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Polygonum odoratum (= Persicaria odorata), known as rau ram or sang hum, is native to southeastern Asia and is a common herb in Vietnamese cuisine (1). It has been studied most extensively for its aromatic compound content (2). In Florida, rau ram commonly is grown hydroponically in greenhouses using large, cement beds with recirculated water. The plants form dense mats from which new growth is trimmed for market. During January of 2002, a severe dieback was observed in one production house in Saint Lucie County, FL. Plants with less severe symptoms were yellowed and stunted. Roots of symptomatic plants were largely decayed with root symptoms beginning as a tip necrosis. The cortex of severely affected roots slipped off easily, leaving a stringy vascular system. Plating of symptomatic tissue from 20 randomly selected plant samples was performed with multiple general and selective media including potato dextrose agar, corn meal agar with pimaricin, ampicillin, rifampicin, and pentachloronitrobenzene (PARP) (3). All colonies produced were identified as Pythium helicoides Drechsler on the basis of sporangial, oogonial, and antheridial characteristics (4). Isolates had proliferous, obovoid, papillate sporangia, and were homothallic with smooth-walled oogonia and thick-walled, aplerotic oospores. Multiple antheridial attachments per oogonium were common with the antheridium attached along its entire length. Pathogenicity tests were conducted using P. odoratum plants grown from commercial transplants. Two tests were performed. Each test was conducted using eight inoculated and eight control plants. In the first test, plants were maintained in 10-cm pots immersed in sterilized pond water for the duration of the test. Plants were inoculated with five 7- × 70-mm sections of freshly growing mycelial culture per plant using 10-day-old cultures of Pythium helicoides grown on water agar. Chlorosis was observed at approximately 2 months after inoculation. Root necrosis was observed in inoculated plants approximately 5 months after inoculation. This test was performed in the greenhouse with temperatures ranging from 20 to 30°C. The second test was performed in growth chambers at 35 to 40°C. Plants were maintained in 10-cm pots immersed in Hoagland's solution and were inoculated with four 6-mm plugs per plant. Symptoms were observed on inoculated plants at this temperature within 1 week of inoculation. No chlorosis or root decay was observed in noninoculated, immersed plants. The pathogen was reisolated from inoculated, symptomatic tissue. To our knowledge, this is the first report of root rot of P. odoratum caused by Pythium helicoides. References: (1) R. E. Bond. Herbarist 55:34, 1989. (2) N. X. Dung et al. J. Essent. Oil Res. 7:339, 1995. (3) M. E. Kannwischer and D. J. Mitchell. Phytopathology 68:1760, 1978. (4) A. J. van der Plaats-Niterink. Monograph of the Genus Pythium. Vol. 21, Studies in Mycology. Centraalbureau voor Schimmelcutltures, Baarn, The Netherlands, 1981.

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Increased inputs of phosphorus (P) and heavy metals to agricultural soils have caused considerable concern. Information on accumulation and chemical forms of the elements in soils is needed as a guide for the judicious application of agricultural chemicals and organic manures. The focus of this study was to assess accumulation of P and heavy metals among various fractions of a sandy soil with a 25 year history of vegetable crop production and primarily inorganic fertilization. The results demonstrated that long-term vegetable production practices changed concentrations and partitioning of P and heavy metals in the soil. Phosphorus, Cu, Zn, and Mn were significantly accumulated and moved downward along the soil profile. Most of the total Cr in the vegetable soil accumulated in the upper 0-15 cm. However, there was no significant accumulation and transport of Cd, Co, Mo, Ni, and Pb in the vegetable soil. Major P fractions in the vegetable soil were NaHCO3-P, followed by HCl-P and residual P. Copper, Zn, and Mn accumulated predominantly in the CaCO3 fraction or oxide fraction, whereas Cr accumulated mainly in the organically bound fraction, indicating that P, Cu, Zn, and Mn in the vegetable soil have greater mobility potential. Compared with adjacent forest soil, the vegetable soil had a lower percentage of P, Cu, Zn, and Mn in the residual fractions, and a higher percentage of P, Cu, Zn, and Mn in the CaCO3 fractions or organically bound fraction.

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Information on P release potential in relation to labile P and P fractions in sandy soils is limited. In this study, P release potential was determined by leaching, and labile P, soil P fractionation, and P adsorption capacity were measured in the laboratory using 96 Florida sandy soil samples to evaluate the relationship between P release in water and soil P status. The sandy soils had a very low P adsorption capacity. The adsorption maximum, as calculated from the Langmuir equation, averaged 40.4 mg P kg(-1). More than 10% of the soil P was water soluble, indicating a high risk of P leaching from soil to water. Successive leaching using deionized water released, on average, 7.7% of total P (144.5 mg kg(-1)) in different soils, whereas labile P recovered by successive water extraction accounted for 39.2% of the total P. Variation in release potential among the different soils could be explained more by the difference in amounts of extractable P than the adsorption capacity. Total amounts of P released by successive leaching were significantly correlated with all labile P indices measured by different methods and all soil P fractions except for residual P. The correlation coefficients (r) were 0.97** for water-soluble P, 0.96** for 0.01 M CaCl2-P, 0.94** for Olsen P, 0.86** for Mehlich 1-P, 0.77*** for Mehlich 3-P, and 0.64*** for Bray 1-P. There were no obvious turning points in the relationships between Olsen-P, water-soluble P, or CaCl2-P and the amounts of P released from the sandy soils. The release of P from the sandy soils appeared to be controlled by a precipitation-dissolution reaction rather than a P sorption-desorption process. Furthermore, the sequential extraction of soils using deionized water indicated that P released was not limited to the labile P (H2O-P, NaHCO3-IP) and potentially labile P (NaOH-P) pools, but also from the HCl-P, indicating that all of P fractions except for residual P in the sandy soils can contribute to P release.

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Pepper mild mottle virus (PMMoV) has been identified on pepper in Florida several times since 1997. Initial observations were on ornamental peppers (Capsicum chinense, C. frutescens, and C. annuum). However, in the winter growing seasons of 1999 and 2000, PMMoV was detected in several bell pepper (C. annuum) cultivars in commercial production fields in south Florida. Symptoms in bell pepper plants were observed to vary with plant age and cultivar, and included stunting of young plants and puckering and yellow mottling of leaves. Fruit was deformed (lumpy and mottled) and slightly reduced in size. Older fruit exhibited brown streaks and, in some cultivars, an undesirable color at maturity. Incidence in commercial bell pepper fields varied from <1 to 30%. Symptoms on mechanically inoculated indicator hosts, analysis of viral-associated double-stranded RNA, and inclusion body morphology suggested the presence of a tobamovirus. PMMoV was specifically identified by serological testing using ELISA (Agdia, Elkhart, IN). To our knowledge, this is the first definitive identification of PMMoV (2) in Florida, although a prior isolation of the same or a similar virus was made in 1964 and reported as the Samsun latent strain of Tobacco mosaic virus (1). The occurrence of this seedborne virus in an area of intensive commercial pepper production requires careful monitoring to avoid future significant losses. References: (1) W. H. Greenleaf et al. Phytopathology 54:1367, 1964. (2) C. Wetter. Plant Dis. 68:597, 1984.

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The Sw-5 locus confers dominant resistance to tomato spotted wilt virus (TSWV). To map the location and facilitate the identification of markers linked to Sw-5 we developed a pair of near-isogenic lines (NILs) and an F2 Lycopersicon esculentum x L. pennellii population segregating for resistance to TSWV. DNA from the NILs was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic DNA (RAPD) approach. One random primer (GAGCACGGGA) was found to produce a RAPD band of about 2200 bp that demonstrates linkage to Sw-5. Data from co-segregation of resistance and restriction fragment length polymorphisms (RFLPs) in a F2 interspecific population position Sw-5 between the markers CT71 and CT220 near the telomere of the long arm of chromosome 9.

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The developing avian cornea is enclosed within a nerve ring from which numerous neurites extend into the cornea as radially ingrowing, regularly bifurcating fascicles. The authors now assess the degree of positional specificity of developing corneal nerves by photographic comparison and digital computer signal averaging. Such analyses demonstrate that nerves display extensive homology of position within the developing nerve ring, indicating that their growth cones originally followed specific pathways around the cornea. During their extension within the cornea, nerve fascicles bifurcate only within distinct, successive, and concentric zones suggesting that the position of an intra-corneal nerve branch point is designated by the intra-corneal milieu. Since the distance between the branch zones reported here increases with corresponding corneal growth, neurite elongation may occur both at the growth cone and along the axon at inter-branch sites of extending corneal nerves.

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