RESUMEN
The reproducibility of laboratory methods, recommended by WHO and used by manufacturing and control institutions of most countries for the evaluation of the immunogenic potency of the pertussis, diphtheria and tetanus components of adsorbed DPT vaccine in the process of its production, was studied. This immunogenic potency was experimentally studied in comparison with the immunogenic potency of national standard samples from Bulgaria, Czechoslovakia, Hungary, Poland and Russia. The results of this study indicated that the coded commercial lots of adsorbed DPT vaccine corresponded to WHO requirements with respect to the immunogenic potency of all their components. At the same time, according to data provided by different laboratories, the indices of their immunogenic potency varied within rather wide limits. Taking into account the necessity of dosing all components of adsorbed DPT vaccine by immunizing (protective) units, the reliability of the recommended control methods should be enhanced by the maximum standardization of all testing conditions and the organization of periodic workshops on the basis of the leading WHO laboratory with a view to improving the qualification of specialists carrying out the quality control of commercial lots of adsorbed DPT vaccine.
Asunto(s)
Vacuna contra Difteria, Tétanos y Tos Ferina/inmunología , Vacuna contra Difteria, Tétanos y Tos Ferina/normas , Adsorción , Animales , Animales de Laboratorio , Bulgaria , República Checa , Evaluación Preclínica de Medicamentos/métodos , Evaluación Preclínica de Medicamentos/normas , Hungría , Polonia , Reproducibilidad de los Resultados , Federación de Rusia , Organización Mundial de la SaludRESUMEN
The immunocorrective effect of antistaphylococcal adsorbent prepared on the basis of silochrome, a silica matrix, has been revealed in experiments on peripheral blood lymphocytes obtained from 18 donors. The expression of receptors on human immunoregulating cells has been suppressed by the addition of staphylotoxin at a toxic concentration into the system. The experiments have shown that immunosorption is capable of extracting the toxin from the solution to a considerable degree, thus preventing the suppression of the receptor apparatus of immunoregulating lymphocytes. Staphylotoxin has been found capable of forming complexes with plasma ingredients, which increases the adsorption capacity of the immunosorbent.
Asunto(s)
Adyuvantes Inmunológicos , Toxinas Bacterianas/aislamiento & purificación , Inmunoadsorbentes , Dióxido de Silicio , Staphylococcus aureus/inmunología , Adsorción , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Toxinas Bacterianas/farmacología , Humanos , Técnicas de Inmunoadsorción , Inmunosupresores/farmacología , Indicadores y Reactivos , Receptores Inmunológicos/efectos de los fármacos , Receptores Inmunológicos/inmunología , Gel de Sílice , Soluciones , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunología , Factores de TiempoRESUMEN
The action of native staphylotoxin has been tested on T lymphocytes obtained from 21 healthy human donors. As revealed in this investigation, toxic action affects Fc-receptors to IgG, IgM and IgA. The dose-dependent effect of the preparation suggests its direct contacts with Fc-receptors of lymphocytic membranes. High doses of the toxin suppress the expression of Fc-receptors 6- to 12-fold, low doses of the toxin are capable of stimulating the expression of Fc gamma-receptors.