RESUMEN
UNLABELLED: Traumatic brain injury (TBI) is a leading cause of disability in young people in the United States. Disorders of arousal and attention are common in closed head injury (CHI). Daytime drowsiness impairs participation in rehabilitation, whereas nighttime wakefulness leads to falls and behavioral disturbances. Sleep disturbances in TBI reported in the literature have included excessive daytime somnolence, sleep phase cycle disturbance, narcolepsy, and sleep apnea. Although well known to the clinician treating these patients, the extent and prevalence of disrupted sleep in patients in an acute inpatient rehabilitation unit has not been described. OBJECTIVE: To determine the prevalence of sleep wake cycle disturbance (SWCD) in patients with CHI in a TBI rehabilitation unit. DESIGN: Prospective observational. SETTING: Inpatient specialized brain injury rehabilitation unit. Patients. Thirty-one consecutive admissions to a brain injury rehabilitation unit with the diagnosis of CHI. RESULTS: Twenty-one patients (68%) had aberrations of nighttime sleep. There was no significant difference in Glasgow Coma Score on admission to trauma nor was there any significant difference in age between the affected and unaffected groups. Patients with SWCD had longer stays in both the trauma center (P < .003) and the rehabilitation center (P < .03). CONCLUSIONS: There is a high prevalence of SWCD in CHI patients admitted to a brain injury rehabilitation unit. Patients with SWCD have longer stays in both acute and rehabilitation settings and may be a marker for more severe injury.
Asunto(s)
Traumatismos Cerrados de la Cabeza/epidemiología , Trastornos del Sueño-Vigilia/epidemiología , Enfermedad Aguda/epidemiología , Enfermedad Aguda/rehabilitación , Adolescente , Adulto , Distribución por Edad , Comorbilidad , Trastornos de Somnolencia Excesiva/epidemiología , Femenino , Escala de Coma de Glasgow , Traumatismos Cerrados de la Cabeza/rehabilitación , Humanos , Tiempo de Internación/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Narcolepsia/epidemiología , Prevalencia , Sueño/fisiología , Síndromes de la Apnea del Sueño/epidemiología , Centros Traumatológicos/estadística & datos numéricos , Vigilia/fisiologíaRESUMEN
In Drosophila, the store-operated Ca2+ channel, TRP, is required in photoreceptor cells for a sustained response to light. Here, we show that TRP forms a complex with phospholipase C-beta (NORPA), rhodopsin (RH1), calmodulin, and the PDZ domain containing protein INAD. Proteins with PDZ domains have previously been shown to cluster ion channels in vitro. We show that in InaD mutant flies, TRP is no longer spatially restricted to its normal subcellular compartment, the rhabdomere. These results provide evidence that a PDZ domain protein is required, in vivo, for anchoring of an ion channel to a signaling complex. Furthermore, disruption of this interaction results in retinal degeneration. We propose that the TRP channel is linked to NORPA and RH1 to facilitate feedback regulation of these upstream signaling molecules.
Asunto(s)
Canales de Calcio/metabolismo , Proteínas de Drosophila , Proteínas del Ojo/metabolismo , Células Fotorreceptoras/fisiología , Secuencia de Aminoácidos , Animales , Canales de Calcio/biosíntesis , Calmodulina/metabolismo , Clonación Molecular , Drosophila melanogaster/genética , Drosophila melanogaster/fisiología , Proteínas del Ojo/biosíntesis , Proteínas del Ojo/química , Retroalimentación , Genes de Insecto , Modelos Estructurales , Mutación , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes de Fusión/biosíntesis , Transducción de Señal , Fracciones Subcelulares/metabolismo , Canales Catiónicos TRPCRESUMEN
Drosophila indirect flight muscle (IFM) contains two different types of tropomyosin: a standard 284-amino acid muscle tropomyosin, Ifm-TmI, encoded by the TmI gene, and two > 400 amino acid tropomyosins, TnH-33 and TnH-34, encoded by TmII. The two IFM-specific TnH isoforms are unique tropomyosins with a COOH-terminal extension of approximately 200 residues which is hydrophobic and rich in prolines. Previous analysis of a hypomorphic TmI mutant, Ifm(3)3, demonstrated that Ifm-TmI is necessary for proper myofibrillar assembly, but no null TmI mutant or TmII mutant which affects the TnH isoforms have been reported. In the current report, we show that four flightless mutants (Warmke et al., 1989) are alleles of TmI, and characterize a deficiency which deletes both TmI and TmII. We find that haploidy of TmI causes myofibrillar disruptions and flightless behavior, but that haploidy of TmII causes neither. Single fiber mechanics demonstrates that power output is much lower in the TmI haploid line (32% of wild-type) than in the TmII haploid line (73% of wild-type). In myofibers nearly depleted of Ifm-TmI, net power output is virtually abolished (< 1% of wild-type) despite the presence of an organized fibrillar core (approximately 20% of wild-type). The results suggest Ifm-TmI (the standard tropomyosin) plays a key role in fiber structure, power production, and flight, with reduced Ifm-TmI expression producing corresponding changes of IFM structure and function. In contrast, reduced expression of the TnH isoforms has an unexpectedly mild effect on IFM structure and function.
Asunto(s)
Proteínas de Drosophila , Drosophila/fisiología , Vuelo Animal/fisiología , Miofibrillas/fisiología , Tropomiosina/fisiología , Alelos , Animales , Clonación Molecular , Drosophila/embriología , Drosophila/genética , Femenino , Genes de Insecto/genética , Haploidia , Músculo Esquelético/química , Músculo Esquelético/fisiología , Músculo Esquelético/ultraestructura , Mutación , Miofibrillas/ultraestructura , Tropomiosina/genética , Tropomiosina/aislamiento & purificaciónRESUMEN
Using overlapping synthetic deficiencies, we find that a haplo-insufficient locus affecting flight behavior and the myosin light chain-2 gene co-map to the Drosophila melanogaster polytene chromosome interval 99D9-E1 to 99E2-3. From screening over 9000 EMS-treated chromosomes, we obtained alleles of two complementation groups that map to this same interval. One of these complementation groups lfm(3)99Eb, exhibits dominant flightless behavior; thus, flightless behavior of the deficiency is in all likelihood due to hemizygosity of this single locus. Rescue of flightless behavior by a duplication indicates that the single allele, E38, of the Ifm(3)99Eb complementation group is a hypomorph. Based upon its map position and a reduction in concentration of myosin light chain-2 mRNA in heterozygotes, we propose that Ifm(3)Eb(E38) is a mutant allele of the myosin light chain-2 gene. Our genetic analysis also resulted in the identification of four dominant flightless alleles of an unlinked locus, l(3)nc99Eb, that exhibits dominant lethal synergism with Ifm(3)99Eb.