RESUMEN

OBJECTIVE: The main aim of the present study was to detect bovine fetal DNA in the maternal circulation, a relatively unexplored subject in the literature. STUDY DESIGN: DNA was extracted from blood of 84 primipara cows (Bos indicus) at different gestational ages (30-270 days) and from 100 adult animals (50 males and 50 non-pregnant cows). The samples were analyzed using PCR with primers for TSPY gene. RESULTS: Molecular results matched the fetal phenotypic gender in all 47 male and 37 female fetuses, including early pregnancy, and in control animals. CONCLUSIONS: These results evidence a bovine transplacental fetal DNA passage.

Asunto(s)

Bovinos/genética , ADN/sangre , Feto/metabolismo , Intercambio Materno-Fetal/genética , Madres , Preñez , Animales , Bovinos/sangre , Bovinos/metabolismo , Femenino , Edad Gestacional , Masculino , Embarazo , Diagnóstico Prenatal/métodos , Diagnóstico Prenatal/veterinaria , Factores Sexuales