RESUMEN
Rapid and sensitive high-pressure liquid chromatographic (HPLC) assays for ticarcillin in serum and urine have been developed. Sample pretreatment and optimized chromatographic conditions are presented for a C-18-bonded reversed-phase column used in an internal standard assay method. Ticarcillin has a retention time of or approximately 5.3 min at a flow rate of 1.5 ml/min for a mobile phase of acetonitrile-aqueous 0.06 M sodium biphosphate, pH 2.05, (50.5:100). In a two-step extraction procedure, the ticarcillin extraction efficiencies from serum and urine were 76.1 +/- 4.7 and 80.9 +/- 3.2% respectively. The assay sensitivity limit for ticarcillin in these fluids is approximately 1.0 microgram/ml. A comparison is made of the HPLC and microbiological assay results for ticarcillin in 20 different but equally divided serum samples obtained from two volunteers.
Asunto(s)
Penicilinas/análisis , Ticarcilina/análisis , Bacterias/efectos de los fármacos , Cromatografía Líquida de Alta Presión/métodos , Humanos , Concentración de Iones de Hidrógeno , Ticarcilina/sangre , Ticarcilina/orinaRESUMEN
Two rapid, specific, and sensitive high-pressure liquid chromatographic (HPLC) assays were developed for cloxacillin in serum and urine. A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficiencies are reported from serum and urine. Equations are presented for linear relationships between peak height or peak area ratios of cloxacillin to nafcillin (internal standard) and the cloxacillin concentration over a range of 0-80 micrograms/ml. The sensitivity limit of these assays was approximately 0.3 microgram/ml of a standard solution for one method and 0.05 microgram /ml for the other HPLC assay.