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The examinations of rats hippocampus were made after ischaemia (the rats vertebral and common carotid arteries were closed for 20 minutes). Serial sections of the brains were stained with hematoxylin and eosin. 24 hours after completing the experiment a small number of cells in the field CA1 was damaged. They were dark and shrunken. 120 hours after the experiment strong damages affected large groups of pyramidal cells, especially in field CA1. The changes were asymmetrical and regarded one hemisphere of the brain only.

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Odontoblasts located in the peripheral layer of the pulp reflect current state of the process connected with the formation of the secondary dentin and thereby they protect teeth from inflammatory processes (1, 3, 4, 6, 10). Odontoblasts are highly specialized cells that can react to the mechanical, thermic and toxic and bacterial traumas (12, 13, 14, 15).

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Examinations of the influence of 6-OH-DA (6-hydroxydopamine) upon the structure of nerve cells and their axons have been conducted by many authors and in various aspects (1, 2, 8, 10). They have concerned histochemistry, fluorescence and ultrastructure of neurons and have shown that the substance evokes various changes in the perikaryons and axons of adrenergic neurons. The wall of the small intestine is innervated by external autonomyous nerves and fibres of the intramural autonomyous plexuses. External autonomyous fibres make numerous anastomoses with the neurons of the two intramural plexuses and ganglia: the myenteric one (Auerbach's) and the submucosal one (Meissner's) (6). Both ganglia run along the length of the intestine and their neurons apart from making anastomoses, send axons to the nonstrained muscles cells and glandular cells in the mucosa. As a consequence, the active phase is easily carried over along the alimentary system stimulating action of nonstrained muscles and glandular cells. 6-OH-DA exerts strong action upon the nerve cells and ends, that is why we have decided to examine the changes taking place in the ileum when the substance starts to operate.

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Kidneys of the rats kept on a diet containing electroenergetical ashes were examined histochemically. An increase in activity of enzymes in cells of kidney canaliculi in animals which were the longest subject to experiment, were observed.

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Normal metabolism, regulation of stimulation and checking of electrolyte secretion in the gastric juice depend on the way of feeding and its nutritional ingredients. Feeding experimental animals with fodder containing power plant ashes or loess dusts causes a change in the gastric juice reaction, thus resulting in a different assimilation of elements contained in the fodder. There was shown an increase of some elements content (Mg, Na, Cu, Fe), a decrease in the content of some others (Pb,Zn) in the gastric juice and a positive tendency for blocking by calcium ions of heavy metals contained in the fodder by the mucous membrane of the stomach.

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Mature male rats received encortone in a dose of 1 mg/kg of body mass for 7 days and 30 days. The following changes were observed in the cells of parotid gland of the experimental animals in comparison with the control: a) after 7 days of encortone treatment increase of secretory cells activity appeared in the intensive production and secretion, b) after 30 days of encortone treatment a decrease in secretion production. Moreover, hyperplasia of serous cells, hyperthropy of cell nuclei, pycnosis of nuclei in some serous cells and hyperplasia of epithelium in some intercalated ducts were observed.

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The examination studied behaviour of rats fed on natritive material containing soil dust or electroenergetic ashes. Food containing the dust or ashes was administered to animals in the amount of 30 g a day for 26 weeks. Body weight was controlled in all animals and their motor activity, motor coordination and cognitive activity were examined. It has been settled that rats fed an food with the soil dust supplement showed higher motor activity and acquired greater motor coordination than animals fed either on food with the electroenergetic ashes supplement or on conventional food. During the first weeks of experiment, cognitive activity was getting lower in animals in both experimental groups and control group, and then only in the group of animals fed on food with the soil dust supplement.

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The influence of Encortone treatment in the dose of 1 mg/kg of body mass on the secretory segments of male rat parotid gland was investigated. The changes of section area of cell nuclei, secretory cells, serous acini and changes of the number of cells in the acini were statistically analyzed. The observed changes depended upon the period of Encortone treatment. Seven-day Encortone treatment caused a statistically significant increase of the cell nuclei size and decrease of the cell and acini sizes. Changes of these parameters were result of cell hyperplasia and formation of new acini. Thirty-day Encortone treatment caused hypertrophy of cell nuclei, cells and acini of rat parotid.

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The experiment was carried out on white Wistar rats. The rats in the experimental groups received fodder containing power station ashes for various periods of time (from 6 to 18 weeks). During the experiment, the body weight of rats was recorded, the amount of trace elements in the liver was estimated, and morphological tests of the liver were carried out. The results of these experiments indicate that feeding the rats on fodder containing power station ashes causes several percent increase in the body weight and slight differences in accumulation of trace elements. Morphological changes in the liver are connected with the vacuolization of the hepatocytes' cytoplasma and an increase in the amount of phagocytic cells in hepatic sinuses. The intensity of morphological changes was correlated with the time of experiment.

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Investigations refer to the influence of fodder containing soil dust as one of the ingredients on morphology of the liver and the weight of experimental animals. Fodder containing soil dust was given to animals for various periods of time, the longest was 18 weeks. The results indicate that the intensity of morphological changes in the liver depends on the time of giving fodder. The observed changes referred to the vacuolization of hepatocytes, increase in the number of stellate cells Browicz-Kupffer, and apart from that lymphocytic infiltrations occurred locally. The weight of the experimental animals in the initial period (up to 3 weeks) decreased and then came back to normal in the later period, but in some animals it even decreased.

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Fetal liver was examined in rats, whose mothers during pregnancy received Turinal (firm: Chemical Works of Gedeon Richter LTD Budapest, Hungary) in a dosage of 0.5 mg/kg BW in the experimental group I and 1 mg/kg BW in the experimental group II. Reaction to the activity of succinate dehydrogenase, lactic dehydrogenase, acid phosphatase, glucose-6-phosphatase, PAS-reaction (Periodic acid Schiff reaction) and hematoxylin-eosin staining were carried out. Changes in the activity of examined enzymes and PAS-reaction in fetal liver depending on dosage were observed. These changes testify to a large influence of administered hormone on metabolism of developing fetal liver.

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The studies were performed on 44 white female Wistar rats which were intratracheally administered the suspension of the soil dust and the electro-energetic ashes. The electro-energetic ashes were collected from 6 different local heat and power generating plants while the soil dust from several random places of our country. The statistical analysis of the body and the lung mass of the animals subjected to the single dust and ash insufflation was performed. The applied variants proved the statistically significant differences between the body and the lung mass. The observed differences are connected with the kinds of dust and ash used in the experiment.

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The studies of the effect of the soil dust on the cornea and the conjunctiva of the experimental animals have been performed. The rats were divided into 3 experimental groups and 1 control group. The animals were given 3 different, selected at random, kinds of soil dust to the conjunctival sac 3 times daily for 7 days. The results show that the soil dust has the negative effect on the organ of sight. The cornea is particularly sensitive to the action of dust, which evidenced itself by the significant changes in the histochemical reactions.

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There was examined pulmonary tissue of white rats, which had been administered intratrachealy a single dose of the respirable fraction of ashes sample from 6 different power stations elektrohasting plants and hasting plants in Poland (0.2 ml suspension; 50 mg of the examined sample in 0.6 cm3 of NaCl solution). 9 months after the application of the ashes, biopsies of the left lung were taken and there was determined the activity of acid phosphatase (AcP) and adenosinetriphosphatase (ATP-ase) histoenzymatically. There was found sensitivity of these hydrolases and changes of their activity connected with chemical composition of the examined ashes.

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There was examined the biological activity of soil dusts using histochemical methods. Intratracheal administration of dusts used in this study is a common method for testing activity of industrial dusts. The used soil dusts were characterized by high content of free silicon dioxide, 3 times higher than its content in dusts from power stations released in the process of burning coal and approximately to the amount in graphite dust. Both coal dusts and graphite dusts absorbed by the lungs cause silicoanthracosis. The investigations have been shown that soil dusts caused stimulation of the mitochondrial metabolism giving an increase of the activity of succinic dehydrogenase, lactic dehydrogenase, and ATPase induced by Mg++ ions. This was additionally confirmed by an increase of NADP activity which is an enzyme binding a chain or reactions regulating the hydrocarbonic metabolism. There was also observed an increased activity of the hydrolytic enzyme acid phosphatase. High activity occurred in the epithelium of bronchi and bronchioli and focally in pulmonary parenchyma.

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For seven days, three times a day, female rats were instilled into their conjunctival sacs suspensions of power plant dusts, soil and clay. Clinically observed were inflammatory changes of the conjunctiva and cyclids margins as well as traumatisation of the corneal and conjunctival epithelia, appearing at different stages on the experiment. Cytoenzymatic examinations indicated disturbances of oxidation and reduction processes, changes in dialysis through physiologic membranes and dissociation of protein synthesis.

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The experiments were carried out in Wistar rats which were given intratracheally samples of carbon ashes or soil dust in the form of the respirable fraction. The morphologic changes and the glycogen content in the experimental animals' livers were evaluated. The content of the trace elements was determined in the applied carbon ashes and soil dust by means of the X-rays diffraction method. The results of the studies show that the morphologic changes in liver depend on the kind of dust and are related to the content of the trace elements and the free silicon dioxide in the carbon ashes and the soil dust.

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