RESUMEN
Most lumbar intradural schwannomas present initially as radiculopathies with sensory disturbances. However, neurogenic bladder dysfunction may be one of the earliest manifestations and can cause long-term disability. We present the case of a patient with a L3-4 schwannoma (newly diagnosed owing to recurrent urinary retention and urinary tract infection) who finally underwent surgical resection. Improvement of bladder sensation was documented by urodynamic study and the patient was subsequently weaned off her Foley catheter with satisfactory outcome.
Asunto(s)
Neurilemoma/cirugía , Neoplasias de la Médula Espinal/cirugía , Vejiga Urinaria Neurogénica/cirugía , Cateterismo Urinario , Retención Urinaria/cirugía , Anciano de 80 o más Años , Cistografía , Femenino , Gadolinio/administración & dosificación , Humanos , Laminectomía/instrumentación , Laminectomía/métodos , Dolor de la Región Lumbar/etiología , Dolor de la Región Lumbar/cirugía , Vértebras Lumbares , Imagen por Resonancia Magnética/métodos , Neurilemoma/complicaciones , Neurilemoma/diagnóstico por imagen , Recurrencia , Neoplasias de la Médula Espinal/complicaciones , Neoplasias de la Médula Espinal/diagnóstico por imagen , Vejiga Urinaria Neurogénica/diagnóstico por imagen , Vejiga Urinaria Neurogénica/etiología , Retención Urinaria/diagnóstico por imagen , Retención Urinaria/etiología , Infecciones Urinarias/etiología , UrodinámicaRESUMEN
We present an extremely rare case of delayed and combined ventriculoperitoneal shunt blockage, viscus perforation and migration into the urethra manifested by a repeated urinary tract infection. This was discovered six months after the shunt was inserted. Although there were various other transient symptoms, the patient did not show obvious peritoneal signs. This complication could have been lethal if the discovery had been delayed. One of the best ways of preventing such migration is possibly the use of a softer catheter. However, making sure of appropriate redundancy for the abdominal part of the catheter may be of equal importance.
Asunto(s)
Migración de Cuerpo Extraño/complicaciones , Complicaciones Posoperatorias/etiología , Uretra , Infecciones Urinarias/etiología , Derivación Ventriculoperitoneal , Femenino , Migración de Cuerpo Extraño/diagnóstico por imagen , Humanos , Hidrocefalia/cirugía , Complicaciones Posoperatorias/diagnóstico por imagen , Tomografía Computarizada por Rayos X , VíscerasRESUMEN
Two isoenzymes of cAMP-dependent protein kinases were found in MCF-7 cytosol. The regulatory (cAMP-binding) subunit of protein kinase I (predominant form) has an apparent molecular weight (MW) of 49,000 and the two forms of regulatory subunits of protein kinase II have MWs of 52,000 and 54,000. Substantial amounts of the 49,000 protein cochromatographed on DEAE cellulose with protein kinase II. The quantities of protein kinase holoenzyme activity are strongly influenced by extraction procedures: the use of EDTA and of the protease inhibitor benzamidine can lead to extensive dissociation. On the other hand, high yields of cAMP-dependent protein kinase holoenzyme activity were consistently obtained with 150 mM KCl.
Asunto(s)
Neoplasias de la Mama/análisis , Proteínas Portadoras/análisis , Proteína Receptora de AMP Cíclico , Isoenzimas/análisis , Proteínas de Neoplasias/análisis , Proteínas Quinasas/análisis , Línea Celular , Cromatografía DEAE-Celulosa , Citosol/análisis , Femenino , Humanos , Peso MolecularRESUMEN
A technique for reproduction and quantitative determination of human cellular retinoic acid-binding protein (CRABP) activity in breast tissue specimens is described. A multiphasic polyacrylamide disc gel electrophoresis system (operative at pH 10.2) was adapted for this purpose. This technique allows, after incubation with tritiated retinoic acid (RA) overnight, the separation of the specific CRABP activity from the nonspecific serum-originated binding activity and from the free RA. Previous purification of the tissue cytosols is therefore not necessary. The same assay method was also used for the determination of the molecular weight (Ferguson plot, m.w. 13,000) and the dissociation constant Kd (2.5 x 10(-7) M) of mammary CRABP. The activity in tissue cytosol, stored at -70 degrees, was found to be stable for at least 3 months. Results from 88 breast tissue specimens of different pathological degree are presented. CRABP activity was found in all tissue categories with progressively increasing amounts from normal tissue to breast cancer. The activity in the cancer tissues (14.85 +/- 12.05 pmol RA bound per mg soluble protein: N = 27) was significantly different (p less than 0.001) from the activity determined in tissue with simple dysplasia without epithelial proliferation [4.3 +/- 2.2 (S.D.) pmol RA bound per mg protein; N = 30]. It is possible that in the cases where high amounts of CRABP activity are found in dysplastic and preneoplastic tissue, a high risk for breast cancer development exists. Therefore, CRABP is tentatively proposed as a dedifferentiation and/or proliferation marker.