RESUMEN
The use of various types of stimulation of fish spermatozoa in order to increase the survival of defrosted cells is a promising direction in the field of low-temperature preservation. In this work, the use of acoustic-mechanical effects on the sperm of sturgeons (in particular, sterlet) was studied for the first time. The technical characteristics of the assembled experimental device for acoustic-mechanical effects on fish sperm are given. As a result of this work, the optimal parameters of exposure to increase the preservation of defrosted germ cells were selected.
Asunto(s)
Criopreservación , Semen , Acústica , Animales , Peces , Masculino , Motilidad Espermática/fisiología , Espermatozoides/fisiologíaRESUMEN
The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and Leuconostoc spp. in different ratios) and different types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.