RESUMEN

A novel and simple microfluidic device was developed for sorting 20 microm thick glass microbarcodes for imaging or scanning at the completion of a bead-based assay. Specifically, the microbarcodes are dried and kept from stacking on top of one another such that a monolayer of microbarcodes is created and the microbarcodes lay flat on a surface. The microbarcode sorting device consists of a reservoir, a sorting region, and a network of microchannels. With minimal microbarcodes loss, a monolayer of microbarcodes is created and trapped inside the sorting region for conveniently imaging or scanning. The device can also be used for any geometrical shaped beads with a range of thicknesses and can be adapted to a 96-well plate format for high throughput analysis.

Asunto(s)

Recolección de Datos/instrumentación , Procesamiento Automatizado de Datos/instrumentación , Microfluídica/instrumentación , Animales , Biotina/química , Bovinos , Procesamiento Automatizado de Datos/métodos , Fluoresceína-5-Isotiocianato/química , Humanos , Inmunoglobulina G/química , Microscopía Fluorescente , Microesferas , Propilaminas , Albúmina Sérica Bovina/química , Silanos/química , Estreptavidina/química