Asunto(s)
Alcaligenes/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Diálisis Peritoneal/efectos adversos , Peritonitis/tratamiento farmacológico , Anciano , Ciprofloxacina/administración & dosificación , Femenino , Humanos , Imipenem/administración & dosificación , Fallo Renal Crónico/microbiología , Fallo Renal Crónico/terapia , Peritonitis/microbiologíaRESUMEN
PURPOSE: Ablation of thyroid remnants in patients with differentiated thyroid carcinoma and renal failure can be challenging because of the altered and variable clearance rates of iodine from the blood secondary to variations in dialysis protocols, which complicate the selection of the appropriate I-131 dose. The advent of recombinant human TSH allows a simpler approach to dosimetry and ablation without rendering the patient hypothyroid. Avoidance of hypothyroidism may be an important consideration for patients who are experiencing various morbidities from conditions associated with renal failure. METHOD: Three patients on dialysis, who had undergone total thyroidectomy and were euthyroid on L-thyroxine replacement, were given diagnostic doses of I-131 followed by blood and whole-body retention measurements through serial dialyses to determine individual blood clearance rates. After administration of rhTSH, each patient received an ablative dose of I-131 calculated to keep total body dose below 1 Gy. RESULTS: The treatments were administered without complications, and in follow-up imaging of 2 available patients, the ablations were demonstrated to be complete. CONCLUSION: Dosimetry performed on euthyroid dialysis patients permits I-131 dose selection and avoids the additional morbidity of hypothyroidism.
Asunto(s)
Radiofármacos/administración & dosificación , Radiofármacos/uso terapéutico , Insuficiencia Renal/complicaciones , Neoplasias de la Tiroides/radioterapia , Neoplasias de la Tiroides/cirugía , Tirotropina/administración & dosificación , Tirotropina/uso terapéutico , Adulto , Anciano , Protocolos Clínicos , Diabetes Mellitus Tipo 1/complicaciones , Femenino , Granulomatosis con Poliangitis/complicaciones , Terapia de Reemplazo de Hormonas , Humanos , Radioisótopos de Yodo/administración & dosificación , Radioisótopos de Yodo/efectos adversos , Radioisótopos de Yodo/uso terapéutico , Fallo Renal Crónico/complicaciones , Fallo Renal Crónico/terapia , Masculino , Persona de Mediana Edad , Enfermedad Pulmonar Obstructiva Crónica , Radiometría , Radiofármacos/efectos adversos , Proteínas Recombinantes/uso terapéutico , Diálisis Renal , Insuficiencia Renal/terapia , Estudios Retrospectivos , Tiroidectomía , Tirotropina/efectos adversos , Tiroxina/uso terapéuticoRESUMEN
The Arabidopsis genes CONSTANS-LIKE 1 (COL1) and CONSTANS-LIKE 2 (COL2) are predicted to encode zinc finger proteins with approximately 67% amino acid identity to the protein encoded by the flowering-time gene CONSTANS (CO). We show that the circadian clock regulates expression of COL1 and COL2 with a peak in transcript levels around dawn. We analyzed transgenic plants misexpressing COL1, COL2 and CO. Unlike CO, altered expression of COL1 and COL2 in transgenic plants had little effect on flowering time. However, analysis of circadian phenotypes in the transgenic plants showed that over-expression of COL1 can shorten the period of two distinct circadian rhythms. Experiments with the highest COL1 over-expressing line indicate that its circadian defects are fluence rate-dependent, suggesting an effect on a light input pathway(s).
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis/genética , Ritmo Circadiano , Proteínas de Unión al ADN/genética , Proteínas de Plantas/genética , Factores de Transcripción/genética , Dedos de Zinc , Arabidopsis/fisiología , Relojes Biológicos , Northern Blotting , Proteínas de Unión al ADN/metabolismo , Mediciones Luminiscentes , Fenotipo , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , ARN Mensajero/análisis , Factores de Transcripción/metabolismoRESUMEN
Several DNA-binding proteins with conserved basic region/leucine zipper domains (bZIP) have been isolated from parsley. They all recognise defined ACGT-containing elements (ACEs), including ACE(PcCHSII) in the Light Regulatory Unit LRU1 of the CHS promoter which confers light responsiveness. A new member of this Common Plant Regulatory Factor (CPRF) family, designated CPRF4a, has been cloned, which displays sequence similarity to HBP-1a from wheat, as well as to other plant bZIP proteins. CPRF4a specifically binds as a homodimer to ACE(PcCHSII) and forms heterodimers with CPRF1 but not with CPRF2. In adult parsley plants, CPRF2 and CPRF4a mRNAs are found in all tissues and organs in which the chalcone synthase gene CHS is expressed. In protoplasts from suspension cultured cells, UV irradiation (290-350 nm) did not cause an increase in levels of CPRF1, CPRF2, or CPRF4a mRNA, whereas the corresponding CPRF proteins accumulated within 15 min of light treatment. Furthermore, the rapid light-mediated increase of CPRF proteins was insensitive to transcriptional inhibitors, suggesting that a post-transcriptional mechanism controls CPRF accumulation. CPRFs as well as Arabidopsis thaliana G-box binding factors (GBFs) are selectively transported from the cytosol into the nucleus, as shown in an in vitro nuclear transport system prepared from evacuolated parsley protoplasts, indicating that cytosolic compounds are involved in regulated nuclear targeting of plant bZIP factors.
Asunto(s)
Proteínas de Unión al ADN/genética , Leucina Zippers/genética , Proteínas de Plantas/genética , ARN Mensajero/metabolismo , Factores de Transcripción/genética , Aciltransferasas/genética , Secuencia de Aminoácidos , Apiaceae , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico , Proteínas de Unión al ADN/metabolismo , Dimerización , Factores de Unión a la G-Box , Datos de Secuencia Molecular , Proteínas de Plantas/metabolismo , PlantasRESUMEN
Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase II. pKIWI110 also contains the reporter gene for ß-D-glucuronidase, and pLN26, the chalcone synthase antisense gene. Southern DNA analysis revealed that all the kanamycin-resistant transformants tested contained copies of the transgenes integrated in their genome. The two plants transformed with pKIWI110 show ß-D-glucuronidase expression in their mature leaves and selected transformants passed on the kanamycin-resistant phenotype to the F1 generation.
RESUMEN
Five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa cv. Hayward) were isolated from a library made from young fruit, 8-10 days after anthesis. One gene (pKIWI503) has low levels of expression in young fruit but is induced late in fruit development and during fruit ripening, and has some homology to plant metallothionein-like proteins. The other four genes are highly expressed in young fruit with reduced expression in the later stages of fruit development. pKIWI504 has strong homology to plant metallothionein-like proteins and pKIWI505 exhibits homology to the beta-subunit of the mitochondrial ATP synthase gene. The two other genes (pKIWI501 and 502) encode proteins with no significant homology to other known sequences.
Asunto(s)
Frutas/genética , Regulación Enzimológica de la Expresión Génica , Genes de Plantas/genética , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Clonación Molecular , Secuencia de Consenso , ADN Complementario/genética , Frutas/anatomía & histología , Frutas/enzimología , Frutas/crecimiento & desarrollo , Metalotioneína/biosíntesis , Metalotioneína/genética , Datos de Secuencia Molecular , Proteínas de Plantas/biosíntesis , ATPasas de Translocación de Protón/biosíntesis , ATPasas de Translocación de Protón/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Distribución TisularRESUMEN
Plantlets were regenerated from protoplasts of in vitro shoot cultures and leaf-derived de novo shoots of the chrysanthemum Dendranthema zawadskii x D. grandiflora. Isolated protoplasts reformed cell walls and then began to divide within 24 hours of culture in streaky plate agarose lenses surrounded by liquid V-KM medium. Twenty one days after isolation, 1 mm diameter callus clumps were transferred to shoot regeneration medium. After a further 33 days leaves became visible. Elongated shoots were rooted on half strength hormone-free MS medium.
Asunto(s)
Niño Hospitalizado , Vacunación , Niño , Preescolar , Análisis Costo-Beneficio , Humanos , Vacunación/economíaRESUMEN
A method has been developed to regenerate shoots directly from leaf pieces of the autumn flowering chrysanthemum Dendranthema indicum (L.) Des Moul (genotype Korean). Transgenic plants of this genotype were generated using transformation mediated by the disarmed strain of Agrobacterium tumefaciens LBA4404, containing either pKIWI110 or pGA643. Both pKIWI110 and pGA643 contain the selectable marker gene neomycin phosphotransferase II (NPTII) and pKIWI110 also contains the reporter gene ß-D-glucuronidase. Leaf pieces inoculated with pKIWI110 produced zones of blue cells two days after inoculation. Shoots from leaf pieces inoculated with pGA643 were selected on kanamycin. PCR and Southern analysis of shoots that were able to root on kanamycin confirmed the presence of the NPTII gene in the plant genome.
Asunto(s)
Puente Cardiopulmonar/efectos adversos , Enfermedad Coronaria/cirugía , Paro Cardíaco/terapia , Corazón Auxiliar , Adulto , Enfermedad Coronaria/fisiopatología , Femenino , Paro Cardíaco/etiología , Paro Cardíaco/fisiopatología , Humanos , Factores de Tiempo , Función Ventricular IzquierdaRESUMEN
In specimens sent for urgent total calcium measurement, failure to adjust for serum albumin concentration led to errors of diagnosis and treatment. Albumin adjustment is necessary, when measuring total serum calcium, for assessment of true calcium status.