RESUMEN
Specific features of the automated computerized diagnostic complex based on electrometric methods and used for the auricular diagnostics have been considered. A functional scheme of the complex is given. Biophysical aspects of measuring the skin conductivity in acupuncture zones of the auricle are discussed. Special attention is paid to the diagnostic algorithms and to the control for their efficiency.
Asunto(s)
Puntos de Acupuntura , Diagnóstico por Computador , Oído Externo , Tamizaje Masivo/métodos , Algoritmos , Conductividad Eléctrica , Humanos , Sensibilidad y EspecificidadAsunto(s)
Fisura del Paladar/terapia , Levamisol/uso terapéutico , Adolescente , Niño , Preescolar , Fisura del Paladar/sangre , Fisura del Paladar/inmunología , Terapia Combinada , Femenino , Hemostasis/efectos de los fármacos , Humanos , Inmunidad/efectos de los fármacos , Masculino , Periodo PosoperatorioRESUMEN
The distribution pattern of marker enzymes (Na, K-ATPase, acetylcholinesterase) in three fractions of synaptic membranes (SM) of rat brain were studied. The effects of three anticonvulsive agents on Na, K-ATPase from the total fraction of rat brain SM and purified membrane preparation from ox brain were estimated by different methods. Under optimal conditions (Na/K = 5) diphenylhydantoin (DPH) at a concentration of 0,1 mM activates Na, K-ATPase from the total SM fraction only in the absence of ouabain, whereas carbamazepine and pyrroxane taken at the same concentrations have no effect on Na, K-ATPase, irrespective of the type of the enzyme assay. DPH seems to compete with ouabain. Under non-optimal ionic conditions (Na/K = 250) all the anticonvulsive substances studied inhibit Na, K-ATPase of the total SM fraction. The mixture of hydrophobic agents (propylene glycol and ethanol) used to dissolve carbamazepine inhibits Na, K-ATPase from the total SM fraction only under non-optimal conditions. The inhibiting effect of the anticonvulsive substances under study on Na, K-ATPase from the purified membrane preparations is maximal at the concentration of 10(-6) M; at higher concentrations the effect is less pronounced.