RESUMEN

The development of simple and chemically defined liquid media for Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis and Crypto-occus neoformans according to their aminopeptidases profiles as amino acid requirement was described. When 1.5% purified agar was added, these media also supported excellent mycelial growth and sporulation of the deep mycoses. H. capsulatum was converted to and maintained in yeast phase when 0.1% L-cystine was added to the solid medium incubated at 37 degrees C.

Asunto(s)

Aminoácidos/metabolismo , Aminopeptidasas/metabolismo , Blastomyces/crecimiento & desarrollo , Cryptococcus neoformans/crecimiento & desarrollo , Cryptococcus/crecimiento & desarrollo , Hongos/crecimiento & desarrollo , Histoplasma/crecimiento & desarrollo , Paracoccidioides/crecimiento & desarrollo , Animales , Blastomyces/enzimología , Cryptococcus neoformans/enzimología , Medios de Cultivo , Cistina/metabolismo , Histoplasma/enzimología , Humanos , Ratones , Paracoccidioides/enzimología