RESUMEN
The sialylation of the lipooligosaccharide (LOS) of Neisseria meningitidis is mediated by the LOS sialyltransferase enzyme encoded by the lst gene. PCR using four sets of primers that targeted to different regions of the lst gene was used to survey the distribution of lst in different serogroups and LOS immunotypes of N. meningitidis as well as other Neisseria species. While the lst gene was found in N. meningitidis strains regardless of their capsular serogroup and LOS structures, the gene is also found in N. gonorrhoeae, N. lactamica, N. polysaccharea, and N. subflava biovar subflava. The presence of the lst gene in these organisms and the sialylation of their LOS antigens were discussed.
Asunto(s)
Proteínas Bacterianas , Lipopolisacáridos/inmunología , Neisseria meningitidis/metabolismo , Sialiltransferasas/análisis , Cartilla de ADN , ADN Bacteriano/análisis , Ensayo de Inmunoadsorción Enzimática , Lipopolisacáridos/metabolismo , Neisseria meningitidis/genética , Neisseria meningitidis/inmunología , Reacción en Cadena de la Polimerasa , SerotipificaciónRESUMEN
Six murine hybridoma monoclonal antibodies reactive with lipopolysaccharide antigens of Salmonella typhimurium were obtained from a fusion of immune spleen cells from mice immunized with S. typhimurium and NS1 myeloma cells. Four antibodies appeared to be specific for serogroup B salmonellae, while the remaining two antibodies were found to be cross-reactive with Salmonella paratyphi A. The exquisite specificities of the Salmonella serogroup B monoclonal antibodies were demonstrated by their unique reactivities with different serotypes of group B salmonellae but with neither other O serogroups of salmonellae nor a wide spectrum of standard strains of other bacterial species. Serotyping of salmonella strains by the slide agglutination method with two of the serogroup B-specific monoclonal antibodies demonstrated their usefulness as serotyping reagents for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory.
Asunto(s)
Anticuerpos Antibacterianos , Anticuerpos Monoclonales , Salmonella/inmunología , Pruebas de Aglutinación , Animales , Estudios de Evaluación como Asunto , Hibridomas/inmunología , Inmunoquímica , Indicadores y Reactivos , Ratones , Salmonella/clasificación , Serotipificación/métodosRESUMEN
We report a retrospective study of 198 trauma patients brought directly to a trauma operating room by an air ambulance system. Despite rapid transport, expert prehospital resuscitation, and the capability of early surgical intervention, the overall mortality was high (57%). There was no significant salvage of patients arriving without pulse, blood pressure or cardiac activity. Optimal trauma care failed to show encouraging results in this preselected group of patients with predominantly blunt and multisystem injury. The justification and cost effectiveness of this system of trauma care is discussed.
Asunto(s)
Aeronaves , Ambulancias , Quirófanos , Transporte de Pacientes , Heridas y Lesiones/cirugía , Adolescente , Adulto , Niño , Preescolar , Costos y Análisis de Costo , Servicios Médicos de Urgencia/economía , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Evaluación de Procesos y Resultados en Atención de Salud , Estudios Retrospectivos , Heridas y Lesiones/diagnóstico , Heridas y Lesiones/mortalidadRESUMEN
This is a report of a patient with a thrombosed Warren shunt and recurrent variceal hemorrhage who demonstrated compartmentalized venous hypertension in the mesenteric and gastrosplenic systems. Although the mesenteric presssure was normalized by the construction of a mesocaval interposition shunt, the gastrosplenic venous pressure remained elevated until splenectomy was performed. Warren shunt occlusion should be considered as a cause of segmental portal hypertension. Splenectomy may be a necessary adjunct in patients suitable for reshunting.