RESUMEN
In this study, the complete mitochondrial genomes of five noctuoid moth species, namely, Paectes cristatrix, Hemichloridia euprepia, Trigonodes hyppasia, Mythimna unipuncta, and Risoba obstructa, were newly sequenced and characterized. Based on our investigations, the five mitogenomes showed sizes of 15409, 15619, 15654, 15403, and 15233 bp, respectively, and encoded 37 genes (13 protein-coding, 2 ribosomal RNA, and 22 transfer RNA genes) and a control region. All protein-coding genes (PCGs) in these mitogenomes had typical ATN start codons except for cox1 and nad1. The non-canonical codon CGA was the starter in H. euprepia and M. unipuncta and TTG was the starter in T. hyppasia for cox1. The unusual codon GTG was the starter in R. obstructa for nad1. All tRNA genes showed distinctive putative cloverleaf structures except trnS1 (AGN), which lacked the dihydrouridine (DHU) stem. Bayesian inference (BI) and maximum likelihood (ML) analyses were employed based on 13 PCGs using 159 taxa among the five families of the superfamily Noctuoidea, and the results showed the monophyly of this superfamily with well-supported values. Phylogenetic analyses showed that the newly sequenced endemic species Hemichloridia euprepia clustered separately along with the subfamilies Cuculliinae and Acronictinae with fairly supported values.
Asunto(s)
Genoma Mitocondrial , Mariposas Nocturnas , Animales , Mariposas Nocturnas/genética , Genoma Mitocondrial/genética , Filogenia , Teorema de Bayes , ARN de Transferencia/genética , Codón/genéticaRESUMEN
The species Eudocima salaminia (Cramer, 1777) commonly known as the fruit-piercing moth belongs to family Erebidae. Its distribution varies from India and across South-east Asia, pacific islands and parts of Australia. The insect is a devastating pest of citrus, longans and lychees. In the present study, complete mitochondrial genome of Eudocima salaminia was sequenced and analyzed using Illumina sequencer. The phylogenetic tree was reconstructed based on nucleotide sequences of 13 PCGs using Maximum likelihood method-General Reversible mitochondrial (mtREV) model. The mitogenome has 15,597 base pairs (bp) in length, comprising of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and A + T-rich region. All protein-coding genes (PCGs) initiate with canonical start codon ATN. The gene order (trnQ-trnI-trnM) of tRNA shows a different rearrangement compared to ancestral insect gene order (trnI-trnQ-trnM). Almost all tRNAs have a typical cloverleaf secondary structure except for trnS1 (AGN) which lacks the dihydrouridine arm. At the beginning of the control region, we observed a conserved polyT", motif "ATTTA" and microsatellite (TA)n element. There are 21 intergenic regions and five overlapping regions ranging from 1 to 73 bp and 1 to 8 bp, respectively. The phylogenetic relationships based on nucleotide sequences of 13 PCGs using Maximum likelihood method showed the family level relationships as (Notodontidae + (Euteliidae + Noctuidae + (Erebidae + Nolidae))). The present study represents the similarity to phylogenetic analysis of Noctuoidea mitogenome. Moreover, the family Erebidae is the sister to the families of (Euteliidae + Noctuidae + Nolidae).