<0.05;Chi(2)=16.423,P<0.05). SNK analysis showed that there was significant difference of dmft between 2005 and 2006 ,2005 and 2007 was found. Significant difference of CSI only between 2005 and 2007. No significant difference of incidence of caries between 2006 and 2007 was found. There was no significant difference between male and female in prevalence of dental caries,mean dmft,CSI and caries filling rate (P>0.05). CONCLUSION: The trend that the status of dental caries rapidly increases as the children grow up is decreasing in Shanghai;the filling rate is still very low, early prevention and treatment for deciduous caries are needed. Supported by Shanghai Municipal Health Bureau for Development of Science and Technology (Grant No.044034).