RESUMEN
Contamination of Japanese quail, strain Pharaoh, cell culture with oncogenous and infectious avian viruses was studied. The susceptibility of the embryonal cell cultures of the Japanese quail, strain Pharaoh, to measles, parotitis and fixed rabies viruses was also determined. It was found that the sera of pubertal quails had no antibody to Rous sarcoma virus (RSV), strains Brian, RSV (RAV-1), Schmidt-Ruppin, Carr-Zilber, as well as to Marek's disease and Newcastle disease viruses. No reverse transcriptase activity was detected in the embryonal alantoic fluid of this avian species. The quails were less susceptible, as compared to the chicken, to Schmidt-Ruppin and Carr-Zilber strains of RSV. Measles, parotitis and fixed rabies viruses reproduced actively in the Japanese quail, Pharaoh strain, embryonal cell cultures. It is suggested that the embryonal cell cultures of this avian species can be used as a leukemia-free substrate for experimental studies and manufacturing of viral vaccines.
Asunto(s)
Alpharetrovirus/aislamiento & purificación , Cultivo de Virus , Animales , Virus del Sarcoma Aviar/patogenicidad , Células Cultivadas , Pollos , Coturnix/embriología , Coturnix/microbiología , Fibroblastos/microbiología , Virus del Sarampión/crecimiento & desarrollo , Virus de la Parotiditis/crecimiento & desarrollo , Virus de la Rabia/crecimiento & desarrollo , Especificidad de la EspecieRESUMEN
The results of immunological study of oncornavirus (LPV strain) produced by a continuous human T-9 cell line are presented. The method of immunodiffusion in agar revealed the following constituent antigens in the virus: (a) those identical to internal and external antigens of Mason-Pfizer oncornaviruses particles; (b) those specific only for LPV virus and not common in other known oncornaviruses of mammals. These antigens were common in LPV virus from T-9 culture and from xenogeneic T-BHK culture in which Mason-Pfizer virus antigens were absent completely; and (c) interspecific antigen of oncornavirus C type present in minimal amounts. Localization of the above antigens in virus particles of LPV oncornavirus was confirmed by the electron immunoenzyme method. Upon separation of virus polypeptides in polyacrylamide gel it was found that the antigens cross-reacting with Mason-Pfizer virus as well as LPV specific antigens were localized both in the main inner virus protein and in the surface glycoprotein.
Asunto(s)
Antígenos Virales/análisis , Virus Oncogénicos/inmunología , Línea CelularRESUMEN
Human adenovirus type 16 was studied in Syrian hamsters bearing SV40 virus induced transplantable tumour and in mice of Black line with Lewis cancer. Despite its tropism to the tumour tissue, the virus injected into the blood stream was first accumulated in the lung tissue and in organs of the reticuloendothelial system (the spleen and liver). When the tumour was in the lung tissue of mice, the virus content in it was much higher than in a similar tumour of subcutaneous localization.