RESUMEN
Hormonal regulation of receptor expression and glycogen concentrations in the epithelial cells of the oral cavity remains poorly studied. Adhesion of microorganisms to the vaginal epithelium correlates with their adhesion to the buccal epithelium. Analysis of the correlation between Candida adhesion to the vaginal and buccal epithelium depending on the hormonal status has demonstrated that activity of Candida albicans strains in the buccal epithelium correlates with their adhesion activity in the vaginal epithelium, with the coefficient of correlations (r) reaching 0.76.
Asunto(s)
Candida albicans/fisiología , Adhesión Celular/fisiología , Células Epiteliales/microbiología , Mucosa Bucal/microbiología , Vagina/citología , Células Cultivadas , Estrógenos/metabolismo , Femenino , Humanos , Ciclo Menstrual/fisiologíaRESUMEN
Experiments with passive transfer of immunocompetent cell and serum demonstrated suppression of tumor growth after transfer of splenocytes from animals immunized with Trypanosoma cruzi. The nonspecific constituent of antitumor effect of immunocompetent cells was detected. The previously reported oncoprotective effect of mucins 2 and 3 was not confirmed.
Asunto(s)
Anticuerpos Antiprotozoarios/inmunología , Carcinoma de Ehrlich/terapia , Trasplante de Células , Bazo/citología , Trypanosoma cruzi/inmunología , Animales , Carcinoma de Ehrlich/inmunología , Carcinoma de Ehrlich/patología , Femenino , Inmunización , Ratones , Ratones Endogámicos BALB C , Mucinas/inmunología , Trasplante de Neoplasias , Proteínas Protozoarias/inmunología , Bazo/inmunología , Carga TumoralRESUMEN
Adhesion activity of L. acidophilus NK1, L. fermentum 90 TS4, and C. albicans 506 B on female vaginal epithelium was studied. Adhesion of various lactobacillus species was hormone-dependent. Adhesion of C. albicans 506 B was not associated with estrogen level. The effects of synthetic drugs and phytopreparations used for hormone replacement on adhesion of vaginal microbiocenosis members varied.
Asunto(s)
Lactobacillus/fisiología , Vagina/microbiología , Adolescente , Adulto , Adhesión Bacteriana/efectos de los fármacos , Candida albicans/efectos de los fármacos , Candida albicans/fisiología , Combinación de Medicamentos , Epitelio/microbiología , Estradiol/análogos & derivados , Estradiol/farmacología , Femenino , Humanos , Lactobacillus/efectos de los fármacos , Levonorgestrel/farmacología , Persona de Mediana Edad , Fitoestrógenos/metabolismo , Fitoestrógenos/farmacología , Vagina/metabolismo , Adulto JovenRESUMEN
Phytoestrogens present in the plants endemic for Chile were studied. The effects of phytoextract [specimen of preparation No. 181 (fraction b)] on target tissues were similar to those of estradiol. The preparation inhibited the stimulatory effect of estradiol on vaginal lactobacilli population.
Asunto(s)
Fitoestrógenos/farmacología , Extractos Vegetales/farmacología , Vagina/microbiología , Animales , Carga Bacteriana , Recuento de Células , Cimicifuga/química , Método Doble Ciego , Eosinófilos/citología , Estradiol/farmacología , Femenino , Lactobacillus/efectos de los fármacos , Metagenoma , Células Musculares/citología , Ovariectomía , Ratas , Ratas Sprague-Dawley , Útero/citología , Vagina/efectos de los fármacosRESUMEN
The lectin-binding component added to gel was studied in vivo as a potential therapeutic and preventive agent in experimental vaginal candidiasis. Laboratory studies demonstrated its antimycotic effect in relapsing vaginal candidiasis in mice.
Asunto(s)
Proteínas Bacterianas/farmacología , Candida albicans/fisiología , Candidiasis/tratamiento farmacológico , Vaginitis/tratamiento farmacológico , Animales , Proteínas Bacterianas/uso terapéutico , Candida albicans/efectos de los fármacos , Candidiasis/microbiología , Femenino , Geles/farmacología , Geles/uso terapéutico , Interacciones Huésped-Patógeno , Ratones , Membrana Mucosa/microbiología , Vaginitis/microbiologíaRESUMEN
Changes in the cell wall of yeast-like fungi Candida albicans caused by bacterial enzyme lyticase were studied under an electron microscope. The results were compared with the findings on phagocytosis of lyticase-treated Candida albicans by rat alveolar macrophages. It was shown that destruction of the mannan and fibrous outer layers of the cell wall of yeast-like fungi treated with the enzyme led to the release of intracellular organelles and their content. Lyticase treatment reduced the resistance of Candida albicans to intracellular digestion by macrophages contributing to completion of phagocytosis.
Asunto(s)
Candida albicans/efectos de los fármacos , Glucano Endo-1,3-beta-D-Glucosidasa/metabolismo , Macrófagos Alveolares/microbiología , Complejos Multienzimáticos/metabolismo , Péptido Hidrolasas/metabolismo , Animales , Candida albicans/ultraestructura , Hongos/efectos de los fármacos , Hongos/ultraestructura , Microscopía Electrónica de Transmisión , Fagocitosis , RatasRESUMEN
Lyticase (a bacterial enzyme) was tested as a new antimycotic drug. Of all objects studied, Cellulomonas cellulans AC-870 strain proved to be most productive for this enzyme. A technology for lyticase isolation and purification was proposed. An experimental model of recurrent vaginal candidiasis was created. The model includes combined antibiotic and estradiol therapy. Antimycotic effect of lyticase on the model of recurrent vaginal candidiasis in mice was demonstrated.
Asunto(s)
Candidiasis Vulvovaginal/tratamiento farmacológico , Glucano Endo-1,3-beta-D-Glucosidasa/metabolismo , Complejos Multienzimáticos/metabolismo , Péptido Hidrolasas/metabolismo , Animales , Femenino , Glucano Endo-1,3-beta-D-Glucosidasa/uso terapéutico , Ratones , Complejos Multienzimáticos/uso terapéutico , Péptido Hidrolasas/uso terapéuticoRESUMEN
Laboratory studies of lyticase (enzymatic drug) as an antimycotic agent were carried out. The enzyme reduced optical density of Candida albicans test culture, inhibited adhesion of yeast-like fungi on vaginal epitheliocytes, stimulated the formation of germinative tubes, and made Candida albicans more available for phagocytosis.
Asunto(s)
Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Glucano Endo-1,3-beta-D-Glucosidasa/farmacología , Complejos Multienzimáticos/farmacología , Péptido Hidrolasas/farmacología , Animales , Antifúngicos/uso terapéutico , Candida albicans/fisiología , Candidiasis/tratamiento farmacológico , Células Cultivadas , Células Epiteliales/microbiología , Femenino , Glucano Endo-1,3-beta-D-Glucosidasa/uso terapéutico , Humanos , Ratones , Complejos Multienzimáticos/uso terapéutico , Péptido Hidrolasas/uso terapéutico , Fagocitosis , Vagina/citologíaRESUMEN
The experiments with passive transfer of splenocytes obtained from animals immunized with Trypanosoma cruzi lysate revealed the role of cell-mediated component of the immunity in the antitumor effect of T. cruzi. The common features of T. cruzi antigens and tumor-specific antigens of Ehrlich's adenocarcinoma were demonstrated. These antigens were shown to have common epitopes with mammalian mucins. The oncoprotective effect was achieved by immunization with type II and III mucins and was reproduced after passive transfer of splenocytes from immunized animals.
Asunto(s)
Anticuerpos Antiprotozoarios/inmunología , Antineoplásicos/farmacología , Carcinoma de Ehrlich/prevención & control , Trypanosoma cruzi/inmunología , Animales , Ratones , Ratones Endogámicos BALB C , Mucina 2/inmunología , Mucina 3/inmunología , Trasplante de NeoplasiasRESUMEN
A sampling of lactobacilli from the German National Collection of Microorganisms and L. fermentum 90 TS-4 (21) reference strain clone 3 (Russian Federation) were studied. The results indicate that the receptors on the surface of lactobacillus strains from the German collection had no structures complementary to type 1 fimbriae, though adhesins of some of them reacted with mannose and galactose receptors. Adhesion on a monolayer of continuous cell cultures showed that adhesion activity of lactobacilli was a function of many derivatives, and hence, the choice of a model for evaluation of the adhesion characteristics of the strain should be based on adhesins exhibiting universal properties in different test systems. One of them can be lectin-binding adhesin; its expression on the surface of cultures of lactobacilli from the German collection varies within the same range as was shown previously for lactobacilli, studied by the same criterion. The molecular weight of lectin-binding adhesin is 25-30 kDa, and the corresponding receptors are frequently present on various eukaryotic cells, and hence, cell models can be considered as the most adequate for studies of the competitive interactions between lactobacilli and adhesins of pathogenic microorganisms.
Asunto(s)
Adhesinas Bacterianas/metabolismo , Adhesión Bacteriana/fisiología , Lactobacillus/metabolismo , Probióticos , Adhesinas Bacterianas/química , Fimbrias Bacterianas/metabolismo , HumanosRESUMEN
Trypanosoma cruzi antibodies that are important in the anticancer effect of this species of Trypanosoma were first detected in 14% of the 374 examined intact mice. These background antibodies were polyclonal, i.e. they reacted with one or other of 9 genetically different T. cruzi clones and detected antigenic determinants in different structures of a Trypanosoma cell. Their spread in the population varied with animal batches; the titers were not greater than 1:40 and the level remained steady during a one-month observation. The growth of Ehrlich's adenomacarcinoma inoculated to these mice was inhibited by 1.5-2.5 times and, in some cases, terminated in total regression. The paper discusses the capacities of such antibodies in man and their inductor-commonness of the antigens of the normal intestinal microflora and the cells of the vertebrate with T. cruzi.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Antineoplásicos/sangre , Carcinoma de Ehrlich/inmunología , Trypanosoma cruzi/inmunología , Animales , Anticuerpos Antiprotozoarios/inmunología , Especificidad de Anticuerpos , Antígenos de Protozoos/inmunología , Antineoplásicos/inmunología , Carcinoma de Ehrlich/diagnóstico , Progresión de la Enfermedad , Ratones , Factores de TiempoRESUMEN
Antagonistic activities of L. fermentum strain 90 TS-4 (21), L. casei ATCC 27216, and L. acidophilus ATCC 4356 and bactericidal activity of lactobacillus culture fluid towards E. coli strain K12, S. aureus, and S. epidermidis test cultures were studied. The bactericidal effect of L. fermentum strain 90 TS-4 (21) clone 3 culture fluid preparation (pH 6.0) on the test cultures was dose-dependent. Adhesion of C. albicans yeast-like fungi to vaginal epitheliocytes was more pronounced for strains isolated from women with asymptomatic infection than for strains isolated from women with manifest forms. L. fermentum strain 90 TS-4 (21) clone 3 culture fluid preparation modulated adhesion of yeast-like fungi only if the fungal strain was initially highly adherent.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Candida albicans/efectos de los fármacos , Limosilactobacillus fermentum/fisiología , Vagina/efectos de los fármacos , Vagina/microbiología , Candida albicans/patogenicidad , Candida albicans/fisiología , Candidiasis Vulvovaginal/microbiología , Adhesión Celular/efectos de los fármacos , Células Epiteliales/efectos de los fármacos , Células Epiteliales/microbiología , Escherichia coli K12/efectos de los fármacos , Femenino , Humanos , Técnicas In Vitro , Lactobacillus acidophilus/fisiología , Lacticaseibacillus casei/fisiología , Staphylococcus aureus/efectos de los fármacos , Staphylococcus epidermidis/efectos de los fármacosRESUMEN
The content of Tamm-Horsfall protein was measured in the urine of humans without renal diseases, pregnant women, and donors and recipients of renal transplant using a new test system for measuring Tamm-Horsfall protein including antigenic diagnostic agent and immune serum. Production of antibodies to Tamm-Horsfall protein was characterized using antigen diagnostic system.
Asunto(s)
Inmunoglobulina A/sangre , Trasplante de Riñón/inmunología , Mucoproteínas/inmunología , Mucoproteínas/orina , Donantes de Tejidos , Trasplante , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Embarazo , Factores de Tiempo , UromodulinaRESUMEN
Lyzed epimastigotes of Trypanosoma cruzi clones P209-1, Gamba1, Sp104-1, MASu, Y7/1, MN12, Cl-Brener, 86/2036, Y7/2-1 inhibit the growth of Ehrlich adenocarcinoma in mice. the tumor decreased 1.5-3 times after 12 daily injections of lysates from 15 million epimastigotes. The protective effect progressed after the injections were discontinued and depended on the dose and lysate producer clone. Trypanosoma lysates in the studied doses were nontoxic.
Asunto(s)
Adenocarcinoma/tratamiento farmacológico , Antineoplásicos/aislamiento & purificación , Antineoplásicos/uso terapéutico , Carcinoma de Ehrlich/tratamiento farmacológico , Extractos Celulares/uso terapéutico , Trypanosoma cruzi/genética , Adenocarcinoma/patología , Animales , Carcinoma de Ehrlich/patología , RatonesRESUMEN
Six lactobacillus species and 4 clones of one of them were studied in order to clear out the ratio between the adhesion capacities of concanavalin A-reactive glycoprotein adhesins on the surface of the bacterial cell and glycoprotein adhesins released into the broth during culturing in liquid nutrient media. The adhesive activity of cultures is largely determined by the strain rather than species appurtenance. Elimination of glycoprotein adhesins from the bacterial cell and their antagonistic activity towards Candida albicans were demonstrated in specific interactions of glycoprotein adhesins with immune serum and concanavalin A.
Asunto(s)
Adhesinas Bacterianas/metabolismo , Adhesión Bacteriana , Lactobacillus/química , Adhesinas Bacterianas/análisis , Aglutinación/efectos de los fármacos , Adhesión Bacteriana/efectos de los fármacos , Técnicas Bacteriológicas , Candida albicans/efectos de los fármacos , Concanavalina A/farmacología , Medios de Cultivo/química , Células Endoteliales/efectos de los fármacos , Femenino , Humanos , Lactobacillus/efectos de los fármacos , Lactobacillus/crecimiento & desarrollo , ProbióticosRESUMEN
We examined production of the protein-lipoteichoic complex detectable by concanavalin A in six lactobacillus strains. A correlation was found between detection of this complex by both reaction with concanavalin A and agglutination with strain-specific antiserum. The cultures were characterized by expression of different types of adhesins. Among them, strains were differentiated with low adhesion activity and intensive production of the protein-lipoteichoic complex and strains with wide adhesin spectrum not producing the complex. We assume that a combination of lactobacillus variants differing in production of the protein-lipoteichoic complex and adhesion potency can be a basis of efficient probiotic preparation.
Asunto(s)
Adhesinas Bacterianas/aislamiento & purificación , Lactobacillus/química , Probióticos/química , Pruebas de Aglutinación , Adhesión Bacteriana/fisiología , Concanavalina A , Complejos Multiproteicos/metabolismo , Receptores de Superficie Celular/metabolismo , Especificidad de la EspecieRESUMEN
The evidence has been produced that immunological mechanisms are involved in the known anticancer phenomenon of T. cruzi. Non-inbred albino mice were immunized with avirulent cultures of three strains and seven clones and then transplanted a tumor--sarcoma-180 or Ehrlich's adenocarcinoma. The used cultures induced the generation of T. cruzi antibodies whose level peaked by postimmunization days 50-60: the titers being 1:40-1:80 and the spread among the mice being 60%. Concurrently, immunization against T. cruzi provided a certain oncoprotective effect. In the immunized mice, the sizes of sarcoma-180 and adenocarcinoma were 1.5-2.0 and 2.0-2.5 times, respectively, less than those in the non-immunized ones. The antitumor protection was directly related to the murine blood T. cruzi antibody level during which implantation of a tumor occurred. At the peak of an immune response, the effect was 2 times higher than that in the early postimmunization periods. T. cruzi strains that were more immunogenic than clones ensured a more significant oncoprotection. The latter was more considerable in mice having antibody titers of 1:40-1:80 than in those with antibody titers of 1:10-1:20 and particularly in the animals showing no humoral response to the administration of the parasites at all.
Asunto(s)
Adenocarcinoma/prevención & control , Enfermedad de Chagas/inmunología , Inmunización , Vacunas Antiprotozoos/administración & dosificación , Sarcoma Experimental/prevención & control , Neoplasias Cutáneas/prevención & control , Trypanosoma cruzi/inmunología , Animales , Anticuerpos Antiprotozoarios/sangre , Inyecciones Intramusculares , Inyecciones Intraperitoneales , Inyecciones Subcutáneas , Ratones , Especificidad de la Especie , Factores de Tiempo , Vacunas Atenuadas/administración & dosificaciónRESUMEN
A new test system for measuring Tamm-Horsfall protein was developed. The system consists of antigenic diagnostic agent and immune serum. The specificity and sensitivity of the reagents were determined. The system was tested on 52 urine samples from subjects without renal diseases.
Asunto(s)
Mucoproteínas/orina , Animales , Eritrocitos/inmunología , Humanos , Sueros Inmunes , Inmunodifusión/métodos , Masculino , Mucoproteínas/inmunología , Pruebas de Neutralización/métodos , Conejos , Sensibilidad y Especificidad , Ovinos , UromodulinaRESUMEN
Phagocytosis of polymer microspheres both modified and unmodified by bioligands (gelatin, fibronectin and A protein) by leukocytic blood cells of patients with acute pancreatitis. The presence of bioligands in the surface of polymer microspheres and their activity were monitored by the reaction of latex agglutination with an appropriate ligand being involved. The study resulted in designing a method of phagocytosis implementation that can be used to evaluate the content of certain receptors in cellular membranes and, subsequently, to define the changed receptor cell apparatus caused by a disease. The nature of polymer microspheric bioligands immobilized to the surface was shown to influence significantly the activity of leukocytic blood cells.
Asunto(s)
Neutrófilos/fisiología , Pancreatitis/sangre , Fagocitosis/fisiología , Receptores de Superficie Celular/metabolismo , Enfermedad Aguda , Fibronectinas/metabolismo , Gelatina/metabolismo , Humanos , Técnicas In Vitro , Ligandos , Microesferas , Neutrófilos/metabolismo , Receptores de Fibronectina/metabolismo , Proteína Estafilocócica A/metabolismoRESUMEN
Adhesive activity of Candida albicans towards vaginal epitheliocytes is hormone-dependent. Two types of C. albicans adhesins sensitive to polyphenyloxidase and asparaginase were detected. Laser irradiation nonspecifically modulated both adhesin types. Population relationships between fungi and lactobacilli in patients with vaginal candidal infection and in C. albicans carriers were studied. Genodiagnostic method for identification of C. albicans and morphogenesis-associated genes of these yeast-like fungi was approved.