RESUMEN

Modified proteins were determined by isoelectric focusing in borate-polyol system with subsequent colorimetry (micromethod) and electrophoresis of blood serum on paper with subsequent TCA-ethanol treatment. Increased levels of glycated hemoglobin and modified albumin and changed light absorbance of glycated albumin were detected. The levels of glycated hemoglobin assessed by the micromethod and colorimetry without calibration did not correlate.

Asunto(s)

Proteínas Sanguíneas/análisis , Diabetes Mellitus/sangre , Electroforesis de las Proteínas Sanguíneas/métodos , Electroforesis de las Proteínas Sanguíneas/estadística & datos numéricos , Colorimetría/métodos , Electroforesis en Papel/métodos , Humanos , Focalización Isoeléctrica/métodos