RESUMEN

OBJECTIVE: The roots of Chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. The aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from Chlorophytum borivilianum (ISCB). METHODS: The ISCB was screened using the carrageenan-induced paw edema, histamine-induced paw edema, cotton pellet-induced granuloma, and Freund's adjuvant-induced arthritis in rats at orally administered doses of 3, 10, and 30 mg/kg. Effect of ISCB on histone deacetylase (HDAC) level was measured by the HDAC assay at the highest dose (30 mg/kg). RESULTS: The results showed that the ISCB significantly reduced carrageenan-induced inflammation, histamine-induced inflammation, cotton pellet-induced granuloma and Freund's adjuvant-induced arthritis in rats. The ISCB at a dose of 30 mg/kg significantly inhibited HDAC level in rat paw tissue. CONCLUSION: It is concluded that saponins isolated from roots of C. borivilianum possess anti-inflammatory and anti-arthritic properties. ISCB may act by inhibiting histamine, prostaglandin and HDAC. This suggests that ISCBs have potential for therapeutic use in the treatment of inflammation and arthritis.

Asunto(s)

Antiinflamatorios/farmacología , Artritis Experimental/tratamiento farmacológico , Inhibidores de Histona Desacetilasas/farmacología , Liliaceae/química , Saponinas/farmacología , Animales , Femenino , Histona Desacetilasas/metabolismo , Masculino , Raíces de Plantas/química , Ratas , Ratas Wistar , Saponinas/uso terapéutico , Saponinas/toxicidad

RESUMEN

CONTEXT: Solanum xanthocarpum Schrad and Wendl (Kantakari) is a diffuse herb with prickly stem, traditionally used for the treatment of inflammation and one in the group of dasamula (group of ten herbs) herbs commonly used drug in Ayurveda. AIMS: In continuation of search for potent natural anti-inflammatory agents, the present research work was planned to evaluate the anti-inflammatory activity of ethanol extract of S. xanthocarpum whole plant. SETTINGS AND DESIGN: The ethanol extract was evaluated at dose 10, 30 and 100 mg/kg p.o. in rats. MATERIALS AND METHODS: Using pharmacological screening models carrageenan induced rat paw edema, histamine induced rat paw edema and cotton pellet granuloma in rats. STATISTICAL ANALYSIS USED: Data obtained was analyzed statistically using analysis of variance followed by post-hoc Dunnett test, P < 0.05 is considered as statistically significant. RESULTS: Acute treatment didn't show anti-inflammatory activity against carrageenan and histamine induced paw edema. However, administration of 100 mg/kg p.o for 7 day reduced the granuloma formation in cotton pellet granuloma model. CONCLUSIONS: Present results support the traditional use of plant for anti-inflammatory activity. In brief, the results provide scientific pharmacological basis for the therapeutic use of S. xanthocarpum.