RESUMEN
The effect of global system for mobile communication (GSM) radiofrequency fields on vascular permeability in the brain was studied using a purpose-designed exposure system at 898.4 MHz. Mice (n= 30) were given a single far field, whole body exposure for 60 minutes at a specific absorption rate of 4 W/kg. Control mice were also sham-exposed (n = 10) or permitted free movement in a cage (n = 10) to exclude any stress-related effects. Vascular permeability changes were detected using albumin immunohistochemistry and the efficacy of this vascular tracer was confirmed with a positive control group exposed to a clostridial toxin known to increase vascular permeability in the brain. No significant difference in albumin extravasation was detected between any of the groups at the light microscope level using the albumin marker.
Asunto(s)
Barrera Hematoencefálica/efectos de la radiación , Encéfalo/efectos de la radiación , Campos Electromagnéticos/efectos adversos , Traumatismos Experimentales por Radiación/etiología , Ondas de Radio/efectos adversos , Albúminas/análisis , Animales , Toxinas Bacterianas/farmacología , Encéfalo/irrigación sanguínea , Capilares/efectos de la radiación , Capilares/ultraestructura , Permeabilidad Capilar , Extravasación de Materiales Terapéuticos y Diagnósticos , Femenino , Técnica del Anticuerpo Fluorescente Indirecta , Ratones , Ratones Endogámicos C57BL , Traumatismos Experimentales por Radiación/patología , Teléfono/instrumentaciónRESUMEN
The efficacy of continuous low-dose xylazine infusion following an initial loading dose in providing analgesia in sheep was examined using an algesimetry method based on a leg lifting response to an electrical stimulus. Sheep received a 5 mg intramuscular injection of xylazine followed by continuous infusion of intravenous xylazine (2mg/h) for 90 min. This treatment resulted in significant increases in the level of current required to elicit a leg lifting response (287% of baseline) and steady state analgesia was maintained from 10 min after the start of the infusion until the end of the experimental period. This protocol appears to be a simple and effective regimen for providing steady state analgesia in sheep.
Asunto(s)
Agonistas alfa-Adrenérgicos/farmacología , Analgesia/veterinaria , Xilazina/farmacología , Agonistas alfa-Adrenérgicos/administración & dosificación , Analgesia/métodos , Animales , Inyecciones Intramusculares , Masculino , Ovinos , Xilazina/administración & dosificaciónRESUMEN
Amyloid precursor protein (APP) immunocytochemistry was used to study axonal and neuronal changes in guinea pig brains exposed to tunicamycin. Substantial axonal injury was found in ischemic-hypoxic foci and more generally, but this injury was not readily appreciable in conventionally stained sections. Neuronal perikaryal APP expression was also widely distributed, possibly as an acute phase response to this neurotoxin.
Asunto(s)
Precursor de Proteína beta-Amiloide/metabolismo , Antibacterianos/toxicidad , Axones/química , Química Encefálica , Cobayas/metabolismo , Neuronas/química , Tunicamicina/toxicidad , Animales , Axones/efectos de los fármacos , Encéfalo/efectos de los fármacos , Química Encefálica/efectos de los fármacos , Inmunohistoquímica/veterinaria , Neuronas/efectos de los fármacosRESUMEN
The recent high prevalence of fatal bites by Brown snakes (Pseudonaja genus) has led to this study of venom yields from 66 brown snake milkings over 15 months. The amount of venom obtained from all species was higher than reported previously. Electrophoretic and Western blotting analyses of their venoms showed significantly lower avidity of Brown snake antivenom (BS-AV) for the prothrombin activator (PA) component (190 kD) than for other venom components, including the neurotoxins. The LD50 of P. inframacula has been determined for the first time. SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) and Western blotting studies have shown that the Pseudonaja venoms contained proportionately more PA component than venoms of the Taipan (Oxyuranus scutellatus) or the Fierce snake (O. microlepidotus). Neutralization of the prothrombin activator of the Common Brown snake (P. textilis) (Pt-PA) by BS-AV was found to be time dependent and 40% remained unneutralized after 30 minutes incubation. Adult rats administered quantities of Pt-PA (IV) died with acute disseminated intravascular coagulation. Rats were made resistant to Pt-PA by preheparinization or by induction of tolerance to increasing quantities of Pt-PA. There is no evidence that Pt-PA has intrinsic toxicity apart from being a procoagulant. The improvement of BS-AV by addressing its deficiencies should be canvassed.
Asunto(s)
Antivenenos/uso terapéutico , Venenos de Serpiente/aislamiento & purificación , Venenos de Serpiente/toxicidad , Animales , Antivenenos/inmunología , Electroforesis en Gel de Poliacrilamida , Dosificación Letal Mediana , Masculino , Ratones , Ratones Endogámicos BALB C , Pruebas de Neutralización , Protrombina/aislamiento & purificación , Protrombina/metabolismo , Ratas , Ratas Sprague-Dawley , Venenos de Serpiente/inmunología , Especificidad de la EspecieRESUMEN
The analgesic action of intramuscularly injected buprenorphine, methadone, flunixin meglumine and xylazine was examined in sheep, using algesimetry based on a leg withdrawal response to an electrical stimulus. No analgesic response was detected for buprenorphine, methadone or flunixin meglumine. Only the alpha 2-adrenoceptor agonist, xylazine, produced an analgesic response. The current required to elicit a response increased by 170% (4.5 +/- 0.43 mA to 12.23 +/- 1.14 mA; mean +/- SE) after a dose of 0.05 mg/kg xylazine; by 180% (4.73 +/- 0.3 mA to 13.28 +/- 2.35 mA) after 0.1 mg/kg and by 510% (4.52 +/- 0.29 mA to 27.63 +/- 3.89 mA) after 0.2 mg/kg. Intramuscular xylazine appears to be an effective analgesic agent for acute pain in the sheep and further investigation into ideal administration regimens and dosage may provide more detailed information on relationships between dose, analgesic and sedative effects. The findings also suggest that some common analgesic agents, and opioids in particular, may be ineffective for the management of acute pain in sheep and that any analgesic should be administered only on the basis of its proven efficacy in that species.
Asunto(s)
Analgésicos/administración & dosificación , Dolor/veterinaria , Enfermedades de las Ovejas/prevención & control , Xilazina/administración & dosificación , Enfermedad Aguda , Animales , Femenino , Inyecciones Intramusculares/veterinaria , Masculino , Dolor/prevención & control , Ovinos , Resultado del TratamientoRESUMEN
An electroimmobilisation device has been developed to facilitate the automated shearing of sheep, but there is little information on its effects on the body. We have studied its effects on the cardiovascular system and on intermediary metabolism in sheep. Electroimmobilisation caused statistically significant increases in mean arterial pressure, heart rate, cardiac output, renal and hepatic and hindquarter glucose and lactate flux, organ and whole body oxygen flux, hindquarter blood flow and core temperature and decreases in arterial and posterior vena cava blood pH, renal and hepatic blood flow and PaCO2. Notably, no change occurred in PaO2. The metabolic changes demonstrated the capacity of sheep to respond to the increased muscular and cardiovascular work induced by electroimmobilisation. Pulmonary function was not compromised during electroimmobilisation as judged from blood gas changes, and the acid/base changes were rapidly reversed after electroimmobilisation. The recovery to control conditions for all perturbations generally took no longer than 30 min, consistent with a rapid and physiologically adequate reversal by the animal's homeostatic mechanisms.
Asunto(s)
Fenómenos Fisiológicos Cardiovasculares , Inmovilización/fisiología , Ovinos/fisiología , Equilibrio Ácido-Base , Animales , Presión Sanguínea , Temperatura Corporal , Dióxido de Carbono/sangre , Gasto Cardíaco , Estimulación Eléctrica , Femenino , Glucosa/metabolismo , Frecuencia Cardíaca , Lactatos/metabolismo , Oxígeno/sangre , Oxígeno/metabolismo , Flujo Sanguíneo RegionalRESUMEN
1. Choline production by various tissues of the sheep was measured by determining venous and arterial free choline concentrations in blood samples taken from various vessels in conscious multicannulated sheep. 2. Significant production of free choline occurred in the upper and lower body regions, and specifically in the heart, brain and hind-limb muscles of sheep, but there was no significant uptake or output of phosphatidylcholine across these tissues, as determined by arterio-venous differences. 3. In contrast, in the rat there were no significant arterio-venous differences in the concentrations of free choline or phosphatidylcholine across the hind-body. 4. Synthesis of phosphatidylcholine from endogenous phosphatidylethanolamine and S-adenosyl-L-[methyl-14C]methionine was measured in experiments in vitro using microsomal preparations from a variety of sheep and rat tissues. 5. The biosynthetic activity was highest in liver from sheep and rats, although the activity in sheep microsomal preparations was about one-quarter of that in rat microsomal preparations. 6. Microsomal preparations from sheep lung, kidney, gut epithelium, brain, heart and skeletal muscles also showed considerable biosynthetic activity, but in the rat the activity was virtually confined to the liver. 7. Overall, the results show a significant production of choline in extrahepatic tissues of the sheep, with skeletal muscle contributing some 60% of this extrahepatic activity. Thus the extrahepatic production of choline in the sheep, together with the extensive reutilization of bile choline, can explain the maintenance of the large endogenous body pool of choline in this species.
Asunto(s)
Colina/biosíntesis , Animales , Arterias/metabolismo , Colina/sangre , Femenino , Lípidos/sangre , Metilación , Microsomas/metabolismo , Fosfolípidos/metabolismo , Ratas , Ratas Endogámicas , Ovinos , Distribución Tisular , Venas/metabolismoRESUMEN
It has been proposed that acylation at the active site of plasmin is able to prevent its reaction with alpha 2-antiplasmin without affecting the fibrin affinity of the enzyme. To investigate the possibility that 99mTc-labelled acylplasmins are improved thrombus-detecting agents, six acylating agents were synthesised and their reaction with plasmin and the labelling of the products with 99mTc studies. Uptake of 99mTc-acylplasmins in an in vitro thrombus model was complicated by precipitation processes, which may in part account for the rapid blood clearance in rabbits and high liver uptake in mice injected with the compounds. Quantitative measurements using an in vivo rabbit thrombus model demonstrated that guanidinobenzoyl-plasmin exhibited nearly a threefold increase in thrombus uptake compared with non-acylated 99mTc-plasmin. The observed uptake is less than that obtained with 125I-fibrinogen at clinically useful time intervals post-injection but represents a significant advantage over the use of 99mTc-plasmin.
Asunto(s)
Compuestos de Organotecnecio , Trombosis/diagnóstico por imagen , Acilación , Animales , Femenino , Fibrinolisina/metabolismo , Humanos , Técnicas In Vitro , Tasa de Depuración Metabólica , Ratones , Conejos , Cintigrafía , Tecnecio/metabolismo , Trombosis/metabolismoRESUMEN
The effects of combining large doses of xylazine (1.1 mg per kg intravenously) with ketamine, methohexitone and thiopentone were studied in four experimental ponies. Onset of anaesthesia was more delayed after ketamine than after the barbiturates. Assessment of smoothness of induction and recovery indicated that all three combinations were effective and acceptable. Injection of xylazine was followed by atrioventricular (A-V) block which could be prevented by the prior administration of atropine. Blood pressure was well maintained with all three combinations of drugs. Arterial oxygen tension decreased as soon as the ponies became recumbent but there were no marked changes in arterial blood pH or carbon dioxide tension. Cardiac output was measured in one pony and was found to be least affected by ketamine. There was no great difference between the recumbency times after ketamine and methohexitone but thiopentone produced a significantly longer period of recumbency. In every instance the animals stood at the first attempt without struggling or excitement. The ability of the three drug combinations to produce surgically useful anaesthesia was not tested.