1.
Spectrochim Acta A Mol Biomol Spectrosc
; 185: 304-309, 2017 Oct 05.
Artículo
en Inglés
| MEDLINE
| ID: mdl-28599234
RESUMEN
Temperature-induced conformational transitions of poly-l-lysine were monitored with Fourier-transform infrared (FT-IR) spectroscopy between 10°C and 70°C. Chemometric analysis of dynamic IR spectra was performed by multivariate curve analysis-alternating least squares (MCR-ALS) of the amide I' and amide II' spectral region. With this approach, the pure spectral and concentration profiles of the conformational transition were obtained. Beside the initial α-helical, the intermediate random coil/extended helices and the final ß-sheet structure, an additional intermediate PLL conformation was identified and attributed to a transient ß-sheet structure.