RESUMEN

We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(L-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(L-lysine)-DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental results indicate high sensitivity and selectivity of the immunosensor.

Asunto(s)

Anticuerpos Antinucleares/sangre , Técnicas Biosensibles/métodos , Cuarzo/química , Animales , Anticuerpos/química , Anticuerpos/inmunología , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/inmunología , Asma/inmunología , Bovinos , ADN/química , ADN/inmunología , ADN de Cadena Simple/inmunología , Desoxirribonucleasas/química , Oro/química , Humanos , Inmunoensayo/métodos , Inmunoglobulina G/inmunología , Lupus Eritematoso Sistémico/inmunología , Microscopía de Fuerza Atómica , Polilisina/química , Reproducibilidad de los Resultados , Albúmina Sérica Bovina/química