RESUMEN
Agrobacterium tumefaciens is a plant pathogen capable of transferring a defined segment of DNA to a host plant, generating a gall tumor. Replacing the transferred tumor-inducing genes with exogenous DNA allows the introduction of any desired gene into the plant. Thus, A. tumefaciens has been critical for the development of modern plant genetics and agricultural biotechnology. Here we describe the genome of A. tumefaciens strain C58, which has an unusual structure consisting of one circular and one linear chromosome. We discuss genome architecture and evolution and additional genes potentially involved in virulence and metabolic parasitism of host plants.
Asunto(s)
Agrobacterium tumefaciens/genética , Genoma Bacteriano , Análisis de Secuencia de ADN , Agrobacterium tumefaciens/clasificación , Agrobacterium tumefaciens/patogenicidad , Agrobacterium tumefaciens/fisiología , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/química , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Ciclo Celular , Cromosomas Bacterianos/genética , Replicación del ADN , Genes Bacterianos , Datos de Secuencia Molecular , Filogenia , Tumores de Planta/microbiología , Plantas/microbiología , Plásmidos , Replicón , Rhizobiaceae/genética , Transducción de Señal , Sinorhizobium meliloti/genética , Sintenía , Telómero , Virulencia/genéticaRESUMEN
Uncomplicated tubal pregnancies were treated by local injection of methotrexate (25 to 50 mg) and PGF2 alpha (5 to 10 mg). Trophoblastic resorption occurred in 50 (96.1%) of 52 patients within 20 days after treatment and without side effects. Three months after treatment, the affected tube was patent in 43 (91.4%) of 47 patients continuing in the study, and the subsequent intrauterine pregnancy rate was 40.4%. The combined use of methotrexate and PGF2 alpha appears to be a promising treatment for tubal pregnancy and should be evaluated further.