RESUMEN

Possible chemoprotective effects of the naturally occurring alkaloid boldine, a major alkaloid of boldo (Peumus boldus Mol.) leaves and bark, including in vitro modulations of drug-metabolizing enzymes in mouse hepatoma Hepa-1 cell line and mouse hepatic microsomes, were investigated. Boldine manifested inhibition activity on hepatic microsomal CYP1A-dependent 7-ethoxyresorufin O-deethylase and CYP3A-dependent testosterone 6 beta-hydroxylase activities and stimulated glutathione S-transferase activity in Hepa-1 cells. In addition to the known antioxidant activity, boldine could decrease the metabolic activation of other xenobiotics including chemical mutagens.

Asunto(s)

Antioxidantes/farmacología , Aporfinas/farmacología , Oxigenasas de Función Mixta/metabolismo , Preparaciones Farmacéuticas/metabolismo , Plantas Medicinales/química , Animales , Brasil , Línea Celular , Citocromo P-450 CYP1A1/antagonistas & inhibidores , Sistema Enzimático del Citocromo P-450/metabolismo , Glutatión Transferasa/metabolismo , Técnicas In Vitro , Ratones , Ratones Endogámicos C57BL , Microsomas Hepáticos/efectos de los fármacos , Microsomas Hepáticos/enzimología , Células Tumorales Cultivadas