RESUMEN
The effects of vitamin C on the growth of human lung cultures and of vitamin C or L-cysteine on a human breast cancer culture (SK-Br-3) were assessed with and without exposure to fresh smoke from tobacco or marijuana cigarettes. When grown in the presence of vitamin C, lung cultures exposed or not exposed to either type of smoke showed a stimulation of growth and a significant decrease in mitotic abnormalities. However, abnormalities were much more marked in marijuana-exposed cultures than in tobacco-smoke-exposed ones. Nonexposed or tobacco-smoke-exposed breast cancer cultures, when grown in the presence of vitamin C, also showed acceleration of growth of epithelial cells, significant reduction in mitotic abnormalities, and occurrence of pseudoglandular structures, indicating differentiation. These alterations not only disappeared, but the cultures also became fibroblastic when they were returned to media without vitamin C. In contrast, vitamin C did not reduce mitotic abnormalities in marijuana-smoke-exposed breast cancer cultures, but stimulated abnormal growth and dedifferentiation. In nonexposed, tobacco-, or marijuana-smoke-exposed breast cancer cultures. L-cysteine evoked an acceleration of fibroblastic growth, which was not altered when the cultures were returned to media without L-cysteine.
Asunto(s)
Ácido Ascórbico/farmacología , Cannabis , Cisteína/farmacología , Pulmón/fisiología , Nicotiana , Plantas Tóxicas , Humo , Adulto , Neoplasias de la Mama/fisiopatología , Línea Celular , Células Cultivadas , ADN/análisis , Femenino , Feto , Humanos , Pulmón/efectos de los fármacos , Pulmón/patología , EmbarazoAsunto(s)
Ácido Ascórbico/farmacología , Cannabis , Transformación Celular Neoplásica , Nicotiana , Plantas Tóxicas , Humo , Neoplasias de la Mama/patología , División Celular/efectos de los fármacos , Transformación Celular Neoplásica/efectos de los fármacos , Células Cultivadas , Femenino , Humanos , Pulmón/citologíaAsunto(s)
Ácido Ascórbico/farmacología , Transformación Celular Neoplásica/efectos de los fármacos , Cisteína/farmacología , Pulmón/efectos de los fármacos , Humo , Animales , Cannabis , Cricetinae , Medios de Cultivo , ADN/metabolismo , Pulmón/patología , Lisosomas , Técnicas de Cultivo de Órganos , Plantas Tóxicas , NicotianaAsunto(s)
Ácido Ascórbico/farmacología , División Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Cisteína/farmacología , Cannabis , Células Cultivadas/citología , Medios de Cultivo , Humanos , Pulmón/citología , Plantas Tóxicas , Humo , NicotianaRESUMEN
After hamster lung cultures were exposed repeatedly to puffs of fresh smoke from 7 types of cigarettes containing variable amounts of particulate and gas vapour phase components, atypical growth and/or malignant cell transformation were observed within a period of 3-6 months. A positive correlation was demonstrable between high SH reactivity and high NO content of the gas vapour phase and malignant transformation. There was no positive correlation for the other analyzed components of the smoke, including tar content.
Asunto(s)
Neoplasias Pulmonares/etiología , Fumar/complicaciones , Compuestos de Sulfhidrilo/farmacología , Animales , Carcinógenos , Transformación Celular Neoplásica/efectos de los fármacos , Cricetinae , Técnicas de Cultivo , Pulmón/patología , Breas/análisisRESUMEN
High levels of gas vapour phase constituents in cigarette smoke enhanced abnormal growth and tumorigenicity in primary cultures of hamster lung cells regularly exposed over long periods to the smoke from one of three types of cigarette. The ultrastructure of such cells was investigated by electron microscopy. Cells from cultures exposed to low levels of gas vapour phase constituents resembled cells from control, non-exposed cultures, and were filled with secondary lysosomes of the autophagic vacuole type. These autophagic vacuoles contained dense particles similar in size to the nucleoids of certain viruses. Cells from cultures exposed to high levels of gas vapour phase constituents and cells from tumours formed in nude mice after subcutaneous injection of transformed cultures lacked autophagic vacuoles but contained a higher number of cytoplasmic microfilaments. R-type (equals H-type) virus particles were found in the ER cisternae of control and exposure cultures at all ages, whilst A-type virus particles were found in close association with mitochondria in a transformed, exposed culture and in control cells which also formed tumours in nude mice but only after a much longer culture period.