RESUMEN
A mixture of related metabolites (denoted OR-1) was isolated from the fermentation broth of Penicillium funiculosum together with mitorubrinic acid. Structurally OR-1 is glyceric acid esterified with C14-C18 fatty acids. Steady-state studies revealed that OR-1 behaved like an uncompetitive trypsin inhibitor with Ki 17.6 mumol/L.
Asunto(s)
Ácidos Glicéricos/metabolismo , Penicillium/metabolismo , Proteínas de Plantas/metabolismo , Benzoatos/metabolismo , Medios de Cultivo , Ésteres/metabolismo , Fermentación , Ácidos Glicéricos/química , Penicillium/crecimiento & desarrollo , Proteínas de Plantas/química , Inhibidores de Tripsina , alfa-Amilasas/antagonistas & inhibidoresRESUMEN
The optimum conditions for the production of (-)-mitorubrinic acid by mutant strain of Penicillium funiculosum CCM F-8080 were examined in shaken flask cultures. The highest production of this metabolite was achieved in a medium with glucose as the sole carbon source and (NH4)2HPO4 as the nitrogen source. The most suitable concentrations of the given sources for production of (-)-mitorubrinic acid are 80 g/l glucose and 2 g/l (NH4)2HPO4 (maximal production, 814.9 mg/l). We also observed the influence of concentration of L-phenylalanine and inorganic phosphate in culture medium on the production of (-)-mitorubrinic acid. The biological activity of mitorubrinic acid as trypsin inhibitor was determined (IC50 41.05 mumol/l).