RESUMEN
During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of strains isolated from the two positive stool samples were negative for Stxs-production in Vero cytotoxicity assay and by PCR.
Asunto(s)
Diarrea/diagnóstico , Heces/química , Toxina Shiga I/metabolismo , Toxina Shiga II/metabolismo , Adolescente , Adulto , Animales , Anticuerpos Monoclonales/inmunología , Niño , Preescolar , Chlorocebus aethiops , Escherichia coli/metabolismo , Escherichia coli/patogenicidad , Humanos , Lactante , Pruebas de Neutralización , Toxina Shiga I/inmunología , Toxina Shiga I/toxicidad , Toxina Shiga II/inmunología , Toxina Shiga II/toxicidad , Células VeroRESUMEN
Shiga-like toxin-producing (SLTEC) Escherichia coli strains are one of the most important food borne emerging pathogens. One hundred and fifty-seven E. coli strains isolated from 39 children with diarrhea of unknown origin and one hundred and five E. coli strains from 20 healthy children were examined for Shiga-like toxin production in Vero cell line assay. The synthesis of Shiga-like toxin was observed on Vero cell line and confirmed by PCR for one of 262 E. coli strains tested. The shiga-like toxin II-positive E. coli strain was isolated from 2-years old healthy child with no symptoms of gastrointestinal tract infection.
Asunto(s)
Diarrea/microbiología , Escherichia coli/aislamiento & purificación , Heces/microbiología , Proteínas Hemolisinas/metabolismo , Toxina Shiga II/metabolismo , Animales , Línea Celular , Preescolar , Chlorocebus aethiops , ADN Bacteriano/análisis , Escherichia coli/genética , Escherichia coli/fisiología , Infecciones por Escherichia coli/microbiología , Humanos , Células VeroRESUMEN
During 5 y (1997-2002) in the region of Lower Silesia, Poland, 55 shiga-like toxin-producing strains were isolated from children with diarrhoea, none of whom developed haemolytic uremic syndrome. Shiga-like toxin production was detected on Vero cells and confirmed by the detection of slt1 and slt2 gene sequences. Most of isolates examined possessed slt2 gene alone (41.8%), or in combination with slt1 gene (38.2%). None of the shiga-like toxin-producing strains was of serotype O157:H7. It appears that non-O157 shiga-like toxin-producing E. coil strains are prevalent as the causal agent of severe diarrhoea in the region.