RESUMEN
We have shown recently that the first stage of the common site for the aromatic pathway in Pseudomonas aeruginosa, P. putida, P. fluorescens, P. acidovorans, and P. testosteroni is controlled by repression of 3-deoxy-D-arabinoheptulosenate-7-phosphate-synthase synthesis with phenylalanine and tyrosine. This work is devoted to the study of genetic control of the remaining six stages of the common site for aromatic pathway in representatives of Pseudomonas genus using auxotrophic and regulatory mutants it was shown that genes aroB, aroD, aroE, aroL, aroA, and aroC operate constitutively in P. putida, P. mendocina, P. marginata, and P. acidovorans.
Asunto(s)
3-Desoxi-7-Fosfoheptulonato Sintasa/genética , Genes Bacterianos , Pseudomonas/genética , 3-Desoxi-7-Fosfoheptulonato Sintasa/biosíntesis , Mutación , Fenilalanina/fisiología , Pseudomonas/enzimología , Proteínas Represoras/fisiología , Tirosina/fisiologíaRESUMEN
The trpBA genes of Pseudomonas mendocina and P. marginata were cloned in Escherichia coli using pBR322 as a vector. Transcription of the cloned genes took place from their own promoters, in the following order: trpB-->trpA. The latter genes were not linked to the trpF gene and could not be induced by indole glycerol phosphate. Thus, the trpBA cluster of P. mendocina and P. marginata are distinguished from that of P. putida, P. aeruginosa (trpIBA) and P. acidovorans (trpFBA).