RESUMEN
In Korea, genetically modified (GM) canola events derived from eleven single events have been authorized for food and feed, but not for cultivation. Therefore, the development of a rapid and accurate on-site detection method is crucial for the management of these approved GM canola events. In this study, ultrafast polymerase chain reaction (PCR) assays for the event-specific detection of eleven GM canola events were developed. The limit of detection (LOD) on DNA-based and powder-based GM canola samples of each primer set using the ultrafast PCR ranged from 0.1% to 0.01%, while the quantitative analysis of these ultrafast PCR assays, indicated that the correlation coefficient (R2) ranged from 0.98 to 0.9903. These results indicate that the developed assays may have sufficient specificity and LOD capacity to detect the eleven specific GM canola events for the attendant management and monitoring, thus preventing GM canola from contaminating the natural environment.
Asunto(s)
Brassica napus , Bioensayo , Brassica napus/genética , ADN de Plantas/genética , Límite de Detección , Plantas Modificadas Genéticamente/genética , Reacción en Cadena de la PolimerasaRESUMEN
As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events. To verify the specificity of event-specific primers, various GM crops of 3 GM soybean events, 6 GM maize events, 2 GM cotton events and 11 GM canola events were prepared. The limit of detection of the developed multiplex PCR was approximately 0.0125% for 3 GM canola events. Certified GM canola and stacked events were analyzed to validate the developed multiplex PCR. This study focuses on establishing multiplex PCR coupled with capillary electrophoresis for newly approved GM canola events and contributes to efficient monitoring GM canola samples in Korea.
RESUMEN
A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay's specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. The sensitivity of each assay was determined to range from 0.01% to 0.05% using DNA mixtures with different GM ratios, and it was validated by the results of three experimenters. The applicability of this study was tested by monitoring 23 processed foods containing soybeans. It was figured out that 13 of the 23 samples included GM soybeans. The prediction system combined with screening results will be helpful to trace the absence/presence of GM soybean events. This new qPCR array and prediction system for GM soybean detection provides rapid, convenient and reliable results to users.
RESUMEN
Highly porous carbon electrodes for supercapacitors with high energy storage performance were prepared by using a new precursor blend of aromatic polyimide (PI) and polyvinylidene fluoride (PVDF). Supercapacitor electrodes were prepared through the electrospinning and thermal treatment of the precursor blends of aromatic PI and PVDF. Microstructures of the carbonized PI/PVDF nanofibers were studied using Raman spectroscopy. Nitrogen adsorption/desorption measurements confirmed their high surface area and porosity, which is critical for supercapacitor performance. Energy storage performance was investigated and carbonized PI/PVDF showed a high specific capacitance of 283 F/g at 10 mV/s (37% higher than that of PI) and an energy density of 11.3 Wh/kg at 0.5 A/g (27% higher than that of PI) with high cycling stability.