RESUMEN
This cross-sectional study was to assess the nutrients in terms of protein, fat, minerals, and hormones in heat-dried human placenta. Thirty heat-dried human placentas, 15 from male and 15 from female, were analyzed for protein (amino acids), fiber, fat, moisture, minerals (sodium, potassium, phosphorus, calcium, iron, magnesium, zinc, copper, manganese), hormones (estradiol, progesterone, testosterone, growth hormone). Heat-dried female human placentas had slightly higher fiber content than male, but protein and fat components were not different. Mineral levels in placentas were high especially sodium, potassium and phosphorus. There were no significant differences in the amount of minerals and hormonal profile between female and male placentas. However, hormone levels in heat-dried placenta were low compared to physiologic level in human beings. The results of this study suggest that the amount of nutrients particularly protein and minerals in heat-dried human placentas were enriched.
Asunto(s)
Hormonas/análisis , Valor Nutritivo , Placenta/química , Aminoácidos/análisis , Cromatografía Líquida de Alta Presión , Estudios Transversales , Grasas/análisis , Femenino , Calor , Humanos , Masculino , Minerales/análisis , Preservación de Órganos/métodos , Embarazo , Proteínas/análisis , Sensibilidad y Especificidad , Factores SexualesRESUMEN
The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37 degrees C in 5% CO2 for 24 h in the presence of each sealer, cells were stained with 0.4% SRB and the absorbance at 540 nm determined as measure of cell viability. For 51Cr-release assay, cells were labelled with 51Cr before testing with sealers, and radioactivity in the supernatant was measured in a liquid scintillation counter. Both techniques indicated that Apexit was the least toxic sealer. In view of the ease of conducting SRB staining for tests of cell viability, this may be the method of choice over 51Cr-release assay in the evaluation of endodontic sealer cytotoxicity.
Asunto(s)
Fibroblastos/efectos de los fármacos , Materiales de Obturación del Conducto Radicular/toxicidad , Análisis de Varianza , Animales , Radioisótopos de Cromo , Ratones , Rodaminas , Pruebas de ToxicidadRESUMEN
DNA topoisomerase II from Plasmodium falciparum was partially purified by FPLC using three columns: Econo-Pac Q, heparin-agarose and Mono Q. The enzyme showed ATP- and Mg2 +/- dependent activities in a decatenation assay, with optimum concentrations of 0.5 and 10 mM, respectively. Furthermore, highest activity was detected in the presence of 100 mM KCI. Enzyme decatenation activity was not inhibited by the DNA topoisomerase I inhibitor, camptothecin, but was sensitive to both prokaryotic and eukaryotic DNA topoisomerase II inhibitors.
Asunto(s)
ADN-Topoisomerasas de Tipo II/química , ADN-Topoisomerasas de Tipo II/aislamiento & purificación , ADN Protozoario/química , ADN Protozoario/aislamiento & purificación , Plasmodium falciparum/genética , Animales , Antiinfecciosos/farmacología , Camptotecina/farmacología , Cromatografía en Agarosa , ADN-Topoisomerasas de Tipo II/efectos de los fármacos , ADN Protozoario/antagonistas & inhibidores , ADN Protozoario/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Electroforesis en Gel Bidimensional , Células Eucariotas , Fluoroquinolonas , Pruebas de Sensibilidad Microbiana , Células Procariotas , Inhibidores de Topoisomerasa IIRESUMEN
The efficacy of magnesium sulfate was evaluated as the primary tocolytic agent in the management of patients at risk for premature delivery. Fifteen patients presenting with premature labor between 28 and 35 weeks gestation, given titrated dose intravenously magnesium sulfate till uterine contraction ceased, were prospectively studied. All patients received an additional oral beta-mimetic agent once labor was arrested till 36 weeks gestation. All cases had intact membranes and no contraindications for tocolytic therapy. Tocolysis for more than 72 hours was achieved in 14 cases (93.3%). Delayed tocolysis of more than 7 days was achieved in 13 cases (86.7%). The mean dosage to achieve tocolytic effect was 3.17 g/hr (SD 0.08). The mean serum magnesium level to achieve such effect was 5.38 mg/dl (SD 0.88). Magnesium sulfate was found to be easy to administer and clinically efficacious with minimal adverse effects. It may be used as the first line of tocolytic therapy where beta-sympathomimetic drugs are contraindicated.