RESUMEN
Halophiles, thriving in harsh saline environments, capture scientific interest due to their remarkable ability to prosper under extreme salinity. This study unveils the distinct salt-induced activation of methionine sulfoxide reductases (MsrA) from Halobacterium hubeiense, showcasing a significant enhancement in enzymatic activity across various salt concentrations ranging from 0.5 to 3.5 M. This contrasts sharply with the activity profiles of non-halophilic counterparts. Through comprehensive molecular dynamics simulations, we demonstrate that salt ions stabilize and compact the enzyme's structure, notably enhancing its substrate affinity. Mutagenesis analysis further confirms the essential role of salt bridges formed by the basic Arg168 residue in salt-induced activation. Mutating Arg168 to an acidic or neutral residue disrupts salt-induced activation, substantially reducing the enzyme activity under salt conditions. Our research provides evidence of salt-activated MsrA activity in halophiles, elucidating the molecular basis of halophilic enzyme activity in response to salts.
RESUMEN
Character strengths and training have a great impact on students' whole-person development. This study examined the applicability of the Chinese virtues questionnaire (CVQ) and the relationships between students' perceptions of virtues and resilience in Hong Kong, SAR, China. A total of 2468 pupils from primary and secondary schools in Hong Kong were recruited as the sample for this study. The results of confirmatory factor analysis (CFA) supported a measurement model of Chinese virtues, and the results of structural equation modeling (SEM) suggested that Chinese virtues were positively related to positive resilience and succumbing. Significant relationships were found between gender and students' positive resilience, and school grade level showed a significant impact on the Chinese virtues, which in turn affect resilience. Student resilience could be enhanced by nurturing virtues and related character strengths, keeping in mind the role of gender and grade level.
Asunto(s)
Pueblos del Este de Asia , Virtudes , Humanos , Hong Kong , China , Estudiantes , Encuestas y CuestionariosRESUMEN
As students' sense of school belonging (SOSB) is essential for their psychosocial well-being and academic success, developing precise measures of SOSB is critical for assessing it properly. After an unrelated item was deleted, the SOSB scale showed good psychometric properties, based on Rasch analysis of data from 36,963 students in Grade 4 or Grade 8 from four East Asian societies. While no items showed gender differential item functioning (DIF), two items showed substantial society DIF, and two items showed grade DIF in Japan and South Korea. Concurrent equating created a common measurement scale for the four societies for future comparisons. The multigroup random slope two-level analysis of the concurrent SOSB measures showed that SOSB is higher among girls than boys. From Grade 4 to Grade 8, SOSBs dropped substantially, especially for South Korea, and gender gaps decreased in Hong Kong, Japan, and South Korea. Hence, studies of interventions to improve SOSB, especially for boys and students in later grades, are vital.