RESUMEN
BACKGROUND: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. MATERIALS AND METHODS: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null- transfected HepG2 cell lines. RESULTS: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. CONCLUSIONS: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.
Asunto(s)
Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/patología , Proliferación Celular/efectos de los fármacos , Resistencia a Antineoplásicos/genética , Neoplasias Hepáticas/patología , Proteínas Ribosómicas/genética , Carcinoma Hepatocelular/tratamiento farmacológico , Carcinoma Hepatocelular/genética , Guanidinas/farmacología , Células Hep G2 , Humanos , Neoplasias Hepáticas/tratamiento farmacológico , Neoplasias Hepáticas/genética , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Ribosómicas/metabolismoRESUMEN
OBJECTIVE: To evaluate calcium deficiency and demand in pre-puberty twins and to analyze the heritability of serum bone alkaline phosphatase (BALP). METHODS: A total of 73 pairs of twins aged 9-16 years were examined by BALP test. Microsatellite polymorphism was used to diagnose the zygosity of twins, while both intraclass correlation coefficient method and Christian formula were performed to investigate heritability of serum BALP. RESULTS: The results of zygosity diagnosis displayed that 34 pairs of twins were monozygotic (MZ) twins and 39 pairs were dizygotic (DZ) twins. 97.9% of the subjects appeared unusual in the activity of BALP, with activity of BALP > 250 U/L in 43.1% of subjects and 200-250 U/L in 54.8% of the subjects. The intake of calcium was unsatisfied in 48.4% of the boys and 39.0% in girls. Less than 10.0% of the subjects were satisfied with the intake of calcium in each age group while over 45.0% of the subjects in 10-13 age group were deficient in calcium. Differences between the means of BALP in different sex groups (t = 1.633, P = 0.105) and different age groups (F = 0.323, P = 0.924) were not statistically significant. Heritability analysis displayed that intra-class variation, inter-class variation, intra-class correlation coefficient were 191.54, 1462.22, 0.77 in MZ twins, and those were 491.03, 1475.57, 0.50 in DZ twins respectively with the heritability of BALP activity as 0.54. CONCLUSION: Calcium deficiency is commonplace in pre-puberty twins. Our data showed that the BALP activity was influenced both by genetic (54%) and environmental (46%) factors.