RESUMEN
OBJECTIVES: To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico). DESIGN AND METHODS: 258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU. RESULTS: A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1. CONCLUSIONS: These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease.
Asunto(s)
Enfermedad de Chagas/sangre , Enfermedad de Chagas/epidemiología , Trypanosoma cruzi/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Antiprotozoarios/sangre , Western Blotting , Enfermedad de Chagas/parasitología , Ensayo de Inmunoadsorción Enzimática/métodos , Femenino , Humanos , Masculino , México , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Valor Predictivo de las Pruebas , Proyectos de Investigación , Sensibilidad y Especificidad , Estudios Seroepidemiológicos , Pruebas Serológicas , Población Suburbana , TemperaturaRESUMEN
Although human leishmaniasis has been reported in 20 states in Mexico, no case of leishmaniasis has been reported in cats to date. In the Yucatan Peninsula, it has been found that dogs may act as reservoirs for at least three Leishmania species (Leishmania mexicana, Leishmania braziliensis, and Leishmania panamensis). In this study we identified specific antibodies against these three Leishmania spp. and Trypanosoma cruzi in the sera from 95 cats from two States on the Yucatan Peninsula, namely Quintana Roo and Yucatan, by ELISA and Western blot techniques using whole extract and an iron superoxide dismutase excreted by the parasites as antigens. As well as demonstrating the presence of trypanosomatid antibodies in the feline population on the Yucatan Peninsula, we were also able to confirm the high sensitivity and specificity of the iron superoxide dismutase antigen secreted by them, which may prove to be very useful in epidemiological studies.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Enfermedades de los Gatos/inmunología , Enfermedad de Chagas/veterinaria , Leishmania/inmunología , Leishmaniasis/veterinaria , Superóxido Dismutasa/inmunología , Trypanosoma cruzi/inmunología , Animales , Anticuerpos Antiprotozoarios/inmunología , Antígenos de Protozoos/inmunología , Western Blotting , Enfermedades de los Gatos/epidemiología , Enfermedades de los Gatos/parasitología , Gatos , Enfermedad de Chagas/epidemiología , Enfermedad de Chagas/inmunología , Enfermedad de Chagas/parasitología , Ensayo de Inmunoadsorción Enzimática , Leishmania/aislamiento & purificación , Leishmania/patogenicidad , Leishmaniasis/epidemiología , Leishmaniasis/inmunología , Leishmaniasis/parasitología , México/epidemiología , Carga de Parásitos , Prevalencia , Sensibilidad y Especificidad , Trypanosoma cruzi/aislamiento & purificación , Trypanosoma cruzi/patogenicidadRESUMEN
Leishmania spp. has been recorded in humans and in dogs, and numerous studies have demonstrated that dogs act as reservoirs for visceral leishmaniasis. The objective of this study was to determine the prevalence of three species of the Leishmania genus and possible associated factors in sera of 218 dogs from two different populations in Mérida, Yucatán (Mexico). The sera were analyzed to detect antibodies against L. mexicana, L. braziliensis, and L. infantum using the superoxide dismutase- enzyme-linked immunosorbent assay (SOD-ELISA) and Western blot as confirmation. The Fe-SOD excreted was used as the antigenic fraction for the three Leishmania species. The prevalence values found were 30.2% (L. mexicana), 8.2% (L. braziliensis), and 11.9% (L. infantum), with L. mexicana seroprevalence being greater than L. braziliensis and L. infantum (p<0.05). Five percent (11/218) of the dogs showed antibodies against L. mexicana/L. braziliensis, 5.5% (12/218) with L. mexicana/L. infantum and 1.8% (4/218) with L. mexicana/L. braziliensis/L. infantum. No relationship (p>0.05) was found between antibodies against L. mexicana and breed, age, physical condition, or cutaneous lesions in dogs. This study provides evidence of antibodies against L. mexicana, L. braziliensis and L. infantum in dog populations from Mérida, Mexico.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/inmunología , Leishmania braziliensis/inmunología , Leishmania infantum/inmunología , Leishmania mexicana/inmunología , Leishmaniasis/veterinaria , Animales , Western Blotting , Perros , Ensayo de Inmunoadsorción Enzimática , Leishmaniasis/inmunología , Masculino , México/epidemiología , PrevalenciaRESUMEN
The in vitro and in vivo trypanocidal activities of nine flavonoids (1-9) isolated from the aerial parts of Delphinium staphisagria have been studied in both the acute and chronic phases of Chagas disease. The antiproliferative activity of these substances against Trypanosoma cruzi (epimastigote, amastigote, and trypomastigote forms) in some cases exhibited more potent antitrypanosomatid activity and lower toxicity than the reference drug, benznidazole. Studies in vitro using ultrastructural analysis together with metabolism-excretion studies were also performed in order to identify the possible action mechanism of the compounds tested. Alterations mainly at the level of the mitochondria may explain metabolic changes in succinate and acetate production, perhaps due to the disturbance of the enzymes involved in sugar metabolism within the mitochondrion. In vivo studies provided results consistent with those observed in vitro. No signs of toxicity were detected in mice treated with the flavonoids tested, and the parasitic charge was significantly lower than in the control assay with benznidazole. The effects of these compounds were also demonstrated with the change in the anti-T. cruzi antibody levels during the chronic stage.
Asunto(s)
Enfermedad de Chagas , Delphinium/química , Flavonoides/aislamiento & purificación , Flavonoides/farmacología , Tripanocidas , Animales , Enfermedad de Chagas/sangre , Enfermedad de Chagas/tratamiento farmacológico , Enfermedad de Chagas/inmunología , Enfermedad de Chagas/parasitología , Chlorocebus aethiops , Flavonoides/química , Ratones , Mitocondrias/metabolismo , Estructura Molecular , Pruebas de Sensibilidad Parasitaria , Tripanocidas/sangre , Tripanocidas/química , Tripanocidas/inmunología , Tripanocidas/aislamiento & purificación , Tripanocidas/farmacología , Trypanosoma cruzi/efectos de los fármacos , Células VeroRESUMEN
An increasing number of studies have reported high infection rates for American cutaneous leishmaniasis in dogs, which have thus been proposed as the reservoir host. Canine leishmaniasis is widespread in different states in Mexico, where a number of Leishmania species have been isolated from dogs. In the present study, the detection of different Leishmania species is described in stray dogs from two localities, namely Tulum and Celestún on the Yucatan Peninsula (Mexico). The use of iron-superoxide dismutase excreted by the parasites as the antigen fraction and enzyme-linked immunosorbent assay and western blot tests allowed us to confirm the presence of at least three species of Leishmania (Le. mexicana, Le. braziliensis, and Le. panamensis), some of which are reported for the first time in this species. In addition to a high prevalence of Le. mexicana and Le. braziliensis, and to a lesser degree, Le. panamensis, there is a significant prevalence of Trypanosoma cruzi, suggesting that the dog may be a source of transmission of trypanosomiasis. However, a more thorough epidemiological study on the dog population, both wild as well as urban, of the Yucatan Peninsula will be required to design a control strategy for these diseases, paying particular attention to the population affected and even broadening the study to other Mexican states as well as neighboring countries. These results again confirm that iron-superoxide dismutase excreted by the different trypanosomatid species constitutes a good source of antigen for serodiagnosis in epidemiological studies.