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J Chromatogr B Biomed Sci Appl ; 718(2): 211-24, 1998 Nov 06.
Artículo en Inglés | MEDLINE | ID: mdl-9840431

RESUMEN

A multisteroid screening method has been developed based on the use of 1-[4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index standards and UV absorbance spectra recorded on-line with a diode-array detector using reversed-phase high-performance liquid chromatographic gradient elution with acetonitrile and water. The effect of chromatographic conditions on retention indices of steroids were studied. The method was tentatively applied to profiling of steroids in serum samples.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Esteroides/sangre , Femenino , Humanos , Estructura Molecular , Estándares de Referencia , Espectrofotometría Ultravioleta
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