RESUMEN
Chinese sturgeon (Acipenser sinensis) with an evolutionary history of over 200 million years, has a long lifespan, and an extremely late and asynchronous sexual maturation (8-18 years for males and 14-26 years for females), resulting in the difficulty of mature adult culture. However, little is known about the regulatory mechanisms of the transition among ovarian maturation stages in the Chinese sturgeon. We performed de novo transcriptome sequencing of the Chinese sturgeon at different ovarian maturation stages (FII, FIII, and FIV). The number of differentially expressed genes (DEGs) between FII and FIII/FIV (33,517/34,217) was more than that between FIII and FIV (22,123), suggesting that the transition from FII to FIII/FIV is more important than that from FIII to FIV for ovarian maturation. The number of upregulated genes was more than that of the downregulated genes, suggesting that increased gene expression was involved in the transition from FII to FIII/FIV. The representative pathways of DEGs were steroid biosynthesis, fatty acid biosynthesis, fatty acid elongation, glycerolipid metabolism, biosynthesis of unsaturated fatty acid, and α-linolenic acid metabolism. The differential expressions from the transcriptome sequencing were validated with real-time reverse-transcription polymerase chain reaction. We also found 13 genes in sexual development, female sex determination, gonadal development, ovarian maturation, ovarian follicle development, and oocyte development pathways, which were differently expressed among fish at FII, FIII, and FIV. We suggest that enhanced synthesis of steroid, unsaturated fatty acid, and α-linolenic acid may contribute to ovarian maturation of the Chinese sturgeon. These potential determinants provide a glimpse of the molecular architecture of ovary development in sturgeons.
Asunto(s)
Peces/metabolismo , Regulación del Desarrollo de la Expresión Génica/fisiología , Ovario/crecimiento & desarrollo , Ovario/metabolismo , Animales , Femenino , TranscriptomaRESUMEN
In tetrapods, kisspeptins are a group of peptides that play essential roles in the regulation of the Gonadotropin-releasing hormone secretion, and may participate in the feedback regulation of sex steroids as well. In this study, two kiss paralogs, designated as dskiss1 and dskiss2 were identified in Acipenser dabryanus. The full-length cDNA sequences of dskiss1 and dskiss2 are 1265 and 744 base pairs (bp), encoding 130 and 146 amino acids, respectively. Multiple sequence alignment indicated that both Kiss1 and Kiss2 decapeptides were highly conserved among vertebrates. Besides, Kiss1 of Dabry's sturgeon shared closer evolutionary relationship with the holostean species spotted gar (Lepisosteus oculatus), while Kiss2 of Acipenser dabryanus was conservatively grouped with the early sarcopterygian coelacanth (Latimeria chalumnae) in the phylogenetic analysis. Tissue distribution analysis showed that dskiss1 transcribed exclusively in the brain, whereas dskiss2 exhibited wider tissue distribution including brain, testis and ovary. Furthermore, male Dabry's sturgeons were intraperitoneally injected with 17ß-estradiol (E2) and the effect of E2 on hypothalamus kiss and its receptors kissr mRNA levels was evaluated by relative real-time PCR. The transcription levels of dskiss2 and dskissr1 were significantly increased by E2 injection (Pâ¯<â¯.05). However, the mRNA levels of dskiss1 and dskissr2 were not changed in E2-treated group compared to the control group. These results indicate that E2 exerts positive feedback effects through dskiss2/dskissr1 in male Dabry's sturgeon.
Asunto(s)
Estradiol/farmacología , Estrógenos/farmacología , Retroalimentación Fisiológica , Proteínas de Peces/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Kisspeptinas/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Proteínas de Peces/genética , Peces , Kisspeptinas/genética , Filogenia , Homología de Secuencia , Distribución TisularRESUMEN
Recent progress in germ cell transplantation techniques in fish has paved the way for the conservation of endangered species. Here, we developed an intraperitoneal germ cell transplantation procedure using Chinese and Dabry's sturgeon as donor and recipient species, respectively. Histological analysis revealed that primordial germ cells migrated on the peritoneal wall at 16 days post-hatch (dph) in Dabry's sturgeon. The genital ridges of Dabry's sturgeon (recipient) first formed at 28 dph, suggesting that for successful colonization of donor germ cells in the recipient gonads, the transplantation should be performed earlier than this age. Sexual dimorphism of gonadal structure was first observed at 78 dph. Gonadal germ cell proliferation was not seen in either sex during this period. Immunohistochemistry using the anti-Vasa antibody found that donor testes from 2-year-old Dabry's sturgeon mainly consisted of single- or paired-type A spermatogonia, while donor ovaries from 11.5-year-old Chinese sturgeon had perinucleolus stage oocytes and clusters of oogonia. Donor cells isolated from Dabry's sturgeon testes or Chinese sturgeon ovary labeled with PKH26 fluorescent dye were transplanted into the peritoneal cavity of the 7- or 8-dph Dabry's sturgeon larvae. More than 90% and 70% of transplanted larvae survived after 2 days post-transplantation (dpt) and 51 dpt, respectively. At 51 dpt, PKH26-labeled cells exhibiting germ cell-specific nuclear morphology and diameter were observed in excised recipient gonads by fluorescent and confocal microscopy. The colonization rate of allogeneic testicular germ cell transplantation (Group 1) was 70%, while that of two batches of xenogeneic ovarian germ cell transplantation (Group 2 and Group 3) were 6.7% and 40%, respectively. The ratio of colonized germ cells to endogenous germ cells was 11.96%, 5.35% and 3.56% for Group 1, Group 2 and Group 3, respectively. Thus, we established a germ cell transplantation technique for the critically endangered Chinese sturgeon using the most closely related species as a recipient and demonstrated the successful preparation of transplantable female germ cells from aged adult Chinese sturgeon.
Asunto(s)
Trasplante de Células/veterinaria , Conservación de los Recursos Naturales/métodos , Peces , Células Germinativas/trasplante , Animales , Cruzamiento , Trasplante de Células/métodos , Femenino , MasculinoRESUMEN
Germ cells are set aside from somatic cells early in embryogenesis, and are responsible for transmitting genetic information through generations. Vasa is a highly conserved germ cell marker across animal phyla, and widely used to label primordial germ cells. Dabry's sturgeon is a rare and endangered species distributed solely in the Yangtze River basin. Here, seven vasa isoforms, named Advasa1-7, were isolated and characterized in Dabry's sturgeon. RT-PCR and western blot analyses revealed that vasa mRNA and protein were mainly restricted to the testis and ovary, but exhibited sexually dimorphic expression. Cellular and subcellular localization uncovered that Advasa mRNA and protein displayed mitotic and meiotic expression in females, and mainly showed mitotic expression in males; surprisingly, they exhibited both cytoplasmic and nuclear expression in the ovarian germ cells, while showing exclusively cytoplasmic expression in the testicular germ cells. By microinjecting chimeric RNA consisting of the red fluorescent protein coding region and the Advasa 3'-untranslated region into embryos of Dabry's sturgeon, zebrafish and medaka, we demonstrated that it had the ability to visualize primordial germ cells (PGCs) in Dabry's sturgeon and zebrafish but not in medaka. It seemed that the machinery of vasa 3'UTR RNA localization was conserved between Dabry's sturgeon and ostariophysan, while possibly changed during the divergence of euteleosts and ostariophysan. Finally, Dabry's sturgeon PGCs moved on the yolk ball, and migrated toward the genital ridge via mesenchyme. Taken together, these results provide new information for vasa expression pattern and function, and lay a foundation for PGC cryopreservation and conservation of Dabry's sturgeon.
Asunto(s)
Regiones no Traducidas 3'/genética , ARN Helicasas DEAD-box/genética , Proteínas de Peces/genética , Peces/genética , Caracteres Sexuales , Secuencia de Aminoácidos , Animales , Western Blotting , Recuento de Células , Movimiento Celular , ARN Helicasas DEAD-box/química , ARN Helicasas DEAD-box/metabolismo , Embrión no Mamífero/citología , Embrión no Mamífero/metabolismo , Femenino , Proteínas de Peces/química , Proteínas de Peces/metabolismo , Peces/embriología , Técnica del Anticuerpo Fluorescente , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Células Germinativas/citología , Células Germinativas/metabolismo , Gónadas/citología , Gónadas/metabolismo , Masculino , Microinyecciones , Oryzias , Filogenia , Antígeno Nuclear de Célula en Proliferación/metabolismo , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Alineación de Secuencia , Pez CebraRESUMEN
The gene family DAZ (deleted in Azoospermia), including boule, dazl and DAZ, performs highly conserved functions in germ cell development and fertility across animal phyla. Differential expression patterns have been demonstrated for the family members in invertebrates and vertebrates including fish. Here, we report the identification of boule and dazl and their expression at both RNA and protein levels in developing and mature gonads of Chinese sturgeon (Acipenser sinensis). Firstly, the isolation of the boule and dazl genes in Chinese sturgeon and the observation of the two genes in coelacanth suggest that dazl originated after the divergence of bony fish from cartilaginous fish but before the emergence of the Actinistia. Quantitative real-time PCR and western blot analyses reveal that boule and dazl RNA and proteins are restricted to the testis and ovary. In situ hybridization and fluorescent immunohistochemistry show that the bisexual mitotic and meiotic germ cell expression of dazl RNA and protein is conserved in vertebrates, while Chinese sturgeon boule RNA and protein exhibit mitotic and meiotic expression in the testis, and also likely display mitotic and meiotic expression in female. Moreover, we directly demonstrate for the first time that sturgeon Balbiani body/mitochondrial cloud disperses in the cytoplasm of early developing oocytes and co-localizes with Dazl to some extent. Finally, urbilaterian boule may also have an ancestral function in oogenesis. Taken together, these results provide useful information on the evolution of DAZ family genes, expression patterns and functions in animal reproduction.
Asunto(s)
Proteínas de Peces/biosíntesis , Peces/metabolismo , Regulación de la Expresión Génica/fisiología , Ovario/metabolismo , Proteínas de Unión al ARN/biosíntesis , Testículo/metabolismo , Animales , Femenino , Masculino , Meiosis/fisiología , Mitosis/fisiología , Ovario/citología , Testículo/citologíaRESUMEN
The class V POU family genes, including pou5f1 and pou2, encode transcription factors critical for the maintenance of pluripotency in embryonic stem cells (ESC) and germ line cells in vertebrates. In the present study, the full-length cDNA of a pou2 ortholog in A. sinensis, Aspou2, was cloned and sequenced. This cDNA sequence is 2,853 base pairs in length and encodes a peptide of 431 amino acid residues. A comparison of the deduced amino acid sequence of Aspou2 with that of other vertebrate species showed that they were highly conserved in the POU domain, which shared 88 and 90% identity with that of zebrafish and medaka, respectively, and was 69, 67 and 67% identical to frog, mouse and human, respectively. RT-PCR analysis revealed that Aspou2 was detected in all tissues examined except for the liver, and high mRNA levels of Aspou2 were found in the muscle, pituitary and brain. During the embryogenesis and early larval development, the expression level of Aspou2 mRNAs decreased gradually apart from 1-day larvae that were not observed. Furthermore, Aspou2 seemed to raise with the development of gonads of immature Chinese sturgeons. These results suggested the possible involvement of Aspou2 in the nonpluripotent cells, pluripotent cells, embryogenesis, and gonad development.
Asunto(s)
Proteínas de Peces/metabolismo , Peces/fisiología , Factores de Transcripción/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Embrión no Mamífero/metabolismo , Desarrollo Embrionario , Femenino , Proteínas de Peces/genética , Peces/embriología , Expresión Génica , Larva/crecimiento & desarrollo , Larva/metabolismo , Masculino , Datos de Secuencia Molecular , Ovario/metabolismo , Filogenia , Estructura Secundaria de Proteína , ARN Mensajero/metabolismo , Factores de Transcripción/genéticaRESUMEN
Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289 bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1 day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.
Asunto(s)
Apolipoproteínas E/química , Apolipoproteínas E/genética , Peces , Secuencia de Aminoácidos , Animales , Clonación Molecular , Peces/genética , Perfilación de la Expresión Génica , Datos de Secuencia Molecular , Filogenia , Conformación Proteica , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Especificidad de la EspecieRESUMEN
The RNA helicase Vasa is a member of the DEAD box protein family that plays an indispensable role in germ cell determination in eukaryotes such as in Drosophila and Xenopus species. In this study, the grass carp homologue of the Drosophila vasa gene, Civasa (Ctenopharyngodon idella vasa) was obtained using degenerate primers in RT-PCR and 5' and 3' rapid amplification of cDNA end polymerase chain reaction from the grass carp ovary SMART cDNA. This cDNA sequence encodes a 670 amino acid residue protein that contains eight consensus regions for the DEAD box protein family, 9 arginine-glycine repeats and 9 arginine-glycine-glycine repeats, a common character of known Vasa homologues. CiVasa shows high identity to that of zebrafish and other animals, suggesting Vasa is highly conserved through evolution. RT-PCR analysis reveals that in grass carp tissues, both ovaries and testes contain large amounts of vasa gene transcripts whereas no Civasa transcript is detected in somatic tissues examined. The Civasa transcripts are present at a high level from the 2 cell stage to gastrula stages which indicated that Cicasa transcripts are maternally inherited. The predicted protein sequence, localization and conserved pattern of gene expression suggest that Civasa plays an important role in the germ cell determinant and development in grass carp as proposed for other teleost species.
Asunto(s)
Carpas/genética , ARN Helicasas DEAD-box/genética , Proteínas de Peces/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Carpas/embriología , Carpas/metabolismo , Clonación Molecular , ARN Helicasas DEAD-box/clasificación , ARN Helicasas DEAD-box/metabolismo , Embrión no Mamífero/metabolismo , Desarrollo Embrionario , Femenino , Proteínas de Peces/clasificación , Proteínas de Peces/metabolismo , Masculino , Datos de Secuencia Molecular , Ovario/metabolismo , Filogenia , ARN Mensajero/metabolismo , Alineación de Secuencia , Testículo/metabolismoRESUMEN
Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS(14) sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS(14) at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development.
Asunto(s)
Peces/genética , Somatostatina/biosíntesis , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Femenino , Expresión Génica , Masculino , Datos de Secuencia Molecular , Alineación de Secuencia , Somatostatina/genéticaRESUMEN
DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT1 gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1 antiserum to study the relationship of DMRT1 to sex reversal. One important finding is that the grouper DMRT1 is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1 genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad.
Asunto(s)
Organismos Hermafroditas , Procesos de Determinación del Sexo , Espermatogénesis , Testículo/metabolismo , Factores de Transcripción/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario , Femenino , Masculino , Datos de Secuencia Molecular , Ovario/crecimiento & desarrollo , Ovario/metabolismo , Perciformes , Filogenia , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Diferenciación Sexual , Testículo/crecimiento & desarrollo , Factores de Transcripción/genéticaRESUMEN
A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. In this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth.
Asunto(s)
Apolipoproteínas/genética , Regulación del Desarrollo de la Expresión Génica , Secuencia de Aminoácidos , Animales , Apolipoproteínas/metabolismo , Secuencia de Bases , Encéfalo/citología , Encéfalo/metabolismo , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Hígado/citología , Hígado/metabolismo , Masculino , Datos de Secuencia Molecular , Especificidad de Órganos/genética , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Tetraodontiformes/genéticaRESUMEN
A SMART cDNA plasmid library was constructed from protogyous greasy grouper (Epinephelus coioides) pituitary, and the full-length cDNAs of three gonadotropin (GTH) subunits common alpha, FSHbeta and LHbeta were cloned and sequenced from the library. The nucleotide sequences of common alpha, FSHbeta and LHbeta subunit cDNAs are 647, 594 and 574 bp in length, and encode for mature peptides of 94, 99 and 115 aa, respectively. High homology was observed by amino acid sequence alignment and identity comparison of the grouper mature peptides of common alpha, FSHbeta and LHbeta with that of other fishes. Phylogenetic tree analyses of the three GTH mature subunits revealed similar phylogeny relationships among the studied fish species. Three polyclonal antibodies were prepared from the in vitro expressed common alpha, FSHbeta and LHbeta mature proteins, respectively. Western blot analysis and immunofluoresence localization were performed on two typical stages of ovarian development stages in red-spotted grouper. Significant differences in protein expression levels of three gonadotropin subunits were revealed between the two ovarian development stages. In the individuals with resting ovary, common alpha was almost not detected in pituitaries, and FSHbeta and LHbeta expression levels were very low. While in the individuals with developing ovary, the expression of all three gonadotropin subunits reached to a high level. Immunofluoresence localization indicated that the grouper FSHbeta cells mainly distributed in the middle area of PPD, while the LHbeta cells distributed more widely, including in the area similar to the FSHbeta cells and at the external periphery of pituitary near to the PI side. The common alpha might be expressed in both FSHbeta and LHbeta cells. Double immunofluoresence localization further demonstrated FSHbeta and LHbeta expression in distinct cells in the PPD area, although the FSHbeta and LHbeta cells were detected in the identical area of PPD.
Asunto(s)
Gonadotropinas Hipofisarias/genética , Gonadotropinas Hipofisarias/metabolismo , Ovario/crecimiento & desarrollo , Perciformes/metabolismo , Hipófisis/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Ensayo de Inmunoadsorción Enzimática , Femenino , Hormona Folículo Estimulante de Subunidad beta/análisis , Hormona Folículo Estimulante de Subunidad beta/genética , Hormona Folículo Estimulante de Subunidad beta/metabolismo , Biblioteca de Genes , Hormonas Glicoproteicas de Subunidad alfa/análisis , Hormonas Glicoproteicas de Subunidad alfa/genética , Hormonas Glicoproteicas de Subunidad alfa/metabolismo , Gonadotropinas Hipofisarias/análisis , Hormona Luteinizante de Subunidad beta/análisis , Hormona Luteinizante de Subunidad beta/genética , Hormona Luteinizante de Subunidad beta/metabolismo , Datos de Secuencia Molecular , Ovario/citología , Perciformes/clasificación , Perciformes/genética , Filogenia , Hipófisis/química , Hipófisis/citología , Alineación de SecuenciaRESUMEN
We have cloned and characterized the full-length cDNA encoding thyroid-stimulating hormone beta-subunit (TSHbeta) from orange-spotted grouper Epinephelus coioides. It contains 913 nucleotides with an open reading frame encoding 146 amino acids with a 20 amino acid signal peptide. The grouper mature TSHbeta has 75, 70, 61, 59, 41, 42 and 40% identities to that of rainbow trout, Atlantic salmon, zebrafish, European eel, chicken, mouse and human, respectively. RT-PCR analysis indicated that the TSHbeta mRNA was expressed abundantly not only in pituitary but also in gonads. A more interesting finding is to reveal the differential TSHbeta expressions between the ovaries and the transitional gonads or testes in natural individuals of orange-spotted grouper and red-spotted grouper Epinephelus akaara, and in artificial sex reversal individuals of red-spotted grouper induced by MT feeding. In situ hybridization localization provided direct evidence that the TSHbeta was transcribed in the germ cells. In the growing oocytes, the TSHbeta transcripts were concentrated on the ooplasm periphery. In testicular tissues, the intensively expressed TSHbeta cells were found to be spermatogonia and spermatocytes in the spermatogenic cysts. This is the first report of a TSHbeta expressed in the gonads of any vertebrates in addition to the expected expression in the pituitary, and it expresses more transcripts in the gonads during sex reversal or testis than in the ovaries both in E. coioides and E. akaara. Importantly, the TSHbeta identification in germ cells allows us to further investigate the functional roles and the molecular mechanisms in gametogenesis of groupers, especially in sex reversal and in spermatogenesis.