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PURPOSE: To use a combination of partial least squares regression and a machine learning approach to predict intraocular lens (IOL) tilt using preoperative biometry data. SETTING: Kepler University Clinic Linz, Linz, Austria. DESIGN: Prospective single-center study. METHODS: Optical coherence tomography, autorefraction, and subjective refraction were performed at baseline and 8 weeks after cataract surgery. In analysis I, only 1 eye per patient was included and a tilt prediction model was generated. In analysis II, a pairwise comparison between right and left eyes was performed. RESULTS: In analysis I, 50 eyes of 50 patients were analyzed. Difference in amount, orientation, and vector from preoperative to postoperative lens tilt was -0.13 degrees, 2.14 degrees, and 1.20 degrees, respectively. A high predictive power (variable importance for projection [VIP]) for postoperative tilt prediction was found for preoperative tilt (VIP = 2.2), pupil decentration (VIP = 1.5), lens thickness (VIP = 1.1), axial eye length (VIP = 0.9), and preoperative lens decentration (VIP = 0.8). These variables were applied to a machine learning algorithm resulting in an out of bag score of 0.92 degrees. In analysis II, 76 eyes of 38 patients were included. The difference of preoperative to postoperative IOL tilt of right and left eyes of the same individual was statistically relevant. CONCLUSIONS: Postoperative IOL tilt showed excellent predictability using preoperative biometry data and a combination of partial least squares regression and a machine learning algorithm. Preoperative lens tilt, pupil decentration, lens thickness, axial eye length, and preoperative lens decentration were found to be the most relevant parameters for this prediction model.
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Biometría , Implantación de Lentes Intraoculares , Lentes Intraoculares , Aprendizaje Automático , Facoemulsificación , Refracción Ocular , Tomografía de Coherencia Óptica , Humanos , Estudios Prospectivos , Biometría/métodos , Masculino , Refracción Ocular/fisiología , Femenino , Tomografía de Coherencia Óptica/métodos , Anciano , Persona de Mediana Edad , Migracion de Implante de Lente Artificial/fisiopatología , Seudofaquia/fisiopatología , Agudeza Visual/fisiologíaRESUMEN
Purpose: The purpose of this study was to investigate the diagnostic value of anterior segment optical coherence tomography (AS-OCT) scans for Pseudomonas keratitis. Methods: Patients with treatment-naïve keratitis underwent AS-OCT imaging. The following parameters were evaluated: corneal thickness (CT), infiltrate thickness (IT), infiltrate diameter (ID), tissue loss/gain, entropy, and distance of the lesion from the corneal pupillary center. Three different OCT devices were used for the analysis. The relationship between the detected pathogen and the OCT patterns was analyzed. Results: Nineteen eyes of 19 patients were included in the analysis: seven cases in the Pseudomonas group and 12 cases in the Gram-positive group. The mean (SD) values for the Pseudomonas and Gram-positive groups, respectively, were as follows: CT, 834 µm (165 µm) and 760 µm (120 µm); IT, 290 µm (152 µm) and 287 µm (84 µm); ID, 2067 µm (1470 µm) and 1307 µm (745 µm); distance to center, 3.0 mm (1.2 mm) and 3.0 mm (1.6 mm); epithelial defect, 1193 µm (586 µm) and 484 µm (615 µm); tissue gain, +31% (19%) and +10% (12%); and entropy level, 4.0 (0.8) and 3.9 (1.1). Conclusions: This study introduces novel insights by identifying specific OCT parameters that distinguish Pseudomonas keratitis, including a 30% tissue gain. These findings align with earlier research that underscores the potential of OCT in differentiating various pathogens causing keratitis. Translational Relevance: The findings of this study could be used to develop new diagnostic strategies for Pseudomonas keratitis. The OCT findings could be used to develop new biomarkers for the infection.
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Queratitis , Tomografía de Coherencia Óptica , Humanos , Queratitis/diagnóstico por imagen , Córnea/diagnóstico por imagen , Pseudomonas , PupilaRESUMEN
Purpose: To evaluate rotational stability, tilt and decentration of a new monofocal IOL with a 7.0 mm optic and frame haptics.Methods: Prospective post-market clinical follow-up study at the Kepler University Hospital Linz, Austria. An Aspira-aXA (HumanOptics, Germany) was implanted in 74 eyes of 42 cataract patients. The lens was manufactured with toric markings. IOL rotational stability was evaluated by comparing its position at the end of surgery (EoS) versus 1 day, 1 week, 1 month and 4 months postoperatively. IOL tilt and decentration were measured using a Scheimpflug camera at 1 week, 1 month and 4 months.Results: Median absolute IOL rotation was 1.42 degrees (n = 52; mean = 2.18 ± 2.23°) within 1 day after surgery and was significantly higher compared to all later intervals (median <1.0 degree; P = .001). At the 4 months follow-up, IOL rotation was within 5.0 degrees in 85% of the eyes (n = 40) and within 10.0 degrees in 98% (n = 46) of the eyes. The only eye with an IOL rotation of ≥ 10.ty0 degrees (EoS vs. 1 day) had an AL of 26.45 mm. At the last follow-up, the IOL vertical and horizontal tilt referenced to the pupillary axis was in average less than 1.5 degrees in both eyes (n = 54; maximum 5.85°). Decentration in both meridians was on average less than 0.10 mm in both eyes (maximum 0.30 mm).Conclusion: The one-piece Aspira-aXA IOL showed good and stable positioning within the capsular bag over a 4 months period.
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Migracion de Implante de Lente Artificial/fisiopatología , Implantación de Lentes Intraoculares , Lentes Intraoculares , Facoemulsificación , Seudofaquia/fisiopatología , Rotación , Anciano , Anciano de 80 o más Años , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Óptica y Fotónica , Fotograbar/instrumentación , Vigilancia de Productos Comercializados , Estudios Prospectivos , Diseño de Prótesis , Refracción Ocular/fisiologíaRESUMEN
BACKGROUND: Pupil examination represents a diagnostic and prognostic test in the management of several neurological diseases. Infrared video pupillometry (IVP) is the gold standard, since it is not routinely available, a noninvasive bedside ultrasound assessment has been proposed as an alternative. The aim of this study was to assess the feasibility and reproducibility of ultrasound pupillometry (UP) in comparison with IVP. MATERIALS AND METHODS: 81 subjects (43 men and 38 women, mean age: 52â±â20 years and 49â±â19 years, respectively) with no history of neurophthalmologic disease were enrolled. UP was performed with a 12-MHz linear probe according to current guidelines for orbital insonation. Light and painful stimuli were applied to test pupillary light reflex (PLR) and ciliospinal reflex (CR). In 30 of these subjects IVP examination was performed additionally to obtain intra-observer and inter-observer agreement. RESULTS: Increasing age was associated with a decreased pupillary diameter (PD) at rest, after PLR and CR (R -0.728, pâ<â0.01, R -0.643, pâ<â0.01, R 0.674, pâ<â0.001 respectively), while no association was noticed with time to constriction/dilation. UP measurements were reproducible (rate of inter- and intra-observer agreement: R 0.979, pâ<â0.01, R 0.946, pâ<â0.01 respectively) and concordant with IVP (PLR R 0.831, pâ<â0.01; CR R 0.879, pâ<â0.01). CONCLUSION: According to our study, ultrasound pupillometry is a feasible and reliable technique for bedside pupillary function assessment, and is a good alternative to infrared video pupillometry. Moreover, it represents the only way for functional pupillary assessment in patients with periorbital hematoma.
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Pupila , Reflejo Pupilar , Ultrasonografía , Adulto , Anciano , Femenino , Humanos , Luz , Masculino , Persona de Mediana Edad , Reproducibilidad de los ResultadosRESUMEN
PURPOSE: To compare the lens tilt after apex-centered capsulotomy with standard pupil-centered capsulotomy. SETTING: Clinical practice. DESIGN: Prospective, randomized, investigator-masked trial. METHODS: This prospective, randomized, investigator masked study included 40 patients with age-related cataract scheduled for femtosecond laser-assisted cataract surgery (FLACS) in both eyes. The main outcome was the tilt of the intraocular lens (IOL), measured with the IOLMaster 700. RESULTS: The study enrolled 80 eyes of 40 patients. The mean distance from the center of the apex-centered capsulotomy to the pupil-centered capsulotomy was 175.8 ± 97.2 µm. The amount of IOL tilt was not significantly reduced with the new technique (P > .05). Tilt orientation was more stable in eyes treated with apex-centered capsulotomy. CONCLUSIONS: Centration of the capsulotomy on the lens apex in FLACS does not influence the amount of IOL tilt but the orientation stability. The clinical importance of this finding has to be further elucidated.
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Extracción de Catarata , Catarata , Terapia por Láser , Capsulorrexis , Catarata/complicaciones , Humanos , Rayos Láser , Implantación de Lentes Intraoculares , Estudios Prospectivos , PupilaRESUMEN
PURPOSE: To study the intraocular pressure (IOP) variation during vacuum and docking of femtosecond laser application. SETTING: Department for Ophthalmology, Kepler University Hospital, Johannes Kepler University Linz, Austria. DESIGN: Experimental study. METHODS: Intracameral intraocular pressure (IOP) was monitored in 30 porcine ex vivo models using a dual femtosecond laser platform (VICTUS). Five different baseline IOP levels (10 to 30 mm Hg) were assessed during fluid-filled (liquid) docking, full-contact (applanating) docking, and vacuum application without docking. RESULTS: Raising the baseline intracameral IOP from 10 to 30 mm Hg increased the IOP from 27.55 mm Hg ± 5.93 (SD) to 56.85 ± 6.10 mm Hg in the fluid-filled docking group, and from 60.91 ± 8.41 mm Hg to 90.11 ± 6.64 mm Hg in the full-contact docking group. The full-contact docking procedure per se (excluding vacuum effect) increased the IOP by 61.7% (+36.58 ± 5.84 mm Hg). In contrast, fluid-filled docking (excluding vacuum effect) raised the IOP by only 3.1% (+0.72 ± 2.71 mm Hg). Each 5 mm Hg reduction of baseline IOP resulted in a mean IOP reduction of 7.33 ± 3.05 mm Hg in the fluid-filled group, and 7.30 ± 6.88 mm Hg in the full-contact docking group (P < .001). Decreasing baseline intracameral IOP from 15 to 10 mm Hg resulted in the highest IOP reduction between all subgroups (P < .01). CONCLUSIONS: Baseline IOP, docking procedures, and vacuum influenced IOP during femtosecond laser application in a synergistic manner. Lowering the baseline intracameral IOP resulted in a significantly lower IOP during fluid-filled docking for cataract surgery and full-contact docking for refractive surgery. Full-contact (applanating) docking resulted in a significantly higher IOP elevation compared with similar IOP values found during fluid-filled (liquid) docking and the application of the suction ring alone without docking.
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Extracción de Catarata/métodos , Catarata/terapia , Presión Intraocular/fisiología , Terapia por Láser/métodos , Procedimientos Quirúrgicos Refractivos/métodos , Animales , Catarata/fisiopatología , Modelos Animales de Enfermedad , Porcinos , VacioRESUMEN
PURPOSE: To evaluate the effect of preoperative intraocular pressure (IOP) and the vacuum level on IOP during femtosecond laser-assisted cataract surgery. METHODS: Intraocular pressure was measured in 40 enucleated porcine eyes by intracameral manometry prior, during and after vacuum application using the VICTUS femtosecond laser platform (Bausch&Lomb, Technolas Perfect Vision GmbH, Germany). Twenty combinations of different preoperative IOP levels (12, 16, 20 and 24 mmHg) and different vacuum levels (350-550 mbar) were investigated. RESULTS: Multivariate regression analysis indicated that both the vacuum level (beta = 0.138; p < 0.001) but much stronger the preoperative IOP (beta = 0.861; p < 0.001) were predictive factors for IOP rise during vacuum application. Mean IOP was 28.23 ± 3.86, 34.23 ± 3.92, 40.35 ± 4.41 and 46.82 ± 4.11 mmHg in groups with baseline IOP of 12, 16, 20 and 24 mmHg, respectively. In the 350, 450 and 550 mbar group, and mean IOP was 35.85 ± 7.85, 37.33 ± 7.90 and 39.00 ± 8.04 mmHg, respectively. Lowering the preoperative IOP by 2 mmHg and reducing the vacuum from maximum to minimum resulted in a similar reduction in IOP during vacuum application (-3.10 ± 0.79 mmHg versus -3.15 ± 0.88 mmHg; p = 0.015). Furthermore, decreasing the baseline IOP from 20 to 12 mmHg resulted in a 30.0% reduction in intraoperative IOP. CONCLUSION: Preoperative IOP was a stronger predictive factor for intraoperative IOP rise than the applied vacuum level. Measurements and critical interpretation of preoperative IOP in a preliminary examination could help estimating the individual risk of significant IOP rise during femtosecond laser-assisted cataract surgery and could help taking early countermeasures in selected cases. Due to the porcine ex vivo model, further studies are needed to verify these findings.
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Extracción de Catarata/métodos , Presión Intraocular/fisiología , Complicaciones Intraoperatorias/diagnóstico , Terapia por Láser/efectos adversos , Hipertensión Ocular/diagnóstico , Animales , Modelos Animales de Enfermedad , Complicaciones Intraoperatorias/etiología , Complicaciones Intraoperatorias/fisiopatología , Hipertensión Ocular/etiología , Hipertensión Ocular/fisiopatología , Valor Predictivo de las Pruebas , Periodo Preoperatorio , Factores de Riesgo , Porcinos , Tonometría Ocular , VacioRESUMEN
PURPOSE: To investigate the impact of spectral domain optical coherence tomography (SD-OCT) morphological predictive markers on visual acuity and outcome using ocriplasmin for macular hole and vitreomacular traction syndrome. METHODS: A series of 40 patients in a retrospective study received intravitreal ocriplasmin. The primary endpoint was defined as morphological resolution of vitreomacular traction or closure of a macular hole. We analyzed the impact of pre- and postinjection SD-OCT findings on the outcome and visual acuity. RESULTS: Thirteen of the 40 patients benefited from treatment. Statistical correlation between baseline characteristics and outcome revealed that higher foveal thickness (p = 0.018) and nontractional epiretinal membranes (p = 0.05) resulted in a worse outcome. In treatment success best corrected visual acuity gained was 9 (SD 12) letters and in failure 1 (SD 9) letter. We could not observe an influence of preinjection SD-OCT findings and other factors on visual outcome. CONCLUSION: We could confirm the therapeutic effect of ocriplasmin injections. SD-OCT morphological factors that influence treatment success and visual acuity were determined.
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Fibrinolisina/administración & dosificación , Mácula Lútea/patología , Fragmentos de Péptidos/administración & dosificación , Perforaciones de la Retina/tratamiento farmacológico , Tomografía de Coherencia Óptica/métodos , Cuerpo Vítreo/patología , Desprendimiento del Vítreo/tratamiento farmacológico , Anciano , Femenino , Estudios de Seguimiento , Humanos , Inyecciones Intravítreas , Estudios Longitudinales , Mácula Lútea/efectos de los fármacos , Masculino , Perforaciones de la Retina/diagnóstico , Estudios Retrospectivos , Resultado del Tratamiento , Agudeza Visual , Cuerpo Vítreo/efectos de los fármacos , Desprendimiento del Vítreo/diagnósticoRESUMEN
Antiphospholipid syndrome (APS) is an autoimmune disorder associated with arterial/venous thrombosis. Cardiac manifestations of APS include valve stenosis/insufficiency, coronary artery disease and myocardial dysfunction presenting as dilated cardiomyopathy. In the following report, we present the case of a man with primary APS, hypertrophic non-obstructive cardiomyopathy and hypotelorism-a combination that has not yet been reported in the literature.
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Síndrome Antifosfolípido/complicaciones , Cardiomiopatía Hipertrófica/diagnóstico por imagen , Adulto , Cardiomiopatía Hipertrófica/etiología , Angiografía por Tomografía Computarizada , Diagnóstico Diferencial , Facies , Humanos , MasculinoRESUMEN
PURPOSE: To evaluate the effects of current hygiene standards for the enucleation of postmortem eyes by investigating the number of microorganisms during subsequent steps of decontamination and tissue processing. MATERIALS AND METHODS: This prospective, non-randomized cohort study includes 184 postmortem eyes of 92 human donors. Enucleation was performed, according to an ophthalmic surgical procedure. Two groups were generated as follows: right eyes were allocated to group A, left eyes were allocated to group B. In group A, a mucosal disinfectant was used accessorily. Conjunctival smears were taken to examine germ load in both groups before any intervention, in group A after mucosal disinfection, in both groups after transportation of the whole globes in transport fluid, and in both groups after a bath in 0.75% povidone iodine solution for at least 3 minutes just before preparation of the corneoscleral disc. Smears were sent to the local microbiologic laboratory in an aseptic package for testing. RESULTS: All smears showed multiple contaminations (n = 184/184 eyes) before treatment with povidone iodine solution. Contamination was in both groups significantly prevented using the treatment strategy of an at least 3-minute bath in 0.75% povidone iodine solution (n = 1/184 eyes; p < 0.01) As a side effect, almost every eye of group A and none of group B showed brown iodine staining corresponding to corneal epithelial erosion. CONCLUSIONS: An aseptic setting for donor enucleation similar to a surgical procedure seems not to influence the outcome of germ colonization. The most effective step to decontaminate donor eyes is to use 0.75% povidone iodine solution for at least 3 minutes.
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Córnea/efectos de los fármacos , Trasplante de Córnea , Descontaminación/métodos , Bancos de Ojos , Povidona Yodada/farmacología , Donantes de Tejidos , Recolección de Tejidos y Órganos/métodos , Antiinfecciosos Locales/farmacología , Infecciones del Ojo/prevención & control , Estudios de Seguimiento , Humanos , Estudios ProspectivosRESUMEN
PURPOSE: The aim of this study was to evaluate the safety and efficacy of deep sclerokeratodissection (DSKD), a new nonpenetrating technique in glaucoma surgery. MATERIALS AND METHODS: Retrospective comparison between patients treated with DSKS or deep sclerectomy (DS) between 2013 and 2014. In DSKD, the first and only flap is dissected directly into clear cornea with unroofing Schlemm's canal. Beside routine clinical follow-up (visual acuity, intraocular pressure [IOP] readings, slit lamp and fundus examination), postoperative ultrasound biomicroscopy (UBM) investigation and quality of life (QoL) assessment were performed. Statistically significant differences were determined by parametric or nonparametric tests, depending on normality. RESULTS: Twelve (38.7%) DSKDs and 19 (61.3%) conventional DS' were included in this analysis. IOP decreased significantly from 21.5 ± 9.2 mmHg to 6.2 ± 5.4 mmHg on day 1, 13.4 ± 7.7 at 1 month, 12.0 ± 4.1 at 3 months, 12.5 ± 3.1 mmHg at 6 months, and 13.4 ± 4.3 mmHg at 12 months (P < 0.01). No significant difference in the IOP was observed between the two groups at any follow-up (P > 0.1). There was no significant difference in intra- and post-operative complications, the morphology of the surgical site in the UBM as well as in the QoL assessment. CONCLUSION: The results indicate that DSKD is a safe and efficient new variant of nonpenetrating glaucoma surgery. IOP can be lowered as effectively compared to conventional DS, with a similarly low rate of complications. Further reports are necessary to confirm these results.
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Cirugía Filtrante/métodos , Glaucoma de Ángulo Abierto/cirugía , Presión Intraocular , Esclerótica/cirugía , Colgajos Quirúrgicos , Anciano , Femenino , Estudios de Seguimiento , Glaucoma de Ángulo Abierto/diagnóstico , Glaucoma de Ángulo Abierto/fisiopatología , Humanos , Masculino , Microscopía Acústica , Estudios Retrospectivos , Esclerótica/diagnóstico por imagen , Resultado del Tratamiento , Agudeza VisualRESUMEN
PURPOSE: To determine whether erufosine alone or erufosine-loaded intraocular lenses (IOLs) can inhibit growth of human lens epithelial cells after a single administration in the human capsular bag model. SETTING: Laboratory for Cell Biology, Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. DESIGN: Experimental study. METHODS: Sixteen human cadaver eyes had sham cataract surgery. The capsular bag was transferred into cell culture. The tissue was exposed to the half maximum inhibitory concentrations of erufosine alone for 72 hours; solvent-only tissue served as a control. Erufosine is a potent inhibitor of phosphoinositide-3-kinase, a downstream kinase with major implications in posterior capsule opacification (PCO) pathogenesis. The IOLs were soaked with erufosine and implanted in the capsular bags; unsoaked IOLs served as controls. For both settings, the time until confluence of the capsular bag was measured. Cell growth was observed and photodocumented. RESULTS: Erufosine as a single therapeutic agent increased the time until confluence of the capsular bag, but not significantly compared with the control. When IOLs were soaked with erufosine, a long-term prophylactic effect was observed in this organ model for PCO, which is known to closely reflect the clinical situation. CONCLUSION: Erufosine-soaked IOLs effectively inhibited PCO formation as seen in long-term organ culture and might become of clinical relevance. FINANCIAL DISCLOSURES: Drs. Kampik and Eibl-Lindner are inventors of IOLs treated with alkylphosphocholines for pharmacological after-cataract prophylaxis, patent international application PCT/EP2010/051490. No other author has a financial or proprietary interest in any material or method mentioned.
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Opacificación Capsular/prevención & control , Portadores de Fármacos , Inhibidores Enzimáticos/farmacología , Lentes Intraoculares , Organofosfatos/farmacología , Inhibidores de las Quinasa Fosfoinosítidos-3 , Cápsula Posterior del Cristalino/efectos de los fármacos , Compuestos de Amonio Cuaternario/farmacología , Adulto , Anciano , Humanos , Cápsula del Cristalino , Implantación de Lentes Intraoculares , Persona de Mediana Edad , Modelos Biológicos , Técnicas de Cultivo de Órganos , Donantes de TejidosRESUMEN
PURPOSE: Age-related macular degeneration (AMD) is one of the leading causes of blindness. Degeneration of the retinal pigment epithelium (RPE) is pathognomonic for the disease, and oxidative stress plays an important role in the pathogenesis of this disease. This study investigates potential antiapoptotic and cytoprotective effects of idebenone on cultured RPE cells (ARPE-19) under conditions of oxidative stress. METHODS: ARPE-19 cells were treated with 1-100 µ
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Antioxidantes/farmacología , Senescencia Celular/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Epitelio Pigmentado de la Retina/citología , Ubiquinona/análogos & derivados , Proteína X Asociada a bcl-2/metabolismo , Western Blotting , Muerte Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Humanos , Peróxido de Hidrógeno/toxicidad , Oxidantes/toxicidad , Proteínas Proto-Oncogénicas c-bcl-2/genética , ARN Mensajero/genética , Especies Reactivas de Oxígeno/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Epitelio Pigmentado de la Retina/metabolismo , Ubiquinona/farmacología , Proteína X Asociada a bcl-2/genéticaRESUMEN
BACKGROUND: The aim of this work is to investigate the biocompatibility and staining properties of DSS: 3,3'-Di-(4-sulfobutyl)-1,1,1',1'-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS). METHODS: Dye concentrations of 0.5, 0.25, and 0.1% were evaluated (290 and 295 mOsm). Toxicity was assessed using a colorimetric test measuring the inhibition of ARPE 19 cell, human primary RPE cell, and human Müller cell proliferation. Exposure time was 30, 60, 120, and 300 s. Indocyanine green (ICG) (0.5, 0.25, and 0.1%) served as a control. Cells were also illuminated with plain white light (750 mW/cm(2)) for 10 min to assess phototoxic effects. Besides staining of porcine and human lens capsule, internal limiting membrane (ILM)-staining was assessed by applying 0.25 and 0.5% DSS over the macula in two human post-mortem eyes. RESULTS: DSS of 0.25 and 0.1% showed no toxic effect on primary RPE cells and MIO-M1cells, and 0.5, 0.25, and 0.1% for ARPE-19 cells. In MIO-M1cells, 0.5% dye showed a significant reduction of mitochondrial dehydrogenase activity only following an exposure time of 300 s. Following illumination, ICG showed a significantly more pronounced effect on cell viability in primary RPE cells and MIO-M1cells compared to DSS. The absorption maximum is found at 591 nm; the even more bathochromic fluorescence proceeds with a common Stokes' shift where maxima at 620 and 660 nm with a quantum yield of 32% were found. The fluorescence is sufficiently hypsochromic and the fluorescence quantum yield high enough for an easy visual detection. The contrast and staining properties at the ILM were excellent and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted. CONCLUSIONS: Our results indicate that this new cyanine dye DSS may represent an alternative for ILM staining due to its matched absorption concerning visibility and fluorescence qualities as well as its good biocompatibility.
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Membrana Basal/efectos de los fármacos , Materiales Biocompatibles , Carbocianinas/síntesis química , Carbocianinas/toxicidad , Colorantes/síntesis química , Colorantes/toxicidad , Anciano , Animales , Membrana Basal/patología , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Humanos , Verde de Indocianina/toxicidad , Cápsula del Cristalino/efectos de los fármacos , Cápsula del Cristalino/patología , Luz , Ensayo de Materiales , Neuronas Retinianas/efectos de los fármacos , Neuronas Retinianas/patología , Neuronas Retinianas/efectos de la radiación , Epitelio Pigmentado de la Retina/efectos de los fármacos , Epitelio Pigmentado de la Retina/patología , Epitelio Pigmentado de la Retina/efectos de la radiación , Coloración y Etiquetado/métodos , PorcinosRESUMEN
Cyanine dyes were prepared as optical contrast media for supporting the surgery of the lamina limitans interna (LLI) of the retina and other structures of the human eye. Their absorption spectra were adapted both to the spectral sensitivity of the human eye and to standard illumination. The contrast could be further amplified by the application of the strong fluorescence of the dyes used. The binding of the dyes to various surfaces was studied. No toxic effects could be detected for the applied dyes.
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Carbocianinas/síntesis química , Carbocianinas/metabolismo , Medios de Contraste , Colorantes Fluorescentes , Procedimientos Quirúrgicos Oftalmológicos , Retina/cirugía , Animales , Carbocianinas/química , Carbocianinas/toxicidad , Medios de Contraste/síntesis química , Medios de Contraste/química , Medios de Contraste/metabolismo , Medios de Contraste/toxicidad , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/química , Colorantes Fluorescentes/metabolismo , Colorantes Fluorescentes/toxicidad , Humanos , Estructura Molecular , Unión Proteica , PorcinosRESUMEN
OBJECTIVE: The present study assessed the expression of p16 and epidermal growth factor receptor (EGFR) in patients with adenocarcinoma of the uterine cervix to determine their influence on prognosis and to evaluate a possible association between their expression and various clinicopathologic parameters. METHODS: p16 and EGFR expression was investigated by immunohistochemistry from paraffin-embedded tissue in 39 patients with adenocarcinoma of the uterine cervix. The immunohistochemical findings were correlated with different clinicopathologic parameters of the patients. RESULTS: p16 was expressed in 56% of the patients. A trend towards increased lymph vascular space invasion was observed in p16 positive tumors (p = 0.06). There was no statistically significant association between p16 expression and clinical stage, age, histology, tumor size, tumor grade, lymph node status and recurrence disease (p > 0.05). p16 expression did influence neither disease-free nor overall survival (p > 0.05). EGFR was expressed in 44% of the patients. There was no statistically significant correlation between EGFR expression and clinical stage, age, histology, tumor size, tumor grade, lymph vascular space invasion, lymph node status and recurrence disease (p > 0.05). EGFR expression did influence neither disease-free nor overall survival (p > 0.05). CONCLUSION: p16 and EGFR are frequently expressed in adenocarcinoma of the uterine cervix. Our study observed a trend towards increased lymph vascular space invasion in p16 positive tumors. Otherwise, the expression of the investigated parameters did not correlate with any clinicopathologic parameters and had no influence on overall and disease-free survival. So far, the investigation of p16 and EGFR is of limited use to assess patients' prognosis and guide clinical management.
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Adenocarcinoma/metabolismo , Biomarcadores de Tumor/biosíntesis , Receptores ErbB/biosíntesis , Proteínas de Neoplasias/biosíntesis , Neoplasias del Cuello Uterino/metabolismo , Adenocarcinoma/mortalidad , Adenocarcinoma/patología , Adulto , Cuello del Útero/metabolismo , Inhibidor p16 de la Quinasa Dependiente de Ciclina , Supervivencia sin Enfermedad , Femenino , Humanos , Inmunohistoquímica , Persona de Mediana Edad , Estadificación de Neoplasias , Pronóstico , Estudios Retrospectivos , Neoplasias del Cuello Uterino/mortalidad , Neoplasias del Cuello Uterino/patología , Adulto JovenRESUMEN
OBJECTIVE: The expression of oestrogen and progesterone receptors in patients with adenocarcinoma of the uterine cervix was examined in order to determine their influence on prognosis and to evaluate the association between the steroid receptor expression and various clinicopathologic parameters. PATIENTS AND METHODS: Oestrogen and progesterone receptor expression was investigated by immunohistochemistry from paraffin-embedded tissue in 39 patients with adenocarcinoma of the uterine cervix. The immunohistochemical findings were correlated with various clinicopathological parameters of the patients. RESULTS: Oestrogen and progesterone receptors were expressed in 39% and 33% of the patients, respectively. The relationship between oestrogen and progesterone receptor expression and clinical stage, age, histology, tumour size, grade, lymph-vascular space invasion and lymph node status did not reach statistical significance (p>0.05). Neither oestrogen nor progesterone receptor expression significantly influenced disease-free and overall survival (p>0.05). CONCLUSION: Oestrogen and progesterone receptors were frequently expressed in adenocarcinoma of the uterine cervix. However, their expression did not correlate with clinicopathological parameters and had no influence on overall and disease-free survival. Thus, the investigation of steroid receptors adds little additional information to the clinical management and fails to play a prognostic role in cervical adenocarcinoma.