RESUMEN
Experimental studies of the mussel hydrolyzate (MIGI-K) have shown that this preparation can enhance both general and radiation resistance of the organism. Moreover, MIGI-K promotes elimination of incorporated radionuclides from the organism. Some properties of this preparation, in particular, the absence of toxicity or harmful side effects, have made it possible to apply MIGI-K as an adaptogen, that is, a food supplement oftherapeutic and preventive action used to increase radionuclide excretion in the Chernobyl emergency clean-up workers.
Asunto(s)
Hidrolisados de Proteína/farmacología , Tolerancia a Radiación/efectos de los fármacos , Protectores contra Radiación/farmacología , Animales , HumanosRESUMEN
We investigated the glucocorticoid level in plasma and adrenal glands of pregnant rats in the period of early organogenesis. Tests were performed 24 hours after stress of different etiology (acute hypobaric hypoxia, intermittent normobaric hypoxia, and immobilization) and then repeated in the adult offspring. There was a significant decrease in the glucocorticoid level in pregnant rats 24 hours after hypoxic stress. Various changes of the basal glucocorticoid level were found in the offspring after antenatal stress. Changes were mostly found in female offspring.
Asunto(s)
Glándulas Suprarrenales/metabolismo , Glucocorticoides/sangre , Estrés Fisiológico , Animales , Femenino , Hipoxia/sangre , Inmovilización/efectos adversos , Masculino , Embarazo , Ratas , Caracteres SexualesRESUMEN
The effect of immunocortin, an ACTH-like decapeptide VKKPGSSVKV corresponding to the 11-20 sequence of the variable part of the human IgG1 heavy chain on the content of 11-hydroxycorticosteroids (CS) in rat adrenal glands and blood serum in vivo was studied. An intramuscular injection of immunocortin at a dose of 10 microg/kg was found in an hour to induce a twofold decrease in CS content in the adrenal glands and a 1.8-fold increase in the blood serum CS content. At the same time, an immunocortin dose of 100 microg/kg exerted practically no effect on the CS content and its dose of 1000 microg/kg increased the CS content both in adrenal glands and in blood serum by 1.6 and 2.2 times, respectively. Four hours after the injection of any of the three doses of immunocortin, the CS content in adrenal glands did not differ from the control value, and after 24 h the content decreased threefold. Immunocortin was shown to be bound by the ACTH receptors in the membranes of the rat adrenal cortex with a high affinity and specificity (inhibiting the specific binding of 125I-labeled ACTH-(11-24) peptide with Ki of 1.2 nM).
Asunto(s)
11-Hidroxicorticoesteroides/metabolismo , Corteza Suprarrenal/metabolismo , Hormona Adrenocorticotrópica/química , Inmunoglobulina G/química , Inmunoglobulina G/farmacología , Región Variable de Inmunoglobulina/química , Fragmentos de Péptidos/farmacología , 11-Hidroxicorticoesteroides/sangre , Corteza Suprarrenal/citología , Corteza Suprarrenal/efectos de los fármacos , Secuencia de Aminoácidos , Animales , Cosintropina/administración & dosificación , Cosintropina/farmacología , Inmunoglobulina G/administración & dosificación , Técnicas In Vitro , Radioisótopos de Yodo , Masculino , Datos de Secuencia Molecular , Fragmentos de Péptidos/administración & dosificación , Fragmentos de Péptidos/química , Ratas , Ratas Wistar , Receptores de Corticotropina/metabolismoRESUMEN
It was shown that the addition of synthetic six-membered peptide (HLDF-6) and its Tyr-analog (HLDF-Y) to cultural medium significantly increased the survival of cells HL-60, treated by cold shock. The prophylactic administration of HDLF-Y (1 mg/kg, 4 hours prior to applied actions) decreased the response of hypothalamushypophysis-adrenal glands system and sympathicoadrenal system of rat males on supercooling and also increased the resistance of mouse males to supercooling and X-irradiation. In the experiences with females HDLF-Y did not show the similar biological activity.
Asunto(s)
Corticoesteroides/análisis , Monoaminas Biogénicas/análisis , Células HL-60/efectos de la radiación , Proteínas de Neoplasias , Fragmentos de Péptidos/farmacología , Tolerancia a Radiación , Adaptación Fisiológica , Glándulas Suprarrenales/química , Animales , Apoptosis , Diferenciación Celular , Supervivencia Celular , Frío , Medios de Cultivo , Interpretación Estadística de Datos , Perros , Epinefrina/análisis , Femenino , Fluorometría , Células HL-60/efectos de los fármacos , Humanos , Masculino , Espectrometría de Masas , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Norepinefrina/análisis , Dosis de Radiación , Radiación Ionizante , Protectores contra Radiación , Ratas , Ratas Wistar , Serotonina/análisis , Factores Sexuales , Bazo/química , Factores de TiempoRESUMEN
The unbalance in system histamine-diaminooxydase in myocardium of rats subjected to various stress actions (intensive cold influence, hyperthermia and hypoxia) was found. The preventive application of a natural radioprotector carnosine prevented apparent changes, that gives the basis to assume about carnosine ability to enlarge a nonspecific resistance of an organism.
Asunto(s)
Amina Oxidasa (conteniendo Cobre)/metabolismo , Carnosina/farmacología , Histamina/metabolismo , Miocardio/metabolismo , Animales , Hipoxia , Masculino , Protectores contra Radiación/farmacología , Ratas , TemperaturaRESUMEN
LPO products were measured in plasma and biogenic amines (serotonin, adrenalin, noradrenalin) in tissues of rats in different periods after hemorrhagic shock provoked by taking blood and maintenance of arterial pressure at the level of 40 mm Hg for 1 hour. Resuscitation was conducted by administration of autoblood. It was found that splenic serotonin levels decreased on experiment day 7 and went up on day 28. On late experiment stages noradrenalin levels in the adrenals were high. Early after resuscitation the trend was noted to higher LPO products concentration in plasma and serotonin in the brain stem. Intravenous injection of semax prevented serotonin fall in the spleen on experiment day 7. It is suggested that biogenic amines, especially serotonin system, are involved in mechanisms of postresuscitation disorders, in cerebral defects in particular, through prolongation of secondary hypoxia early after hemorrhagic shock and activation of hypothalamo-hypophyso-adrenal system late after the shock.
Asunto(s)
Glándulas Suprarrenales/metabolismo , Hormona Adrenocorticotrópica/análogos & derivados , Aminas Biogénicas/metabolismo , Tronco Encefálico/metabolismo , Fármacos Neuroprotectores/uso terapéutico , Fragmentos de Péptidos/uso terapéutico , Resucitación , Choque Hemorrágico/metabolismo , Bazo/metabolismo , Glándulas Suprarrenales/química , Glándulas Suprarrenales/efectos de los fármacos , Hormona Adrenocorticotrópica/uso terapéutico , Animales , Aminas Biogénicas/análisis , Química Encefálica/efectos de los fármacos , Tronco Encefálico/química , Tronco Encefálico/efectos de los fármacos , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Peroxidación de Lípido/efectos de los fármacos , Lípidos/sangre , Masculino , Ratas , Resucitación/métodos , Choque Hemorrágico/terapia , Bazo/química , Bazo/efectos de los fármacos , Factores de TiempoAsunto(s)
Tronco Encefálico/metabolismo , Catecolaminas/metabolismo , Hemorragia/metabolismo , Hipoxia/metabolismo , Neuropéptidos/farmacología , Hormona Adrenocorticotrópica/análogos & derivados , Hormona Adrenocorticotrópica/farmacología , Animales , FMRFamida/farmacología , Masculino , Fragmentos de Péptidos/farmacología , Ratas , Hormona Liberadora de Tirotropina/farmacologíaRESUMEN
X-irradiation of Micrococcus radiodurans cells with sublethal doses caused disturbances in the structure of a membrane-bound compact chromosome. Recovery of the compact chromosome occurred during the postirradiation incubation of the wild type cells and cells of the UVS-17 mutant deficient in DNA-polymerase. This process was blocked in cells of rec-30 mutant with the impaired system of genetic recombination: this is indicative of an important role played by rec-30 gene product in the postirradiation recovery of the compact chromosome in M. radiodurans cells.