RESUMEN

Proteomics and metabolomics technologies are potentially useful tool for the study of the very complex process of cell adaptation to protein-free medium. In this work, we used the iTRAQ technology to analyze different protein levels in adapted and non-adapted NS0 myeloma cell line. Several proteins with differential expression profile were characterized and quantified. Carbohydrate metabolism, protein synthesis and membrane transport were the principal pathways that change after the adaptation. Changes in lactate production rate with respect to glucose consumption rate were observed according to the changes observed by proteomic.

Asunto(s)

Técnicas de Cultivo de Célula/métodos , Mieloma Múltiple/metabolismo , Proteoma , Adaptación Fisiológica , Animales , Anticuerpos Monoclonales , Línea Celular Tumoral , Medio de Cultivo Libre de Suero , Receptores ErbB/antagonistas & inhibidores , Humanos , Insulina , Ratones , Proteómica