RESUMEN
Lutein is widely used in medicine, health care, and food processing due to its antioxidant effects; however, it is difficult for the traditional extraction of lutein using marigolds to meet the increasing market demand for lutein. To achieve high-efficiency lutein production, we investigated the effects of different conditions on the biomass accumulation and lutein yield of Chlorella vulgaris. The optimized cultivation conditions include mixotrophic cultivation using sodium nitrate as a nitrogen source, maintaining a total-organic-carbon-to-total-nitrogen ratio of 12:1, a total-nitrogen-to-total-phosphorus ratio of 10:1, and lighting duration of 24 h. The results of the study indicated that under these specific conditions, Chlorella vulgaris attained a final biomass concentration, biomass productivity, and growth yield of 6.08 g·L-1, 1.00 g·L-1·d-1, and 1.67 g biomass/g TOC, respectively. Additionally, the concentrations of total chlorophyll, carotenoid, lutein, and protein reached 139.20 mg·L-1, 31.87 mg·L-1, 15.02 mg·L-1, and 2.17 g·L-1, respectively, and the content of lutein reached 2.47 mg·g-1. This study supplies a theoretical basis for the industrial application of lutein production using Chlorella vulgaris.
RESUMEN
Cold atmospheric pressure plasma (CAPP) technique is an innovative non-thermal approach for food preservation and decontamination. This study aimed to evaluate the effect of CAPP power density on microorganism inactivation and quality of Spirulina platensis (S. platensis) slurry. 91.31 ± 1.61% of microorganism were inactivated within 2.02 ± 0.11 min by 26.67 W/g CAPP treatment under 50 â. Total phenolic, Chlorophyll-a (Chl-a), and carotenoids contents were increased by 20.51%, 63.55%, and 70.04% after 20.00 W/g CAPP treatment. Phycobiliproteins (PBPs), protein, intracellular polysaccharide, and moisture content of S. platensis was decreased, while vividness, lightness, color of yellow and green, antioxidant activity, Essential Amino Acid Index were enhanced after CAPP treatment. The nutrient release and filaments breakage of CAPP-treated S. platensis improved its bio-accessibility. The findings provided a deep understanding and insight into the influence of CAPP treatment on S. platensis, which were meaningful for optimizing its sterilization and drying processing condition.