RESUMEN
Tick-borne encephalitis virus strain 205 was characterized by such sign as the presence of negative colonies. In the swine fibroblast cell line the population of the strain was represented mainly by large plaque (67.3%). Medium-sized plaques prevailed in chick embryo fibroblast cell culture. Protamine sulfate and magnesium chloride reduced the period of incubation, increased the resolving capacity of the plaque method and the proportion of medium- and small-sized plaques. Agarose B rendered the virus colonies less contrasting. The production strain and the clones of its population were found to have pronounced reproductive and protective properties. The S+ variants of the virus exhibited the highest reproductiveness.
Asunto(s)
Virus de la Encefalitis Transmitidos por Garrapatas/crecimiento & desarrollo , Vacunas Virales , Animales , Virus de la Encefalitis Transmitidos por Garrapatas/efectos de los fármacos , Virus de la Encefalitis Transmitidos por Garrapatas/inmunología , Cloruro de Magnesio/farmacología , Ratones , Ratones Endogámicos BALB C , Protaminas/farmacología , Pase Seriado , Vacunas de Productos Inactivados , Ensayo de Placa Viral , Cultivo de VirusRESUMEN
The characterization of such properties of tick-borne encephalitis virus strain 205 and its clones as virulence, thermal stability and sensitivity to 2.5 M urea was obtained. The following combination of the genetic markers of strain 205 was established: mNic+, mNsc+, T50+, Ur. The clones possessed pronounced virulence, but showed different heat sensitivity (T50+, T50 +/-) and resistance to the action of urea (Ur and U +/-). At the early terms of observation, the clone isolated from a large viral plaque was more antigen-active.