RESUMEN

An assay system, based on the passive hemagglutination test and permitting the serodiagnosis of HIV infection with correct results in more than 99% of cases, has been developed. Three kinds of freeze-dried erythrocyte diagnostica (with shelf life exceeding 6 months), possessing high serological activity and sensitized with recombinant gene-engineering polypeptides, have been obtained. The proposed assay system is highly promising for mass examination of sera for the presence of antibodies to HIV due to the simplicity of assay techniques, the possibility of storing the diagnostica within a wide range of temperatures (4 degrees-30 degrees C) and obtaining results in a short time (3 hours).

Asunto(s)

Serodiagnóstico del SIDA/métodos , Pruebas de Hemaglutinación/métodos , Portador Sano/diagnóstico , Eritrocitos/inmunología , Reacciones Falso Positivas , Liofilización , Productos del Gen env , Productos del Gen gag , Infecciones por VIH/diagnóstico , Seropositividad para VIH/diagnóstico , VIH-1/inmunología , Humanos , Proteínas Recombinantes

Asunto(s)

Yersiniosis/diagnóstico , Infecciones por Yersinia pseudotuberculosis/diagnóstico , Eritrocitos/inmunología , Heces/microbiología , Técnica del Anticuerpo Fluorescente , Pruebas de Inhibición de Hemaglutinación , Pruebas de Hemaglutinación , Humanos , Factores de Tiempo , Infecciones por Yersinia pseudotuberculosis/microbiología